A5084281774- Climate variability and models
- Oceanographic and Atmospheric Processes
- Single-cell and spatial transcriptomics
- Environmental and Agricultural Sciences
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Gene expression and cancer classification
- Remote Sensing and Land Use
- Cell Image Analysis Techniques
- Gene Regulatory Network Analysis
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Meteorological Phenomena and Simulations
- Cancer-related gene regulation
- Marine and coastal ecosystems
- Environmental Changes in China
- Ocean Waves and Remote Sensing
- Advanced Computational Techniques and Applications
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Embedded Systems and FPGA Design
- Acute Myeloid Leukemia Research
- Air Quality and Health Impacts
- Gambling Behavior and Treatments
- Media Influence and Health
Nanjing University
2024-2025
St. Jude Children's Research Hospital
2023-2025
Zhejiang Chinese Medical University
2024
Hangzhou Hospital of Traditional Chinese Medicine
2024
University of Pittsburgh
2022-2024
Nanfang College Guangzhou
2021-2022
Nanfang Hospital
2021-2022
Shanghai First Maternity and Infant Hospital
2022
Fujian Medical University
2022
Union Hospital
2022
Precise neoepitope discovery is crucial for effective cancer therapeutic vaccines. Conventional approaches struggle to build a repertoire with sufficient immunogenic epitopes. We developed workflow leveraging full-length ribosome–nascent chain complex–bound mRNA sequencing (FL-RNC seq) and artificial intelligence–based predictive models accurately identify the landscape, especially large-scale transcript variants (LSTVs) missed by short-read sequencing. In MC38 mouse model, we identified 22...
Tumour cells often evade immune pressure exerted by CD8+ T or immunotherapies through mechanisms that are largely unclear1,2. Here, using complementary in vivo and vitro CRISPR–Cas9 genetic screens to target metabolic factors, we established voltage-dependent anion channel 2 (VDAC2) as an signal-dependent checkpoint curtails interferon-γ (IFNγ)-mediated tumour destruction inflammatory reprogramming of the microenvironment. Targeting VDAC2 enabled IFNγ-induced cell death cGAS–STING...
Regulatory T cell (Treg) heterogeneity exists in lymphoid and non-lymphoid tissues, but we have limited understanding of context-dependent functions spatiotemporal regulators heterogenous Treg states, especially during perinatal life when immune tolerance is established. Here, revealed that the class III PI3K Vps34 orchestrates effector (eTreg) transitional life. We found loss reduced terminal eTreg accumulation associated with decreased generation tissues development an early-onset...
Cervical cancer (CC) is one of the most common malignant tumors female reproductive system. And immune system disorder in patients results an increasing incidence rate and mortality rate. Pyroptosis system-related programmed cell death pathway that produces systemic inflammation by releasing pro-inflammatory intracellular components. However, diagnostic significance pyroptosis-related genes (PRGs) CC still unclear. Therefore, we identified 52 PRGs from TCGA database screened three...
Introduction To design effective small molecule inhibitors targeting the immune checkpoint PD-1/PD-L1 and to explore their inhibitory activity. Methods In this paper, a total of 69 with same backbone were searched through opendatabases, docking mechanism PD-L1 protein was investigatedby molecular method, active conformation explored. The biological activity four newly designed also evaluated using ELISA. Results most 58 in dataset formed six hydrogen bonds Phe67, Val55, Ile116 Tyr123, while...
PurposeGuanxinshutong capsule (GXST) is a Chinese patent medicine used for the treatment of cardiac fibrosis (CF). Nevertheless, precise mechanism action GXST remains unclear. In this study, we applied network pharmacology, molecular docking, dynamics simulation, and in vivo experiments to assess effects mechanisms CF.MethodsThe TCMSP database provided targets active substances. The GeneCards, OMIM, TTD databases were searched relevant fibrosis. To filter core targets, PPI was set up....
Patients with acute myeloid leukaemia (AML) have poor prognoses and low overall survival (OS) rates owing to its heterogeneity the complexity of tumour microenvironment (TME). N6-methyladenosine (m6A) modification plays a key role in initiation progression haematopoietic malignancies. However, underlying function m6A regulators AML remains elusive. This study thoroughly analysed features 177 patients based on 22 regulators. Utilizing unsupervised clustering, we determined three distinct...
Abstract Many climatolo gists are concerned with the variability of air‐sea interactions. In this paper, signals interannual and interdecadal interactions in southern subtropical Indian Ocean (SSIO) have been extracted from both monthly sea surface temperatures (SST) winds compared to tropical (TIO). Empirical orthogonal function (EOF) expansion has used analysis observed temperature anomalies (SSTA) over basin‐wide (35°S 15°N, 30°E to120°E)for 1950–1998. The results show that another dipole...
Abstract Droplet-based single-cell sequencing techniques rely on the fundamental assumption that each droplet encapsulates a single cell, enabling individual cell omics profiling. However, inevitable issue of multiplets, where two or more cells are encapsulated within droplet, can lead to spurious type annotations and obscure true biological findings. The multiplets is exacerbated in multiomics settings, integrating cross-modality information for clustering inadvertently promote aggregation...
The recent advance of single cell sequencing (scRNA-seq) technology such as Cellular Indexing Transcriptomes and Epitopes by Sequencing (CITE-seq) allows researchers to quantify surface protein abundance RNA expression simultaneously at resolution. Although CITE-seq other similar technologies have gained enormous popularity, novel methods for analyzing this type multi-omics data are in urgent need. A limited number available tools utilize data-driven approach, which may undermine the...
Abstract In this paper, an indicator that shows the maximum subsurface temperature anomalies (MSTA) is first defined to represent thermocline variations through a case analysis of 1997/98. By using past 46‐year (1955–2000) data, oscillation and wave‐signal propagation in tropical Indian Ocean are revealed. The robust spite coincidence with strong El Niño/Southern Oscillation (ENSO) cycle as well anomalous Walker circulation Pacific monsoon Ocean. Due nature wave signals, may be potential...
Abstract Single-cell sequencing has revolutionized our understanding of cellular heterogeneity by offering detailed profiles individual cells within diverse specimens. However, due to the limitations technology, two or more may be captured in same droplet and share barcode. These incidents, termed doublets multiplets, can lead artifacts single-cell data analysis. While explicit experimental design mitigate these issues with help auxiliary cell markers, computationally annotating a broad...
Background/Objectives: Colorectal cancer (CRC) frequently co-occurs with intestinal system diseases (ISDs), yet their molecular interplay remains poorly understood. We employed a comprehensive bioinformatics approach to elucidate shared genetic signatures and pathways between CRC ISDs. Methods: systematically analyzed 12 microarray RNA-seq datasets encompassing 989 samples across seven ISDs CRC. Differentially expressed genes (DEGs) were identified using Limma DESeq2. Functional enrichment...
As an important algorithm, dilate algorithm can give us more connective view of a remote sensing image which has broken lines or objects. However, with the technological progress satellite sensor, resolution been increasing and its data quantities become very large. This would lead to decrease running speed cannot obtain result in limited memory time. To solve this problem, our research proposed parallelized for Image based on MPI MP. Experiments show that method runs faster than traditional...
Sales prediction plays a critical role in the rapid development of internet. Unlike goal predicting physical items sales, virtual video games needn't consider relationship inventory and demand, it reflects consumer preferences to certain extent. Predicting sales game can pre-adjust strategies plans advance. In process predicting, an excellent feature selection method remove features irrelevant enhance accuracy model. this paper, we proposed new hybrid Pearson correlation coefficient - Random...
This article introduced the EDA technology development course, and has made certain description on its basic characteristic process. Meanwhile this detailed introduction to programmable logical component's present situation unique feature. It emphatically elaborated popular FPGA technology. aims at designing an independent experimental platform based component. The hardware part takes ALTERA Corporation's CYCLONE series chips as core, including LED array, seven digital tube display, cluster...