A5067840753- Exercise and Physiological Responses
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Cardiovascular and exercise physiology
- Mitochondrial Function and Pathology
- Antioxidant Activity and Oxidative Stress
- Coenzyme Q10 studies and effects
- Trace Elements in Health
- Vitamin C and Antioxidants Research
- Nutrition and Health in Aging
- Heat shock proteins research
- Cardiac Ischemia and Reperfusion
- Electron Spin Resonance Studies
- Ion channel regulation and function
- Spaceflight effects on biology
- Genetic Neurodegenerative Diseases
- Redox biology and oxidative stress
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Selenium in Biological Systems
- Muscle activation and electromyography studies
- Skin Protection and Aging
- Genetics, Aging, and Longevity in Model Organisms
- Heavy Metal Exposure and Toxicity
- Diet and metabolism studies
Newcastle upon Tyne Hospitals NHS Foundation Trust
2025
Freeman Hospital
2025
University of Liverpool
2015-2024
Versus Arthritis
2020-2024
Brunel University of London
2022
Chartered Institute of Management Accountants
2018
Medical Research Council
1974-2017
In-Q-Tel
2008
Royal Liverpool and Broadgreen University Hospital NHS Trust
2007
Broadgreen Hospital
2007
We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions nuclear, cytosolic mitochondrial compartments. Fractionation allows the analysis protein interest (or other cellular component) based on its compartmental distribution can also generate molecular information about state cell and/or tissue how may differ between different compartments, tissues or types, in response treatments ageing.The described was...
The sources of cytosolic superoxide in skeletal muscle have not been defined. This study examined the subcellular sites that contribute to mature single fibers at rest and during contractile activity.Isolated from mouse flexor digitorum brevis loaded with nitric-oxide-sensitive fluorescent probes, specific pathway inhibitors immunolocalization techniques were used identify contributing superoxide. Treatment electron transport chain complex III inhibitor, antimycin A, but I rotenone, caused...
Previous studies have reported that oxidizing free radical species are generated during exercise, and there has been considerable interest in the potential effects of these on exercising tissues. We hypothesized contracting skeletal muscle was a major source untrained would adapt to oxidative stress single short period contractile activity by upregulation cytoprotective proteins absence overt cellular damage. Fifteen minutes aerobic found induce rapid release superoxide anions from mouse...
Oxidative stress induces adaptations in the expression of protective enzymes and heat shock proteins (HSPs) a variety tissues. We have examined possibility that supplementation subjects with nutritional antioxidant, vitamin C, influences ability lymphocytes to express HSPs following exposure an exogenous oxidant response skeletal muscle physiological oxidative occurs during exercise vivo. Our hypothesis was elevation tissue C content would reduce oxidant-induced HSP content. Lymphocytes from...
Ageing is associated with skeletal muscle atrophy, a deficit in force generation, an increased susceptibility to contraction-induced injury, and permanent following severe injury.Muscles of young mice adapt rapidly exercise by increase the production heat shock proteins (HSPs), whereas muscles old show severely diminished response.We hypothesized that overexpression HSP70 throughout life would reduce age-related changes function.The maximum tetanic extensor digitorum longus (EDL) adult...
Previous studies in animals have demonstrated that a single period of aerobic exercise induces rise the skeletal muscle activity antioxidant enzymes superoxide dismutase and catalase an increase content heat shock proteins (HSPs). The purpose this study was to examine time course response human activities HSP60 HSP70 after exhaustive, nondamaging exercise. Seven volunteers undertook one-legged cycle ergometry at 70% maximal oxygen uptake for 45 min. Biopsies were obtained from vastus...
Summary Oxidative modification of cellular components may contribute to tissue dysfunction during aging. In skeletal muscle, contractile activity increases the generation reactive oxygen and nitrogen species (ROS). The question whether contraction‐induced ROS is further increased in muscle elderly important since this influences recommendations on their exercise participation. Three different approaches were used examine aging generation. Hind limb muscles adult old mice underwent a 15‐min...
Abstract. The role of calcium‐activated degenerative processes in the efflux enzyme from experimentally damaged mouse muscle has been studied using an isolated soleus preparation. Inhibition mitochondrial activity with dinitrophenol or sodium cyanide was found to cause a large enzyme. This largely prevented by withdrawal extracellular calcium suggesting that overload does not play major damage leading efflux. Treatment muscles variety protease inhibitors had no effect on following exhaustive...
Skeletal muscle aging is characterized by atrophy, a deficit in specific force generation, increased susceptibility to injury, and incomplete recovery after severe injury. The ability of muscles old mice produce heat shock proteins (HSPs) response stress severely diminished. Studies our laboratory using HSP70 overexpressor demonstrated that lifelong overexpression skeletal provided protection against damage facilitated successful mice. mechanisms which provides this are unclear. Aging...
Investment in reproduction is costly and frequently decreases survival or future reproductive success. However, the proximate underlying causes for this are largely unknown. Oxidative stress has been suggested as a cost of several studies have demonstrated changes antioxidants with investment. Here, we test whether oxidative consequence female house mice ( Mus musculus domesticus ), which extremely high energetic demands during reproduction, particularly through lactation. Assessing damage...
Abstract Age-related loss of skeletal muscle mass and function is a major contributor to morbidity has profound effect on the quality life older people. The potential role age-dependent mitochondrial dysfunction cumulative oxidative stress as underlying cause aging remains controversial topic. Here we show that pharmacological attenuation age-related redox changes in with SS31 associated some improvements damage mitophagy muscles old mice. However, this treatment failed rescue fiber atrophy...