A5039127180- Occupational and environmental lung diseases
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Cellular transport and secretion
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Endoplasmic Reticulum Stress and Disease
- Cancer Genomics and Diagnostics
- Fibroblast Growth Factor Research
- Medical Imaging and Pathology Studies
- Lung Cancer Research Studies
- Tissue Engineering and Regenerative Medicine
- Lipid Membrane Structure and Behavior
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- CRISPR and Genetic Engineering
- Autophagy in Disease and Therapy
- Neuroblastoma Research and Treatments
- Virus-based gene therapy research
- Pleural and Pulmonary Diseases
University Hospital of Bern
2016-2025
University of Bern
2016-2025
Shanghai Chest Hospital
2021
Shanghai Jiao Tong University
2021
Centre of Biomedical Research
2021
ETH Zurich
2008-2011
Board of the Swiss Federal Institutes of Technology
2011
Bioengineering (Switzerland)
2008
Max Planck Society
2007
Max Planck Institute for Biophysical Chemistry
1999-2005
An implanted device using blue-light–triggered expression of the glucagon-like peptide 1 attenuates diabetes in mice.
This study reports on a microfluidic platform which single multicellular spheroids from malignant pleural mesothelioma (MPM), an aggressive tumor with poor prognosis, can be loaded, trapped and tested for chemotherapeutic drug response.
Intrinsic or acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) is common, thus strategies for the management of EGFR-TKIs are urgently required. Ferroptosis a recently discovered form cell death that has been implicated in tumorigenesis and treatment. Accumulating evidence suggests ferroptosis can be therapeutically exploited treatment solid tumors; however, whether targeted treat EGFR mutant lung cancer and/or overcome still unknown.The effect...
Rationale: Subsets of patients with early-stage lung adenocarcinoma (LUAD) have a poor post-surgical course after curative surgery. However, biomarkers stratifying this high-risk subset and molecular underpinnings underlying the aggressive phenotype remain unclear. Methods: We integrated bulk single-cell transcriptomics, proteomics, secretome spatial profiling clinical LUAD samples to identify that promote phenotype. Results: identified validated THBS2, at multi-omic levels, as tumor...
Abstract Lactate dehydrogenase B (LDHB) fuels oxidative cancer cell metabolism by converting lactate to pyruvate. This study uncovers LDHB’s role in countering mitochondria-associated ferroptosis independently of lactate’s function as a carbon source. LDHB silencing alters mitochondrial morphology, causes lipid peroxidation, and reduces viability, which is potentiated the inducer RSL3. Unlike LDHA, acts parallel with glutathione peroxidase 4 (GPX4) dihydroorotate (DHODH) suppress decreasing...
Fusion of transport vesicles with their target organelles involves specific membrane proteins, SNAREs, which form tight complexes bridging the membranes to be fused. Evidence from yeast and mammals indicates that Sec1 family proteins act as regulators fusion by binding SNAREs. In experiments purified we now made observation ER Golgi core SNARE complex could assembled on syntaxin Sed5p tightly bound Sec1-related Sly1p. Sly1p also preassembled in vitro was found part a vesicular/target...
Abstract Anticancer therapies currently used in the clinic often can neither eradicate tumor nor prevent disease recurrence due to resistance. In this study, we showed that chemoresistance pemetrexed, a multi-target anti-folate (MTA) chemotherapeutic agent for non-small cell lung cancer (NSCLC), is associated with stem cell-like phenotype characterized by an enriched gene signature, augmented aldehyde dehydrogenase activity and greater clonogenic potential. Mechanistically, MTA requires...
Abstract Once considered a waste product of anaerobic cellular metabolism, lactate has been identified as critical regulator tumorigenesis, maintenance, and progression. The putative primary function dehydrogenase B (LDHB) is to catalyze the conversion pyruvate; however, its role in regulating metabolism during tumorigenesis largely unknown. To determine whether LDHB plays pivotal we performed 2D 3D vitro experiments, utilized conventional xenograft tumor model, developed novel genetically...
Cell lines are essential tools to standardize and compare experimental findings in basic translational cancer research. The current dogma states that stem cells feature an increased tumor initiation capacity also chemoresistant. Here, we identified comprehensively characterized three morphologically distinct cellular subtypes the non-small cell lung line A549 challenge dogma. Subtype-specific morphology is maintained during short-term culturing, resulting formation of holoclonal, meroclonal,...
Pharmacological inhibition of PAI-1 strongly enhances chemo- and immunotherapeutic response against PDAC.
Drug resistance and tumor heterogeneity are formidable challenges in cancer medicine, which is particularly relevant for KRAS-mutant cancers, the epitome of malignant tumors recalcitrant to targeted therapy efforts first-line chemotherapy. In this study, we delineate that lung cells resistant pemetrexed (MTA) anti-MEK drug trametinib acquire an exquisite dependency on endoplasmic reticulum (ER) stress signaling, rendering selectively susceptible blockage HSP90, receptor tyrosine kinase AXL,...
(1) Inactivation of the tumor suppressor NF2 is believed to play a major role in pathogenesis malignant pleural mesothelioma (MPM) by deregulating Hippo-YAP signaling pathway. However, has functions beyond regulation Hippo pathway, raising possibility that contributes MPM via Hippo-independent mechanisms. (2) We performed weighted gene co-expression analysis (WGCNA) transcriptomic and proteomic datasets obtained from The Cancer Gene Atlas (TCGA) cohort identify clusters co-expressed genes...
Abstract A complex vesicle trafficking system manages the precise and regulated distribution of proteins, membranes other molecular cargo between cellular compartments as well secretion (heterologous) proteins in mammalian cells. Sec1/Munc18 (SM) are key components by regulating membrane fusion. However, it is not clear how SM contribute to overall exocytosis. Here, functional analysis protein Sly1 Munc18c suggested a united, positive impact upon SNARE‐based fusion ER‐to‐Golgi‐...
Mammalian cells play a dominant role in the industrial production of biopharmaceutical proteins. However, productivity producer is often hindered by bottleneck saturated secretory pathway, where sophisticated mechanism vesicle trafficking mediated numerous proteins and their complexes, among which are cross-kingdom conserved SNAREs [soluble NSF (N-ethylmaleimide-sensitive factor) receptor]. The assemble into complexes means four interactive α-helices and, thus, trigger fusion transport...
Lung cancer is the leading cause of cancer-related mortality, and new therapeutic options are urgently needed. Non-small cell lung (NSCLC) accounts for approximately 85 % all cancers, with current standard regimen care NSCLC including chemotherapy pemetrexed as a single agent or in combination platinum-based agents, e.g. cisplatin. Pemetrexed folic acid antagonist that inhibits synthesis precursor nucleotides, whereas cisplatin directly induces DNA adducts, repair which dependent on...
(1) Background: Ferroptosis is an apoptosis-independent cell death program implicated in many diseases including cancer. Emerging evidence suggests ferroptosis as a promising avenue for cancer therapy, but the paucity of mechanistic understanding regulation and lack biomarkers sensitivity to inducers have significantly hampered utility ferroptosis-based therapy. (2) Methods: We performed integrated dataset analysis by correlating small-molecule compounds (n = 481) against transcriptomes...