In Brief Objective: The aim of this study was to evaluate the safety and efficacy black cohosh red clover compared with placebo for relief menopausal vasomotor symptoms. Methods: This a randomized, four-arm, double-blind clinical trial standardized cohosh, clover, placebo, 0.625 mg conjugated equine estrogens plus 2.5 medroxyprogesterone acetate (CEE/MPA; n = 89). Primary outcome measures were reduction in symptoms (hot flashes night sweats) by placebo; secondary outcomes included...

Scope Women seeking alternatives to hormone‐replacement therapy for menopausal symptoms often try botanical dietary supplements containing extracts of hops ( Humulus lupulus L.). Hops contain 8‐prenylnaringenin (8‐PN), a potent phytoestrogen, the related flavanones 6‐prenylnaringenin and isoxanthohumol (IX), prenylated chalcone xanthohumol (XN). Methods results After chemically biologically standardizing an extract spent these marker compounds, escalating dose study was carried out in women...

In response to the need for rapid screening of combinatorial libraries identify new lead compounds during drug discovery, we have developed an on-line combination ultrafiltration and electrospray mass spectrometry, called pulsed which facilitates identification solution-phase ligands in library mixtures that bind receptors. After contained a mixture were bound macromolecular receptor, e.g., human serum albumin or calf intestine adenosine deaminase, ligand-receptor complexes purified by then...

Serotonin receptor (5-HT7) binding assay-directed fractionation of a methanol extract the dried roots Angelica sinensis led to isolation and identification 21 compounds including new phenolic ester, angeliferulate (1), three phthalides, 10-angeloylbutylphthalide (2), sinaspirolide (3), ansaspirolide (4), along with 17 known compounds, p-hydroxyphenethyl trans-ferulate (5), Z-ligustilide (6), Z-butylidenephthalide (7), senkyunolide I (8), Z-6-hydroxy-7-methoxydihydroligustilide (9),...

Abstract The female flowers of hops ( Humulus lupulus L.) used to flavor beer contain the prenylated flavonoids xanthohumol (XN) and isoxanthohumol (IX). IX is moderately estrogenic in vitro XN has pharmacological properties that might make it useful as a cancer chemopreventive agent. metabolism these dietary was investigated using human liver microsomes. Hydroxylation prenyl methyl group primary route oxidative forming either cis or trans hydroxylated metabolites but only isomer XN. double...

Because the prevailing form of hormone replacement therapy is associated with development cancer in breast and endometrial tissues, alternatives are needed for management menopausal symptoms. Formulations Trifolium pratense L. (red clover) being used to alleviate menopause-associated hot flashes but have shown mixed results clinical trials. The strobiles Humulus lupulus (hops) been reported contain prenylflavanone, 8-prenylnaringenin (8-PN), as most estrogenic constituent, this was confirmed...

The photocatalytic degradation of the organophosphorus insecticide diazinon in aqueous suspensions has been studied by using titanium dioxide as a photocatalyst. was fast process and included formation several intermediates that were identified GC/ion-trap mass spectrometry with EI or CI positive negative ionization mode HPLC/electrospray-QqTOF spectrometry. Since primarily hydroxy derivatives these suspensions, mechanism probably based on hydroxyl radical attack. initial oxidative pathways...

Estrogenicity-directed fractionation of a methanol extract the strobiles Humulus lupulus that had been extracted previously with supercritical CO(2), known as "spent hops", led to isolation and identification 22 compounds including 12 prenylated chalcones (1-8, 10-13), five prenylflavanones (14-17), 4-hydroxybenzaldehyde (18), sitosterol-3-O-beta-glucopyranoside (19), humulinone (20), cohumulinone (21). In addition, chalcone xanthohumol C (9a) was obtained 6:1 mixture along its 1' ',2'...

The female flowers of hops (<i>Humulus lupulus</i> L.) are used in the brewing beer and under investigation for use dietary supplements management menopausal symptoms women. Hop extracts contain weakly estrogenic compound isoxanthohumol (IX), proestrogenic xanthohumol, potent estrogen 8-prenylnaringenin (8PN). Because IX can be metabolized human liver to form 8PN, specific cytochrome P450 (P450) enzymes responsible this <i>O</i>-demethylation reaction were identified. In addition, that...

Objectives: To document the chemical and biologic profile of a clinical phase II red clover (Trifolium pratense L.) extract by identifying measuring major minor components visible in high-performance liquid chromatography–ultraviolet (HPLC-UV) chromatogram evaluating each compound for estrogenic antioxidant activity. Design: Individual compounds preformulated (i.e., no excipients present) were identified either isolation followed structure elucidation or matching to retention time molecular...

Cimicifuga racemosa (L.) Nutt. (syn. Actaea L., black cohosh) is used to relieve menopausal hot flashes, although clinical studies have provided conflicting data, and the active constituent(s) mechanism(s) of action remain unknown. Because serotonergic receptors transporters are involved with thermoregulation, cohosh its phytoconstituents were evaluated for activity using 5-HT7 receptor binding, cAMP induction, serotonin selective re-uptake inhibitor (SSRI) assays. Crude extracts displayed...

Detection of drug-drug interactions is essential during the early stages drug discovery and development, understanding drug-botanical important for safe use botanical dietary supplements. Among different forms that are known, inhibition cytochrome P450 (P450) enzymes most common cause or interactions. Therefore, a rapid comprehensive mass spectrometry–based in vitro high-throughput cocktail assay was developed uses 10 substrates simultaneously against nine CYP isoforms. Including probe...

The female flowers of hops are used throughout the world as a flavoring agent for beer. Recently, there has been increasing interest in potential estrogenic properties hop extracts. Among possible compounds hops, 8-prenylnaringenin is perhaps most significant due to its high vitro potency exceeding that other known phytoestrogens. Since data regarding pharmacokinetic this compound lacking, we investigated metabolism by human liver microsomes. A total 12 metabolites were identified, and...

Abstract Hop‐derived products may contain xanthohumol (XN), isoxanthohumol (IX), and the potent phytoestrogen 8‐prenylnaringenin (8‐PN). To evaluate potential health effects of these prenylflavonoids on breast tissue, their concentration, nature metabolites, biodistribution were assessed compared with 17β‐estradiol (E 2 ) exposure. In this dietary intervention study, women randomly allocated to hop ( n =11; 2.04 mg XN, 1.20 IX, 0.1 8‐PN per supplement) or control =10). After a run‐in ≥4...

Since selective inhibition of the inducible form cyclooxygenase (COX-2) might retain all benefits classical nonsteroidal antiinflammatory agents while avoiding major side effects associated with constitutive isoform COX-1, COX-2 has become an important target for discovery and development new drugs. To aid in characterization such inhibitors, we have applied a mass spectrometry-based screening technique, pulsed ultrafiltration spectrometry, using as target. In blind study, 18 samples...

Contents of lead and arsenic were determined in 617 tobacco samples 80 cigarettes. The mean content was 0.93 microg/g (range 0.02-8.56 microg/g) 0.15 < 0.02-2.04 microg/g). cigarettes 1.26 0.02-6.72 0.11 0.02-0.71 micro/g). There a large variability among Positive correlation between contents found (r = 0.22; p 0.0001). Based on our data from literature we compare the other studies discuss influence smoking to exposure health. In conclusion, at same time with implementation use prevention...

A fractionation methodology aimed at the metabolomic mining of new phytoconstituents for widely used botanical, wild yam (Dioscorea villosa), makes use 1D qHNMR and 2D NMR profiles along preparative pathway. This quantifiable structural guidance led to isolation 14 diarylheptanoids (1–14), including five compounds (1–5) with a tetrahydropyrano core skeleton. The structures, absolute configurations both previously known diarylheptanoids, were assigned by combination HRESIMS, NMR, 1H iterative...