A5020273366- Exercise and Physiological Responses
- Adipose Tissue and Metabolism
- Heat shock proteins research
- Muscle metabolism and nutrition
- DNA Repair Mechanisms
- Genetics and Neurodevelopmental Disorders
- Cardiovascular and exercise physiology
- Childhood Cancer Survivors' Quality of Life
- Carcinogens and Genotoxicity Assessment
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Extracellular vesicles in disease
- Thermoregulation and physiological responses
- Effects of Radiation Exposure
- Epigenetics and DNA Methylation
- Systemic Sclerosis and Related Diseases
- Cardiovascular Effects of Exercise
- Vitamin C and Antioxidants Research
- Telomeres, Telomerase, and Senescence
- Virus-based gene therapy research
- Cancer survivorship and care
- RNA modifications and cancer
- Redox biology and oxidative stress
- Growth Hormone and Insulin-like Growth Factors
- Coenzyme Q10 studies and effects
Foro Italico University of Rome
2016-2025
Sapienza University of Rome
1991-2015
Department of Medical Sciences
2011
University of Rome Tor Vergata
1992-2006
Institute of Cell Biology and Neurobiology
1998
McMaster University
1990
We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions nuclear, cytosolic mitochondrial compartments. Fractionation allows the analysis protein interest (or other cellular component) based on its compartmental distribution can also generate molecular information about state cell and/or tissue how may differ between different compartments, tissues or types, in response treatments ageing.The described was...
Oxidative stress represents a significant contributing factor to male infertility and sperm dysfunction. In this condition, an increase in ROS production exceeds the body's antioxidant defences, resulting decline spermatozoa quality fertilising capacity. Furthermore, excessive has been linked promotion of genomic damage, lipid peroxidation, inflammation, altered enzyme activity, ultimately, irreversible alterations, cell death, seminal parameters associated with infertility. It is...
Purpose Polymorphic variation in the angiotensin-converting enzyme (ACE) and α-actinin-3 (ACTN3) genes has been reported to be associated with endurance and/or power-related human performance. Our aim was investigate whether polymorphisms ACE ACTN3 are elite swimmer status Caucasian East Asian populations. Methods I/D R577X genotyping carried out for 200 swimmers from European, Commonwealth, Russian, American cohorts (short middle distance, ≤400 m, n = 130; long >400 70) 326 Japanese...
Although several studies have shown that immune cells stimulated by in vitro stress are capable to produce neurotrophins, there is still no evidence whether physiological stress, such as exercise, can modulate the vivo levels of brain-derived neurotrophic factor (BDNF) peripheral blood mononuclear (PBMCs).This work investigated acute exercise modulates expression BDNF, pro-BDNF, and p75(NTR) PBMCs 10 healthy young men who performed a cycling incremental test exhaustion (MAX) or exercised at...
Platelet-rich plasma (PRP) has received increasing interest in applied medicine, being widely used clinical practice with the aim of stimulating tissue healing. Despite reported success, there is still a lack knowledge when considering biological mechanisms at base activity PRP during process muscle The present study was to verify whether local delivery modulates specific molecular events involved early stages regeneration process. right flexor sublimis anesthetized Wistar rats mechanically...
Human aging is associated with a progressive loss of muscle mass and strength concomitant fat accumulation in form inter-muscular adipose tissue, causing skeletal function decline immobilization. Fat can also occur as intra-muscular triglycerides (IMTG) deposition lipid droplets, which are perilipin proteins, such Perilipin2 (Plin2). It not known whether Plin2 expression changes age if this has consequences on strength. We studied the vastus lateralis (VL) both healthy subjects patients...
Regular physical activity is effective as prevention and treatment for different chronic conditions related to the ageing processes. In fact, a sedentary lifestyle has been linked worsening of cellular biomarkers such telomere length (TL) and/or specific epigenetic changes (e.g. DNA methylation), with increase propensity aging-related diseases premature death. Extending our previous findings, we aimed test hypothesis that 12 weeks low frequency, moderate intensity, explosive-type resistance...
Cardiovascular diseases (CVD) can cause various conditions, including an increase in reactive oxygen species (ROS) levels that decrease nitric oxide (NO) availability and promote vasoconstriction, leading to arterial hypertension. Physical exercise (PE) has been found be protective against CVD by helping maintain redox homeostasis through a ROS levels, achieved increased expression of antioxidant enzymes (AOEs) modulation heat shock proteins (HSPs). Extracellular vesicles (EVs) circulating...
We recently demonstrated that low frequency, moderate intensity, explosive-type resistance training (EMRT) is highly beneficial in elderly subjects towards muscle strength and power, with a systemic adaptive response of anti-oxidant stress-induced markers. In the present study, we aimed to evaluate impact EMRT on oxidative stress biomarkers induced old people (70–75 years) by single bout acute, intense exercise. Sixteen randomly assigned either control, not exercising group (n=8) or trained...
Aging is associated with a gradual loss of muscle mass termed sarcopenia, which has significant impact on quality-of-life. Because oxidative stress proposed to negatively upon musculoskeletal aging, we investigated links between human aging and markers stress, relationships strength in young old nonsarcopenic sarcopenic adults. Sixteen 16 males (further subdivided into "old" "old sarcopenic") were studied. The abundance protein carbonyl adducts within skeletal sarcoplasmic, myofibrillar,...
This study aims to evaluate the effectiveness of two school-based physical education (PE) programmes (exercise-based and games-based) compared with traditional PE, on health- skill-related fitness components in children Tirana, Albania.Participants were 378 first-grade (6.8 years) 389 fourth-grade (9.8 attending four randomly selected schools Tirana. Twenty-four school classes within these (stratified by grade) participate as exercise group (EG), games (GG) control (CG). Both EG GG...
Objective. Hyperglycemia leads to increased production of reactive oxygen species (ROS) in type 2 diabetes, which reduces cellular antioxidant defenses and induces DNA lesions. The aim this study was investigate the effects on redox homeostasis oxidative damage exercise training patients with diabetes compared nondiabetic individuals. Methods Results. 12 sedentary diabetic males (62.1 ± 4.3 yrs) healthy (61.7 3.9 were exposed 4-month moderate training, 3 times per week, evaluate effect...
Most anticancer treatments act on oxidative-stress pathways by producing reactive oxygen species (ROS) to kill cancer cells, commonly resulting in consequential drug-induced systemic cytotoxicity. Physical activity (PA) has arisen as an integrative therapy, having positive health effects, including redox-homeostasis. Here, we investigated the impact of online supervised PA program promoter-specific DNA methylation, and corresponding gene expression/activity, 3 antioxidants- (SOD1, SOD2, CAT)...
Abstract Thioredoxin reductase 1 (TrxR1) is a selenocysteine-containing protein involved in cellular redox homeostasis which downregulated skeletal muscle differentiation. Here we show that TrxR1 decrease occurring during myogenesis functionally the coordination of this process. Indeed, depletion reduces myoblasts growth by inducing an early -related gene expression pattern includes myogenin and Myf5 up-regulation Cyclin D1 decrease. On contrary, overexpression differentiation delays...
Physical activity has been demonstrated to be effective in the prevention and treatment of different chronic conditions, including type 2 diabetes (T2D). In particular, several studies highlighted how beneficial effects physical may related stability DNA molecule, such as longer telomeric ends. Here we analyze effect exercise training on telomere length, spontaneous H2O2-induced damage, well apoptosis level leukocytes from untrained or trained T2D patients vs. age-matched control subjects...
Alternative splicing plays a key role in the DNA damage response and cancer. Ewing Sarcomas (ES) are aggressive tumors caused by different chromosomal translocations that yield in-frame fusion proteins driving transformation. RNA profiling reveals genes differentially regulated UV light irradiation two ES cell lines exhibiting sensitivity to genotoxic stress. In particular, induces new isoform of helicase DHX9 more sensitive SK-N-MC cells, which is targeted nonsense-mediated decay (NMD),...