A5051344956- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Bacterial biofilms and quorum sensing
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Plant Pathogens and Fungal Diseases
- Antibiotic Resistance in Bacteria
- Essential Oils and Antimicrobial Activity
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Probiotics and Fermented Foods
- Plant Virus Research Studies
- Antimicrobial Peptides and Activities
- Plant tissue culture and regeneration
- Studies on Chitinases and Chitosanases
- Plant Parasitism and Resistance
- Fungal Biology and Applications
- Microbial Community Ecology and Physiology
- Legionella and Acanthamoeba research
- Mycorrhizal Fungi and Plant Interactions
- Pesticide Residue Analysis and Safety
- Salmonella and Campylobacter epidemiology
Nanjing Agricultural University
2016-2025
Institute of Plant Protection
2015-2024
Institute of Agricultural Resources and Regional Planning
2024
Chinese Academy of Agricultural Sciences
2024
Shanghai Electric (China)
2023
Plant (United States)
2020-2022
Zhejiang University
2018
Shaoxing People's Hospital
2018
Jiangsu Academy of Agricultural Sciences
2016-2017
University of Nebraska–Lincoln
2016
HSAF was isolated from Lysobacter enzymogenes, a bacterium used in the biological control of fungal diseases plants. Structurally, it is tetramic acid-containing macrolactam fused to tricyclic system. exhibits novel mode action by disrupting sphingolipids important polarized growth filamentous fungi. Here we describe biosynthetic gene cluster, which contains only single-module polyketide synthase/nonribosomal peptide synthetase (PKS/NRPS), although biosynthesis apparently requires two...
Post-harvest rot causes enormous economic loss to the global kiwifruit industry. Currently, there are no effective fungicides combat disease. It is unclear whether silver nanoparticles (AgNPs) in controlling post-harvest and, if so, what underlying antifungal mechanism is. Our results indicated that 75 ppm AgNPs effectively inhibited mycelial growth and spore germination of four pathogens: Alternaria alternata, Pestalotiopsis microspora, Diaporthe actinidiae, Botryosphaeria dothidea....
Bacterial–fungal interaction (BFI) has significant implications for the health of host plants. While diffusible antibiotic metabolite-mediated competition in BFI been extensively characterized, impact intercellular contact remains largely elusive. Here, we demonstrate that is a prevalent mode between beneficial soil bacteria and pathogenic filamentous fungi. By generating antibiotics-deficient mutants two common bacteria, Lysobacter enzymogenes Pseudomonas fluorescens , show...
ABSTRACT Lysobactor enzymogenes strain OH11 is an emerging biological control agent of fungal and bacterial diseases. We recently completed its genome sequence found it contains a large number gene clusters putatively responsible for the biosynthesis nonribosomal peptides polyketides, including previously identified antifungal dihydromaltophilin (HSAF). One two huge open reading frames, together encoding 12 modules peptide synthetases (NRPS). Gene disruption one NRPS led to disappearance...
Lysobacter enzymogenes is a ubiquitous environmental bacterium that emerging as potentially novel biological control agent and new source of bioactive secondary metabolites, such the heat-stable antifungal factor (HSAF) photoprotective polyene pigments. Thus far, regulatory mechanism(s) for biosynthesis these metabolites remains largely unknown in L. enzymogenes. In present study, diffusible signal (DSF) (DF)-mediated cell-cell signaling systems were identified first time from The results...
Heterocyclic aromatic N-oxides often have potent biological activities, but the mechanism for N-oxidation is unclear. Six phenazine antibiotics were isolated from Lysobacter antibioticus OH13. A 10 gene cluster was identified biosynthesis. Mutation of LaPhzNO1 abolished all N-oxides, while non-oxides markedly increased. homologous to Baeyer–Villiger flavoproteins shown catazlye N-oxidation. and LaPhzS together converted 1,6-dicarboxylic acid 1,6-dihydroxyphenazine N5,N10-dioxide. also...
Enzymes controlling intracellular second messengers in bacteria, such as c-di-GMP, often affect some but not other targets. How specificity is achieved understood only partially. Here, we present a novel mechanism that enables specific c-di-GMP-dependent inhibition of the antifungal antibiotic production. Expression biosynthesis operon for Heat-Stable Antifungal Factor, HSAF, Lysobacter enzymogenes occurs when transcription activator Clp binds to two upstream sites. At high c-di-GMP levels,...
Quorum sensing (QS) in Xanthomonas oryzae pv. oryzicola (Xoc), the causal agent of bacterial leaf streak, is mediated by diffusible signal factor (DSF). DSF-mediating QS has been shown to control virulence and a set virulence-related functions; however, expression profiles functions extracellular proteins controlled DSF remain largely unclear. In present study, 33 DSF-regulated proteins, whose include small-protein mediating QS, oxidative adaptation, macromolecule metabolism, cell structure,...
Vegetable oils are not only major components of human diet but also vital for industrial applications. WRINKLED1 (WRI1) is a pivotal transcription factor governing plant oil biosynthesis, the underlying DNA-binding mechanism remains incompletely understood. Here, we resolved structure Arabidopsis WRI1 (AtWRI1) with its cognate double-stranded DNA (dsDNA), revealing two antiparallel β sheets in tandem AP2 domains that intercalate into adjacent grooves dsDNA to determine sequence recognition...
Lysobacter enzymogenes is a ubiquitous plant-associated and environmentally friendly bacterium emerging as novel biological control agent of plant disease. This produces diverse antifungal factors, such lytic enzymes secondary metabolite (heat-stable factor [HSAF]) having activity with structure mode action. The regulatory mechanisms for biosynthesis factors largely unknown in L. enzymogenes. solo LuxR proteins have been shown to be widespread, playing important roles bacteria. Here, we...
Lysobacter are ubiquitous environmental bacteria emerging as novel biocontrol agents and new sources of anti-infectives. So far, very little effort has been invested in the study biology these Gram-negative gliding bacteria. Many species characterized by their yellow-orange appearance. Using transposon mutagenesis, we identified a stand-alone polyketide synthase (PKS) gene cluster required for pigment production L. enzymogenes OH11. The yellow pigments were abolished "white" mutants...
The study aimed to develop simulation models including intracranial aneurysmal and parent vessel geometries, as well vascular branches, through 3D printing technology. focused on the benefits of treatments clinical education. This prospective included 13 consecutive patients who suffered from aneurysms confirmed by digital subtraction angiography (DSA) in Neurosurgery Department Shaoxing People's Hospital. original 3D-DSA image data were extracted picture archiving communication system...
ABSTRACT Lysobacter enzymogenes is a ubiquitous soil gammaproteobacterium that produces broad-spectrum antifungal antibiotic, known as heat-stable factor (HSAF). To increase HSAF production for use against fungal crop diseases, it important to understand how synthesis regulated. gain insights into transcriptional regulation of the gene cluster, we generated library with deletion mutations in genes predicted encode response regulators two-component signaling systems L. strain OH11. By...
Summary Heat‐stable antifungal factor (HSAF) produced by Lysobacter enzymogenes is a potential lead compound for developing new antibiotics. Yet, how L. regulates the HSAF biosynthesis remains largely unknown. Here, we show that 4‐hydroxybenzoic acid (4‐HBA) serves as diffusible regulating biosynthesis. The of 4‐HBA involved an oxygenase, LenB2, and mutation lenB2 almost completely abolished production, leading to significantly impaired production. Introduction heterologous gene coding...
Heat-stable antifungal factor (HSAF), which belongs to the polycyclic tetramate macrolactam family, was isolated from Lysobacter enzymogenes fermentations and exhibited inhibitory activities against a wide range of fungal pathogens. In this study, activity HSAF Fusarium graminearum in vitro vivo investigated. A total 50% mycelial growth F. suppressed with 4.1 μg/ml (EC50 value). treatment resulted abnormal morphology hyphae, such as curling, apical swelling, depolarized growth. Furthermore,...
Lysobacter enzymogenes is a ubiquitous, beneficial, plant-associated bacterium emerging as novel biological control agent. It has the potential to become new source of antimicrobial secondary metabolites such Heat-Stable Antifungal Factor (HSAF), which broad-spectrum antimycotic with mode action. However, very little information about how L. detects and responds fungi or oomycetes been reported. An in vitro confrontation bioassay between pathogenic oomycete Pythium aphanidermatum biocontrol...