A5090643088- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Advanced MRI Techniques and Applications
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Autism Spectrum Disorder Research
- Neuroscience and Neuropharmacology Research
- Advanced NMR Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- NMR spectroscopy and applications
- Reproductive Physiology in Livestock
- Advanced biosensing and bioanalysis techniques
- Ion channel regulation and function
- Child Nutrition and Feeding Issues
- Viral Infections and Immunology Research
- Cardiovascular and exercise physiology
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer Genomics and Diagnostics
- Body Composition Measurement Techniques
- Hemoglobin structure and function
- Atomic and Subatomic Physics Research
- Sperm and Testicular Function
- Genetic and phenotypic traits in livestock
- Nutrition and Health in Aging
University of Yamanashi
2024-2025
Takeda (Japan)
2025
Hirosaki University
2010-2024
Hamamatsu University
2020
Tokai University
1990-2020
Hamamatsu University School of Medicine
2015-2020
Nihon University
2010-2013
Iwate University
2007
University of Alabama at Birmingham
1991
University of Bremen
1991
We investigated the relationship between nutritional status and muscle energy metabolism during exercise in 18 male patients with chronic obstructive pulmonary disease (COPD) 15 control subjects using 31P nuclear magnetic resonance spectroscopy (31P-MRS). The were further categorized as either a well-nourished (% ideal body weight, % IBW > or = 90) malnourished < state. Muscle was evaluated by determining ratios PCr/(PCr + Pi) (PCr, phosphocreatine; Pi, inorganic phosphate), ATP/(PCr Pi...
Although trans-translation mediated by tmRNA-SmpB has long been known as the sole system to relieve bacterial stalled ribosomes, ArfA recently identified an alternative factor for ribosome rescue in Escherichia coli. This process requires hydrolysis of nascent peptidyl-tRNA RF2, which usually acts a stop codon-specific peptide release factor. It poses fascinating question how and RF2 recognize ribosome. Here, we mapped location directed hydroxyl radical probing. revealed ArfA-binding site...
To add a tag-peptide for degradation to the nascent polypeptide in stalled ribosome, an unusual translation called trans-translation is facilitated by transfer-messenger RNA (tmRNA) having upper half of tRNA structure and sequence encoding except first alanine. During this event, tmRNA enters vacant A-site ribosome without codon-anticodon interaction, but with protein factor SmpB. Here, we studied sites modes binding SmpB directed hydroxyl radical probing from Fe(II) tethered variants. It...
In trans -translation, transfer-messenger RNA (tmRNA), possessing a dual function as tRNA and an mRNA, relieves stalled translation on the ribosome with help of SmpB. Here, we established in vitro system using Escherichia coli -translation factors to evaluate two steps peptidyl transfer from peptidyl-tRNA alanyl-tmRNA resume codon tmRNA. Using this system, effects several mutations upstream tag-encoding region tmRNA were examined. These affected rather than transfer, one them, A84U/U85G,...
During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states low free energy that slowly convert into mature subunits, namely, kinetically trapped particles. Among the factors, circularly permuted GTPase, RsgA, plays a crucial role in maturation decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM employed elucidate RsgA΄s mechanism action. Our results show RsgA destabilizes structure,...
This article describes an apparatus developed by the authors as a substitution for physical therapists regarding mechanical movements in rehabilitation of frozen shoulder. In particular, performance this is improved comparison with existing methods terms following two major points: (1) realization individual patient’s scapula innovative parallel–serial hybrid linkage design and supporting parts that can fix move scapula; (2) addition “teaching” “playback” mode to enable record motion...
Trans -translation relieves a stalled translation on the bacterial ribosome by transfer-messenger RNA (tmRNA) with help of SmpB, an essential cofactor tmRNA. Here, we examined role unstructured C-terminal tail SmpB using in vitro trans system. It was found that truncation or substitution tryptophan residue at 147 middle affected activity early stage -translation. Our investigations also revealed is not required for events until GTP hydrolyzed EF-Tu complex tmRNA-SmpB. A synthetic peptide...
Messenger RNAs lacking a stop codon trap ribosomes at their 3' ends, depleting the pool of available for protein synthesis. In bacteria, remarkable quality control system rescues and recycles stalled in process known as trans-translation. Acting tRNA, transfer-messenger RNA (tmRNA) is aminoacylated, delivered by EF-Tu to ribosomal A site, accepts nascent polypeptide. Translation then resumes on reading frame within tmRNA, encoding short peptide tag that targets degradation proteases. One...
We sought to determine whether nuclear magnetic resonance relaxation times of water in tissue would be useful detect molecular damage lung within 2 weeks after irradiation. Tissue samples were obtained from the lungs rats at various between 1 and 14 days exposure a hemithorax 20 Gy 60Co gamma The spin-lattice time, T1, was measured by inversion recovery method, spin-spin T2, both Hahn spin-echo (Hahn T2) Carr-Purcell-Meiboom-Gill (CPMG methods. T2 could divided into two components, fast...
This case-control study aimed to assess oxidative stress alterations in autism spectrum disorder (ASD). We used the MULTIS method, an electron spin resonance-based technique measuring multiple free radical scavenging activities simultaneously, combination with conventional markers investigate ability of this approach as a non-behavioural diagnostic tool for children ASD. Serum samples 39 ASD and 58 age-matched typical development were analysed. The group showed decreased hydroxyl (·OH)...
Summary Simultaneous measurements of muscle energy metabolism using 31P-magnetic resonance spectroscopy (31P-MRS) and the kinetics muscular oxygen near-infrared (NIRS) were conducted in polymyositis (PM) patients. The subjects 12 PM patients (age 45 ± years) normal controls 41 years). phosphocreatine (PCr) index intracellular pH (pHi) determined with 31P-MRS changes intramuscular oxygenated (oxy-Hb), deoxygenated (deoxy-Hb), total haemoglobin (total Hb) evaluated NIRS . pHi PCr before...
Abstract The effect of liposome‐encapsulated hemoglobin (LEH) was tested in a rodent model limb ischemia and reperfusion—causing local reperfusion injury cascade systemic responses. Intracellular pH (pHi) phosphocreatine (PCr)/inorganic phosphate (Pi) ratio were serially monitored using 31 P‐nuclear magnetic resonance spectroscopy with 2‐cm solenoid coil on hind limb. After baseline measurements, the right underwent for 70 min, followed 10 min later by intravenous administration LEH (10...
Translation termination in bacteria requires that the stop codon be recognized by release factor RF1 or RF2, leading to hydrolysis of ester bond between peptide and tRNA on ribosome. As a consequence, normal cannot proceed if translated mRNA lacks codon. In Escherichia coli, ribosome rescue ArfA releases nascent polypeptide from stalled with help RF2 codon–independent manner. Interestingly, reaction does not is instead provided, even though structures are very similar. Here, we identified...
Since accurate translation from mRNA to protein is critical survival, cells have developed translational quality control systems. Bacterial ribosomes stalled on truncated are rescued by a system involving tmRNA and SmpB referred as trans -translation. Here, we review current understanding of the mechanism Based results obtained using directed hydroxyl radical probing, propose new type molecular mimicry during Besides such chemical approaches, biochemical cryo-EM studies revealed structural...
The serum and fecal testosterone (T) concentrations testicular sizes of two male bharals (Pseudois nayaur) were determined for approximately one year. profiles the T showed a similar tendency as concentrations, there was significant correlation between (r=0.72). rose drastically in October decreased gradually until January. maximum size observed November Semen collected December January excellent quality comparable to domestic sheep goats. active periods testes synchronized with early...
The purpose of present study was to determine annual changes in serum progesterone (P4) concentrations and clarify basic reproductive characteristics, such as breeding season, estrous cycle, puberty female bharals (Pseudois nayaur). Blood collected from 9 once or twice weekly for approximately one year. Serum P4 were determined by radioimmunoassay. showed remarkable cyclic between November/December (winter) May/June (late spring). mean cycle 24.9 ± 0.5 days. Chasing insistently other females...