A5004469840- Ubiquitin and proteasome pathways
- Nuclear Receptors and Signaling
- Iron Metabolism and Disorders
- Apelin-related biomedical research
- Trace Elements in Health
- Endoplasmic Reticulum Stress and Disease
- Molecular Biology Techniques and Applications
- Peptidase Inhibition and Analysis
- CAR-T cell therapy research
- Hippo pathway signaling and YAP/TAZ
- Phagocytosis and Immune Regulation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Toxin Mechanisms and Immunotoxins
- Pancreatic and Hepatic Oncology Research
- Ion Channels and Receptors
- Protein Degradation and Inhibitors
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Cell Image Analysis Techniques
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- Drug Transport and Resistance Mechanisms
- Signaling Pathways in Disease
- Phytochemicals and Antioxidant Activities
- Protease and Inhibitor Mechanisms
- Immune Cell Function and Interaction
Université de Bordeaux
2019-2025
Umeå University
2024
Inserm
2019-2024
Bordeaux Population Health
2020-2024
Apelin is a well-established mediator of survival and mitogenic signaling through the apelin receptor (Aplnr) has been implicated in various cancers; however, little known regarding Elabela (ELA/APELA) signaling, also mediated by Aplnr, its role conversion precursor proELA into mature ELA cancer are unknown. Here, we identified function mTORC1 as an essential that repressed kidney tumor cell growth, migration, survival. Moreover, sunitinib showed synergistic effect repressing growth...
Abstract The adipokine apelin has been directly implicated in various physiological processes during embryogenesis and human cancers. Nevertheless, the importance of conversion its precursor proapelin to mature tumorigenesis remains unknown. In this study, we identify Furin as cellular proprotein convertase responsible for cleavage. We explore therapeutic potential targeting cleavage sites metastatic colorectal cancer by introducing apelin-dm, a modified variant resulting from alteration...
Proprotein convertases or PCs are known to regulate the malignant phenotype of colon cancer cells by different mechanisms, but their effects on stem (CSCs) have been less widely investigated. Here, we report that expression is altered in CSCs, and inhibition activity reduced CSCs growth, survival, invasion three-dimensional spheroid cultures. In vivo, repression general PC inhibitors α1-PDX, Spn4A, decanoyl-RVKR-chloromethylketone (CMK) significantly tumor levels cell markers LGR5 NANOG...
The intracellular calcium concentration ([Ca2+]i) modulation plays a key role in the regulation of cellular growth and survival normal cells failure [Ca2+]i homeostasis is involved tumor initiation progression. Here we showed that inhibition Furin by its naturally occurring inhibitor prodomain ppFurin MDA-MB-231 breast cancer resulted enhanced store-operated entry (SOCE) reduced cell malignant phenotype. Expression stable manner melanoma MDA-MB-435 lines inhibits activity as assessed vitro...
In situ hybridization visualizes RNA in cells, but image analysis is complex. We present a protocol based on open-source software for automated high-content multiplex fluorescence transcriptomics analysis. Steps include nuclei segmentation with Fiji macro and quantification of up to 14 mRNA probes per image. describe procedures storing raw data, quality control images, the use Python app summarize all results one spreadsheet detailing number single or co-positive cells.
The intracellular calcium concentration ([Ca2+]i) modulation plays a key role in the regulation of cellular growth and survival normal cells failure [Ca2+]i homeostasis is involved tumor initiation progression. Here we show that inhibition Furin by its naturally occurring inhibitor prodomain ppFurin MDA-MB-231 breast cancer resulted enhanced SOCE through TRPC6 activation associated reduced malignant phenotype. Expression stable manner melanoma MDA-MB-435 cell lines inhibits activity as...
The pancreatic ductal adenocarcinoma (PDAC) has a bad prognosis with delayed diagnosis that significantly impedes the use of therapeutic resection. Similarly, colorectal cancer (CRC) is often diagnosed at an advanced stage when tumor cell dissemination taken place in liver. A main reason for this situation already resistant or newly tumors to all conventional current therapies. Here, we report re-sensitization PDAC and CRC cells chemotherapy, radiotherapy and/or immune checkpoint inhibitors...