A5071210426- Cancer Immunotherapy and Biomarkers
- Mesenchymal stem cell research
- Neonatal Respiratory Health Research
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Occupational and environmental lung diseases
- Cancer Cells and Metastasis
- RNA modifications and cancer
- Tissue Engineering and Regenerative Medicine
- Cancer Genomics and Diagnostics
- 3D Printing in Biomedical Research
- Cancer-related Molecular Pathways
- Congenital Diaphragmatic Hernia Studies
- Phagocytosis and Immune Regulation
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Respiratory Support and Mechanisms
- Nitric Oxide and Endothelin Effects
- Immune cells in cancer
- CAR-T cell therapy research
- Heme Oxygenase-1 and Carbon Monoxide
- Renal and related cancers
- Respiratory viral infections research
University of Bern
2015-2025
University Hospital of Bern
2015-2025
Harvard University Press
2002-2025
Harvard University
2009-2025
Gillies McIndoe Research Institute
2020-2021
McGill University
2021
Queen's University
1998-2016
Brigham and Women's Hospital
2009-2016
Erasmus MC
2012-2015
Neostem (United States)
2013
Although progenitor cells have been described in distinct anatomical regions of the lung, description resident stem has remained elusive.Surgical lung-tissue specimens were studied situ to identify and characterize human lung cells. We defined their phenotype functional properties vitro vivo.Human lungs contain undifferentiated nested niches distal airways. These are self-renewing, clonogenic, multipotent vitro. After injection into damaged mouse vivo, form bronchioles, alveoli, pulmonary...
The effects of respiration movements on primary lung cells from patients are investigated an easy to handle lung-on-a-chip.
Acute exposure to high-dose gamma radiation due radiological disasters or cancer radiotherapy can result in radiation-induced lung injury (RILI), characterized by acute pneumonitis and subsequent fibrosis. A microfluidic organ-on-a-chip lined human alveolar epithelium interfaced with pulmonary endothelium (Lung Alveolus Chip) is used model RILI vitro. Both exhibit DNA damage, cellular hypertrophy, upregulation of inflammatory cytokines, loss barrier function within 6 h exposure, although...
<i>Objective: </i>To determine whether nutrient intake and academic psychosocial functioning improve after the start of a universal-free school breakfast program (USBP). <i>Methods:</i> Information was gathered from 97 inner city students prior to USBP again had been in place for 6 months. Students who total energy intakes <50% recommended daily allowance (RDA) and/or 2 or more micronutrients RDA were considered be at nutritional risk. <i>Results:</i>...
Abstract The use of mesenchymal stromal cells (MSCs) for treatment bacterial infections, including systemic processes like sepsis, is an evolving field investigation. This study was designed to investigate the potential MSCs, harvested from compact bone, and their interactions with innate immune system, during polymicrobial sepsis induced by cecal ligation puncture (CLP). We also wanted elucidate role endogenous heme oxygenase (HO)-1 in MSCs a infection. bones HO-1 deficient (−/−) wild-type...
Mono- and co-culture spheroids from patient's cells are formed tested under perfusion conditions for prospective personalized oncology applications.
Rationale: Subsets of patients with early-stage lung adenocarcinoma (LUAD) have a poor post-surgical course after curative surgery. However, biomarkers stratifying this high-risk subset and molecular underpinnings underlying the aggressive phenotype remain unclear. Methods: We integrated bulk single-cell transcriptomics, proteomics, secretome spatial profiling clinical LUAD samples to identify that promote phenotype. Results: identified validated THBS2, at multi-omic levels, as tumor...
Objectives: Mesenchymal stromal cells are being investigated as a cell-based therapy for number of disease processes, with promising results in animal models systemic inflammation and sepsis. Studies ongoing to determine ways further improve the therapeutic potential mesenchymal cells. A gas molecule that improves outcome experimental sepsis is carbon monoxide. We hypothesized preconditioning monoxide ex vivo would promote benefit when administered after onset polymicrobial mice. Design:...
The formation of blood vessels is a complex tissue-specific process that plays pivotal role during developmental processes, in wound healing, cancer progression, fibrosis, and other pathologies. To study vasculogenesis vascular remodeling the context lung, we developed an vitro microvascular model closely mimics human lung microvasculature terms three-dimensional architecture, accessibility, functionality, cell types. Human pericytes from distal airway were isolated characterized using flow...
(1) Inactivation of the tumor suppressor NF2 is believed to play a major role in pathogenesis malignant pleural mesothelioma (MPM) by deregulating Hippo-YAP signaling pathway. However, has functions beyond regulation Hippo pathway, raising possibility that contributes MPM via Hippo-independent mechanisms. (2) We performed weighted gene co-expression analysis (WGCNA) transcriptomic and proteomic datasets obtained from The Cancer Gene Atlas (TCGA) cohort identify clusters co-expressed genes...
We reported previously that predelivery of heme oxygenase-1 (HO-1) gene to the heart by adeno-associated virus-2 (AAV-2) markedly reduces ischemia and reperfusion (I/R)-induced myocardial injury. However, effect preemptive HO-1 delivery on long-term survival prevention postinfarction failure has not been determined. assessed survival, function, left ventricular (LV) remodeling 1 yr after infarction (MI) using echocardiographic imaging, pressure-volume (PV) analysis, histomorphometric...
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The respiratory tract with its ease of access, vast surface area and dense network antigen-presenting cells (APCs) represents an ideal target for immune-modulation. Bio-mimetic nanocarriers such as virosomes may provide immunomodulatory properties to treat diseases allergic asthma. In our study we employed a triple co-culture model epithelial cells, macrophages dendritic simulate the human airway barrier. cell line 16HBE was grown on inserts supplemented blood monocyte-derived (MDMs) (MDDCs)...
Pericytes represent important support cells surrounding microvessels found in solid organs. Emerging evidence points to their involvement tumor progression and metastasis. Although reported be present the human lung, specific presence functional orientation within microenvironment non-small cell lung cancer (NSCLC) has not yet been adequately studied. Using a multiparameter approach, we prospectively identified, sorted expanded mesenchymal from primary NSCLC samples based on co-expression of...
Standard treatment for advanced malignant pleural mesothelioma (MPM) is a cisplatin/pemetrexed (MTA) regimen; however, this confronted by drug resistance. Proteotoxic stress in the endoplasmic reticulum (ER) hallmark of cancer and some rely on signalling response to cytotoxic chemotherapeutics. We hypothesise that ER adaptive unfolded protein (UPR) play role chemotherapy resistance MPM. In vitro three-dimensional (3D) ex vivo organotypic culture were used enrich chemotherapy-resistant...
Machine perfusion is rapidly becoming the standard for graft preservation and provides new opportunities organ salvage, reconditioning, repair. Based on their immune-modulatory regenerating properties,1 arguable mesenchymal stromal cells (MSCs) are most promising cell therapy repair pump, though proof of concept still lacking.2 Here, we show feasibility delivering clinically relevant numbers human MSCs (hMSCs; 5–10 × 106/kg) during 30-minute hypothermic oxygenated machine in porcine liver...
Malignant pleural mesothelioma (MPM) is a lethal cancer with limited treatment options. No targeted therapy has emerged yet. Here, we performed an integrated molecular characterization of patient tumors in the TCGA dataset, and discovered that endoplasmic reticulum (ER) stress adaptive unfolded protein response (UPR) signaling are characteristically deregulated MPM. Consequently, pharmacological perturbation ER stress/UPR axis by HA15, agent induces persistent proteotoxic ER, selectively...
Cisplatin plus pemetrexed combination therapy is considered the standard treatment for patients with advanced, non-squamous, non-small-cell lung cancer (NSCLC). However, advanced NSCLC has a 5-year survival rate of below 10%, which mainly due to resistance. We previously showed that cell line A549 harbors different subpopulations including mesenchymal-like subpopulation characterized by increased chemo- and radiotherapy Recently, resistance in hematological solid tumors been associated...