A5100707489- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- Pancreatic and Hepatic Oncology Research
- Viral gastroenteritis research and epidemiology
- Plant Molecular Biology Research
- Liver Disease Diagnosis and Treatment
- Salmonella and Campylobacter epidemiology
- Cancer Diagnosis and Treatment
- Escherichia coli research studies
- Lipid metabolism and disorders
- Phytochemicals and Antioxidant Activities
- Food Safety and Hygiene
- Peptidase Inhibition and Analysis
- Lung Cancer Treatments and Mutations
- Bacterial biofilms and quorum sensing
- Gastric Cancer Management and Outcomes
- Plant Stress Responses and Tolerance
- Pharmacogenetics and Drug Metabolism
- Lymphoma Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Circular RNAs in diseases
- Pancreatitis Pathology and Treatment
- Polyamine Metabolism and Applications
- Sulfur Compounds in Biology
- Ocular Surface and Contact Lens
Shenyang Sport University
2013-2024
Southwest Forestry University
2022-2024
State Forestry and Grassland Administration
2024
Ningxia Medical University
2024
Jiangsu Province Hospital
2023-2024
Nantong University
2010-2024
Guangdong Pharmaceutical University
2022-2024
Affiliated Hospital of Nantong University
2010-2024
China Medical University
2024
Nanjing Medical University
2023-2024
Drought and salinity are two major environmental factors limiting crop production worldwide. Improvement of drought salt tolerance crops with transgenic approach is an effective strategy to meet the demand ever-growing world population. Arabidopsis ENHANCED DROUGHT TOLERANCE1/HOMEODOMAIN GLABROUS11 (AtEDT1/HDG11), a homeodomain-START transcription factor, has been demonstrated significantly improve in Arabidopsis, tobacco, tall fescue rice. Here we report that AtHDG11 also confers upland...
The degradation pathway of glutathione (GSH) in plants is not well understood. In mammals, GSH predominantly metabolized through the gamma-glutamyl cycle, where degraded by sequential reaction transpeptidase (GGT), cyclotransferase, and 5-oxoprolinase to yield glutamate (Glu) dipeptides that are subject peptidase action. this study, we examined if same Arabidopsis (Arabidopsis thaliana) as occurs mammals. Arabidopsis, oxoprolinase knockout mutants (oxp1-1 oxp1-2) accumulate more 5-oxoproline...
The ever-increasing incidence of obesity and related disorders impose serious challenges on public health worldwide. Brown adipose tissue (BAT) has strong capacity for promoting energy expenditure shown great potential in treating obesity. Exosomes are nanovesicles that share the characteristics their donor cells. Whether BAT derived exosomes (BAT-Exos) might exert similar metabolic benefits is worthy investigation. Methods: Obese mice were established by high-fat-diet (HFD) feeding treated...
ABSTRACT Biofilms from drains in food processing facilities with a recent history of no detectable Listeria monocytogenes floor were cultured for microorganisms producing antilisterial metabolites. A total 413 microbial isolates obtained 12 drain biofilm samples and assayed at 15 37°C activities that bactericidal or inhibitory to L. , by two agar plate assays. Twenty-one 257 bacterial 3 156 yeast had activity. All 24 which produced metabolites activity coinoculated broth cultures containing...
gamma-Glutamyl transpeptidase (GGT) is the only enzyme known that can cleave gamma-peptide bond between glutamate and cysteine in glutathione, therefore a key step glutathione degradation. There are three functional GGT genes Arabidopsis, two of which considered here. GGT1 GGT2 apoplastic, associated with plasma membrane and/or cell wall. RNA blots analysis activity knockout mutants suggest expressed most strongly leaves but found throughout plant. A GGT1::GUS fusion construct showed...
Summary γ ‐Glutamyl transpeptidase (GGT) is the enzyme responsible for breaking ‐glutamyl bond between Glu and Cys in glutathione (GSH). We are using this gene family to study GSH degradation plants. There four putative GGT genes Arabidopsis, one of them, GGT3 (At4g29210), analyzed study. localized vacuole based on organelle‐targeting programs, subcellular distribution GFP fusion proteins during transient expression onion ( Allium cepa ) epidermal tissues, its ability metabolize vacuolar...
Hypertrophic scars (HS) are a variety of skin tissue fibrosis disease that occurs in human skin, the effective therapeutic method which is still inaccessible up to now. As bioactive constituent well-known medical plant, Salvia miltiorrhiza (Danshen Chinese), tanshinone IIA (TSA) reported inhibit cell proliferation HS. Therefore, aim this study was prepare TSA self-soluble microneedles strengthen its dermal retention and break through difficulty significantly thickening epidermal connective...
Abstract Photosynthesis is sensitive to environmental stress and must be efficiently modulated in response abiotic stress. However, the underlying mechanisms are not well understood. Here we report that ARABIDOPSIS THALIANA HOMEOBOX 17 (ATHB17), an Arabidopsis HD-Zip transcription factor, regulated expression of a number photosynthesis associated nuclear genes (PhANGs) involved light reaction ATSIG5 ATHB17 was responsive ABA multiple treatments. -overexpressing plants displayed enhanced...
Pyroptosis provides a new window for relieving the tumor immunosuppressive microenvironment (TIM) and promoting systemic immune responses treatments. However, gasdermin D (GSDMD), key protein in pyroptosis process mediated by caspase-1, is low expressed majority of cells small-molecule inhibitors DNA methylation suffer from nonspecific or single-function defects. To address these issues, hexahistidine (His
ABSTRACT In previous studies workers determined that two lactic acid bacterium isolates, Lactococcus lactis subsp. C-1-92 and Enterococcus durans 152 (competitive-exclusion bacteria [CE]), which were originally obtained from biofilms in floor drains, are bactericidal to Listeria monocytogenes or inhibit the growth of L. both vitro at 4 37°C. We evaluated efficacy these isolates for reducing spp. contamination drains a plant fresh poultry is processed. Baseline assays revealed mean numbers...
An analytical method of high performance capillary electrophoresis (HPCE) was developed to simultaneously separate and identify the component monosaccharides Angelica sinensis polysaccharide fractions (APFs), named APF1, APF2 APF3. The predominant sugars in APFs were identified as arabinose, glucose, rhamnose, galactose galacturonic acid well trace amount mannose glucuronic acid, fractionation altered significantly distribution APFs. APF3 most active fraction effectively inhibit H2O2-caused...
Abstract A series of high‐throughput binary cloning vectors were constructed to facilitate gene function analysis in higher plants. This vector consists plasmids designed for plant expression, promoter analysis, silencing, and green fluorescent protein fusions localization. These provide efficient utilizing sites λ phage integrase/excisionase. In addition, unique restriction are incorporated a multiple site enable replacement. The entire available with complete sequence information detailed...
The objective of the present study was to evaluate CA19-9 and CK8/18 expression patterns in pancreatic cancer cell lines induced by 5-fluorouracil (5-Fu), circulating tumor cells (CTCs) peripheral blood patients previously untreated with advanced cancer. Furthermore, goal test relationship dynamic CTCs effects first cycle chemotherapy. To accomplish this study, CD45 antibody coated beads were used discard white blood. This done combination CA19-9-Alexa488 CK8/18-Alexa594 immunofluorescence...