A5034995655- Computational Drug Discovery Methods
- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- PI3K/AKT/mTOR signaling in cancer
- MicroRNA in disease regulation
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Hepatitis C virus research
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Kruppel-like factors research
- RNA Research and Splicing
- Plant Virus Research Studies
- Protein Tyrosine Phosphatases
- Immunotherapy and Immune Responses
- Genetic Associations and Epidemiology
- Genetic factors in colorectal cancer
- Insect Resistance and Genetics
- Genomics and Rare Diseases
- Essential Oils and Antimicrobial Activity
- Cancer, Lipids, and Metabolism
- Biochemical and Structural Characterization
- Malaria Research and Control
- Plant-Microbe Interactions and Immunity
- Circular RNAs in diseases
University of the Punjab
2022-2025
University of Gujrat
2014-2024
Ecole des Hautes Etudes Commerciales du Nord
2024
University of Lahore
2023
Lahore General Hospital
2022
Quaid-i-Azam University
2015
Achromatopsia (ACHM) is an early-onset retinal dystrophy characterized by photophobia, nystagmus, color blindness and severely reduced visual acuity. Currently mutations in five genes CNGA3, CNGB3, GNAT2, PDE6C PDE6H have been implicated ACHM. We performed homozygosity mapping linkage analysis a consanguineous Pakistani ACHM family mapped the locus to 15.12-Mb region on chromosome 1q23.1-q24.3 with maximum LOD score of 3.6. A DNA sample from affected member underwent exome sequencing. Within...
Abstract Background Polycystic Ovary Syndrome (PCOS) affects a significant proportion of human females worldwide and is characterized by hormonal, metabolic, reproductive dysfunctions, including infertility, irregular menstrual cycles, acanthosis nigricans, hirsutism. Mutations in the estrogen receptor genes ESR1 ESR2 , involved normal follicular development ovulation, can contribute to PCOS. The present study focuses on investigating potential correlation between single nucleotide...
A limited number of studies have tested the effect diets supplemented with black cumin seed (BCS) on performance laying hen.To determine effects various levels dietary BCS hen. BIRDS AND METHODS: Three hundred 40-week-old rural Leghorn (White males X F1 females Fayoumi male Rhode Island Red female) crossbred hens were randomly assigned to four groups five replicates 15 birds each (75 per group), and fed 0%, 3%, 4% or 5% for 60 days. Statistical analysis was performed by means ANOVA.BCS at...
Novel protein kinase C (nPKC) family member, epsilon (PKCε) is an AGC superfamily member. It associated with neurological and metabolic diseases as well human cancers. No study so far has been conducted to identify genetic variations their effect on PKCε folding functioning. The present aimed mutational hotspots in disease-causing non-synonymous variants (nsSNPs) along the investigation of nsSNP impact dynamics. Twenty-nine silico tools were applied determine deleteriousness, dynamics...
ABSTRACT Tomato leaf curl New Delhi virus (ToLCNDV), a begomovirus, that causes severe curling, stunting, and reduced yield in tomato plants is consistently threatening its production worldwide. CRISPR/Cas9‐mediated genome editing has shown immense potential developing disease‐resistant crops. This study successfully focuses on designing precise efficient strategy for planta defence against ToLCNDV. Five key targets within the viral genome, essential replication pathogenicity, were selected....
Breast cancer is one of the most common causes fatalities in females globally. Rising cases drug resistance against existing chemotherapeutics are great problem. To address this issue, there a need to find appropriate biomarker that could be used detect at early stages, so development can avoided. Protein Kinase C iota (PKCɩ), an AGC kinase, has oncogenic role cancers and its expression Single nucleotide polymorphisms (SNPs) have been reported associated with development. So, study aims were...
The breast cancer recurrence and chemoresistance has increased over the years. A novel PKC, PKCε, may promote by causing hypoxia metabolic rewiring. natural flavonoid, Zapotin, in colon cells modulate PKCε expression. Therefore, this study aimed to explore Zapotin impact on expression profile of cells. Pharmacophore analysis was performed molecular dynamics (MD) simulations were employed interaction stability. effect treatment various aspects cell viability metabolism studied MCF-7...
Abstract Background PRKCG encodes PKC γ, which is categorized under the classical protein kinase C family. No studies have specifically established relationship between nsSNPs with structural and functional variations in γ context of hepatocellular carcinoma (HCC). The present study aims to uncover this link through in-silico experimental studies. Methods 3D structure was predicted. Molecular Dynamic (MD) Simulations were run estimates made for interactions, stability, conservation...
A new series of N′-(substituted phenyl)-2-(1-(4-(methylsulfinyl) benzylidene)−5-fluoro-2-methyl-1H-inden-3-yl) acetohydrazide derivatives (1 – 25) were prepared in good yields an efficient manner. All the compounds fully characterised by elemental analysis and spectral data. Synthesised evaluated for antioxidant activity DPPH method. Compounds 7 (R = 3-methoxyphenyl), 3 4-dimethylaminophenyl) 23 2,4,5-trimethoxy phenyl) substitutions found to be having highly potent activity. Compound 3,...
Hepatocellular carcinoma (HCC) is one of the most prevalent types cancer and responsible for close to million annual deaths globally. In Pakistan, HCC accounts 10.7% incidence. Prior studies indicated an association between interleukin 4 (IL-4) cytotoxic T lymphocyte protein (CTLA-4) gene polymorphisms in many cancers, including that are either hepatitis B virus (HBV)- or C Virus (HCV)-induced. The IL-4 CTLA-4 genetic with HCV-induced not yet determined Pakistani population. Therefore, this...
Nematicidal potential of the common plant pathogen Pseudomonas syringae has been recently identified against Caenorhabditis elegans. The current study was designed to investigate detailed genetic mechanism bacterial pathogenicity by applying comparative genomics, transcriptomics, mutant library screening, and protein expression. Results showed that P. strain MB03 could kill C. elegans in liquid assay gut colonization. genome sequenced analysis including multi locus sequence typing,...
Protein kinase C iota (PKC
Untranslated regions of the gene play a crucial role in expression regulation at mRNA and protein levels. Mutations UTRs impact by altering transcription factor binding, transcriptional/translational efficacy, miRNA-mediated regulation, secondary structure, ribosomal translocation, stability. PKCε, serine/threonine kinase, is aberrantly expressed numerous diseases such as cardiovascular disorders, neurological cancers; its probable cause unknown. Therefore, current study, influence PRKCE...
Breast cancer is a leading cause of mortality in Pakistan due to females. has subtypes that inflict differently and owe various factors include our lifestyle genetics. enhanced percentage developing breast fourth decade life women. Numerous genomics proteomics studies are being conducted for prognosis, diagnosis treatment cancer. This review focuses on the mutations BRCA1/2 genes other such genomic biomarkers. Moreover, this emphasizes advancements made proteomic metabolomics carcinoma.
Abstract Hepatocellular carcinoma is a leading cause of cancer-related deaths due to its complexity in diagnosis, chemo-resistance, and aggressive nature. Identifying pathogenic single nucleotide polymorphism (SNP) protein kinase C iota (PKCι) can be potential biomarker the prognosis treatment HCC. This study investigated association between SNP PRKCI Pakistani population's hepatocellular (HCC) risk. Obtained samples were first evaluated for ALT measurements viral load quantification through...
Single nucleotide polymorphisms (SNPs) are associated with many diseases including neurological disorders, heart diseases, diabetes, and different types of cancers. In the context cancer, variations within non-coding regions, UTRs, have gained utmost importance. gene expression, translational regulation is as important transcriptional for normal functioning cells; modification in functions can be pathophysiology diseases. UTR-localized SNPs PRKCI were evaluated using PolymiRTS, miRNASNP,...
Protein Kinase C-epsilon (PKCε) is a member of the novel subfamily PKCs (nPKCs) that plays role in cancer development. Studies have revealed its elevated expression levels are associated with cervical cancer. Previously, we identified pathogenic variations different domains through various bioinformatics tools and molecular dynamic simulation. In present study, aim was to find association variants rs1553369874 rs1345511001 determine influence these on protein-protein interactions PKCε, which...