A5047867237- Pancreatic function and diabetes
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
- Neuropeptides and Animal Physiology
- Diet, Metabolism, and Disease
- Birth, Development, and Health
- Diet and metabolism studies
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Receptor Mechanisms and Signaling
- Diabetes and associated disorders
- Rabbits: Nutrition, Reproduction, Health
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Growth Hormone and Insulin-like Growth Factors
- Circadian rhythm and melatonin
- Lipoproteins and Cardiovascular Health
- Nutrition, Genetics, and Disease
- Neonatal Respiratory Health Research
- Neuroendocrine regulation and behavior
- Adenosine and Purinergic Signaling
- Electrolyte and hormonal disorders
- Helicobacter pylori-related gastroenterology studies
- Cancer, Lipids, and Metabolism
- Nitric Oxide and Endothelin Effects
- Polymer Nanocomposites and Properties
Universidad Complutense de Madrid
2005-2022
Hospital Clínico San Carlos
2001-2018
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2009-2018
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2013-2018
Consejo Superior de Investigaciones Científicas
1969-2012
Instituto de Biomedicina y Genética Molecular de Valladolid
2011
Instituto de Química Orgánica General
2008
University of Pavia
2004
Veterans Health Administration
2000
Hospital Infantil Universitario Niño Jesús
2000
Abstract In the present work, several experimental approaches were used to determine presence of glucagon‐like peptide‐1 receptor (GLP‐1R) and biological actions its ligand in human brain. situ hybridization histochemistry revealed specific labelling for GLP‐1 mRNA brain areas. addition, GLP‐1R, glucose transporter isoform (GLUT‐2) glucokinase (GK) mRNAs identified same cells, especially areas hypothalamus involved feeding behaviour. GLP‐1R gene expression gave rise a protein 56 kDa as...
Abstract: This study was designed to determine the possible role of brain glucagon‐like peptide‐1 (GLP‐1) receptors in feeding behavior. In situ hybridization showed colocalization mRNAs for GLP‐1 receptors, glucokinase, and GLUT‐2 third ventricle wall adjacent arcuate nucleus, median eminence, supraoptic nucleus. These areas are considered contain glucose‐sensitive neurons mediating Because GLUT‐2, glucokinase proteins involved multistep process glucose sensing pancreatic β cells, specific...
This study was designed to determine the effects of glucagon-like peptides (GLP) on arterial blood pressure and heart rate. Although glucagon caused a minimal effect GLP-1-(1-37) produced moderate increase both systolic diastolic pressure, GLP-1-(7-36) amide induced greatest increases in parameters. Systolic rate values increased when doses were increased. By contrast, GLP-2 did not modify either or values. To whether mediated through catecholamines, rats pretreated with reserpine,...
Gel filtration studies on Bio-Gel P-10 columns of a 50-fold purified porcine duodenal extract revealed main peak glucagon-like immunoreactivity (GLI) in the 2,900 mol wt zone and smaller 3,500 zone, same as pancreatic glucagon marker. Like glucagon, samples material gave essentially identical measurements radioimmunoassays employing glucagon-specific antiserum 30K GLI crossreacting 78J, whereas peptide 60-fold higher readings 78J assay. On disk gel electrophoresis, fraction, like migrated at...
Although plasma glucagon levels in therat fetus are the adult range, hepatic glycogen ispresent far greater abundance thanin adult. To explain this paradox, adenylatecyclase response to was studied partiallypurified membranes of rat livers obtained throughoutperinatal life and at 3 months age. The glucagon(10-9M) only 7%of day 15 fetal 20%on 21st day. Not until after 30th postpartumdid it reach maturity. Yet, stimulation by NaF comparableto throughout life. binding [125I]iodoglucagon (2 ×...
Abstract: Evidence that glucagon‐like peptide‐1 (GLP‐1) (7–36) amide functions as a novel neuropeptide prompted us to study the gene expression of its receptor in rat brain. Northern blot analysis showed transcripts similar size RINm5F cells, hypothalamus, and brainstem. First‐strand cDNA was prepared by using RNA from brainstem, cells subsequently amplified PCR. Southern PCR products major 1.4‐kb band all these preparations. hypothalamus were cloned, nucleotide sequence one strand identical...
This study was designed to determine the contribution of central nervous system (CNS) effects glucagon-like peptide-1-(7—36) amide (tGLP-1) on arterial blood pressure and heart rate in rats. Accordingly, intracerebroventricular administration peptide produced an increase cardiovascular parameters, which blocked by previous exendin-(9—39) through same route, but not when it intravenously injected. Intravenous tGLP-1 a significant rate, or intravenous exendin-(9—39). Bilateral vagotomy...
Forty-two healthy men and women were subjected to four consecutive dietary periods differing in the fat content of saturated fatty acids (SFAs), monounsaturated (MUFAs), polyunsaturated (n-6) [PUFA(n-6)] (n-3) [PUFA(n-3)]. Plasma lipids, vitamin E, vitro LDL oxidation examined during each period. Adhesion human monocytes cultured endothelial cells was used as a functional test identify differences biological properties from Consumption an SFA-rich diet resulted higher cholesterol (4.06±0.85...
Glucagon-like peptide 1 (GLP-1) functions as an incretin hormone with antidiabetogenic properties. However, the role of GLP-1 in human bone marrow-derived mesenchymal stem cells (hMSCs), if any, remains unknown. The effects on hMSCs were tested regard to cell proliferation, cytoprotection, and differentiation into adipocytes. signaling pathways involved these processes also analyzed. Cells characterized biochemical morphological approaches before after being induced differentiate PCNA...
SUMMARY During the third part of pregnancy in rat concentrations plasma insulin and tissue glycogen foetus increase progressively. These levels release by pancreas incubated vitro were significantly higher than values found adult non-pregnant rats. After birth correlation between concentration stores was also evident. In first day life, insulin, liver, striated muscle kidney decreased significantly; these decrease even more during 15 days lactation. weaning started (20 post partum ), rapid...
SUMMARY The influence of the diet on levels insulin was studied in rats a high-fat diet. Plasma and glucose concentrations control group were compared, so concentration pancreas two groups. mean plasma 40 μ-u./ml. that extracted from 2·5 μg./100 mg. tissue; 156 mg./100 ml. animals fed showed diabetic features. level 9 μ-u./ml., increased to 210 not significantly different controls. In vitro epididymal fat diaphragm high-fat-diet less sensitive than same tissues group.
In an attempt to know the role of pineal gland on glucose homeostasis, blood plasma concentrations glucose, insulin and glucagon under basal conditions or after administration nutrients were studied in jugular vein conscious pinealectomized (Pn), melatonin-treated (Pn + Mel) control (C) rats. Glucose levels smaller C than Pn rats, while immunoreactive (IRI) significantly greater Contrary this, (IRG) animals. Melatonin treatment rats induces increase IRI a reduction IRG levels. Similar...
Abstract: Recently, the description of glucokinase mRNA in certain neuroendocrine cells has opened new ways to characterize this enzyme rat brain. In study, we found and a similar RNA splicing pattern gene product hypothalamus pancreatic islets; that codes for B1 isoform was most abundant, with minor amounts those coding B2, P1, P2, P1/B2, P2/B2 isoforms. Glucokinase expression brain gave rise protein 52 kDa high apparent K m glucose no inhibition by 6‐phosphate, contribution total...
To determine if gastric A-cells are a major source of the glucagonemia insulindeprived depancreatized dogs and to examine their secretory behavior, immunoreactive glucagon (IRG) was measured simultaneously in plasma from inferior vena cava (VC) vein (GV) draining fundus. Basal GV IRG averaged 205 ± 35 pg/ml, significantly above VC level 71 30 (P < 0.001) rose 1417 498 1.5 minutes after start an arginine infusion, exceeding at all points 0.01). Measurement gastric, jejunal, ileal veins...
The truncated form of glucagon‐like peptide‐1 (TGLP‐1, or proglucagon 78–108), secreted by the mammalian intestine, has potent pharmacological activities, stimulating insulin release and inhibiting gastric acid secretion. We have characterized high‐affinity receptors for this peptide in rat isolated fundic glands. Scatchard analysis binding studies using mono‐ 125 I‐TGLP‐l(7–36) amide as tracer showed a single class site K d (4.4±(SE) 0.8) × 10 −10 M, with tissue concentration 1.0 ± 0.1 fmol...
Abstract The glucose transporter isoform‐2 (GLUT‐2) and glucokinase are considered to be components of a sensor system controlling several key processes, hence may modulate feeding behaviour. We have found GLUT‐2 mRNAs in brain regions, including the ventromedial arcuate nuclei hypothalamus. GLUT‐2, regulatory protein proteins were present these areas as determined by biochemical approaches. In addition, glucose‐phosphorylating activity with high apparent K m for that displayed no product...