A5039759348- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
- Diet and metabolism studies
- Cardiovascular Effects of Exercise
- Adipose Tissue and Metabolism
- Gene expression and cancer classification
- Nutrition, Genetics, and Disease
- RNA modifications and cancer
- Lipoproteins and Cardiovascular Health
- Prion Diseases and Protein Misfolding
- Molecular Biology Techniques and Applications
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Cardiac pacing and defibrillation studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Genomic variations and chromosomal abnormalities
- Pharmacogenetics and Drug Metabolism
- Endoplasmic Reticulum Stress and Disease
- Genomics and Rare Diseases
- Alzheimer's disease research and treatments
National and Kapodistrian University of Athens
2016-2025
Biomedical Research Foundation of the Academy of Athens
2016-2025
University General Hospital Attikon
2015-2024
Academy of Athens
2011-2022
Qatar Cardiovascular Research Center
2013-2014
Leiden University Medical Center
2012
Institute of Cardiology
2012
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2012
Medical University of Graz
2012
University College London
2012
Abstract Dermatomyositis has been modeled as an autoimmune disease largely mediated by the adaptive immune system, including a local humorally response with B and T helper cell muscle infiltration, antibody complement‐mediated injury of capillaries, perifascicular atrophy fibers caused ischemia. To further understand pathophysiology dermatomyositis, we used microarrays, computational methods, immunohistochemistry electron microscopy to study specimens from 67 patients, 54 inflammatory...
Batch effects are the systematic non-biological differences between batches (groups) of samples in microarray experiments due to various causes such as sample preparation and hybridization protocols. Previous work focused mainly on development methods for effective batch removal. However, their impact cross-batch prediction performance, which is one most important goals microarray-based applications, has not been addressed. This paper uses a broad selection data sets from Microarray Quality...
Intermittent fasting has been linked to metabolic health by improving lipid profiles, reducing body weight, and increasing insulin sensitivity. However, several randomized clinical trials have shown that intermittent is not more effective than standard daily caloric restriction for short-term weight loss or cardiometabolic improvements in patients with obesity. Observational studies also suggest cardiovascular benefits from extended rather reduced eating windows, indicate long-term regimens...
The primary cause of Duchenne muscular dystrophy (DMD) is a mutation in the dystrophin gene leading to absence corresponding RNA transcript and protein. Absence leads disruption dystrophin-associated protein complex substantial changes skeletal muscle pathology. Although histological pathology dystrophic tissue has been well documented, underlying molecular pathways remain poorly understood. To examine pathogenic identify new or modifying factors involved dystrophy, expression microarrays...
<b><i>Objective: </i></b> To describe the use of large-scale gene expression profiles to distinguish broad categories myopathy and subtypes inflammatory myopathies (IM) provide insight into pathogenesis inclusion body myositis (IBM), polymyositis, dermatomyositis. <b><i>Methods: Using Affymetrix GeneChip microarrays, authors measured simultaneous approximately 10,000 genes in muscle specimens from 45 patients four major disease (dystrophy, congenital myopathy, normal). The separately...
Skeletal muscle differentiation is a complex, highly coordinated process that relies on precise temporal gene expression patterns. To better understand this cascade of transcriptional events, we used profiling to analyze in 12-day time course differentiating C2C12 myoblasts. Cluster analysis specific for time-ordered microarray experiments classified 2895 genes and ESTs with variable levels between proliferating cells into 22 clusters distinct patterns during myogenesis. Expression several...
The origin of the pathogenic endothelial cells in common infantile hemangioma is unknown. We show here that transcriptomes human placenta and are sufficiently similar to suggest a placental for this tumor, expanding on recent immunophenotypical studies have suggested possibility [North, P. E., et al. (2001) Arch. Dermatol. 137, 559-570]. placenta, hemangioma, eight normal diseased tissues were compared by hierarchical nonhierarchical clustering analysis >7,800 genes. found level...
Skeletal muscle contraction is triggered by the excitation-contraction (E-C) coupling machinery residing at triad, a membrane structure formed juxtaposition of T-tubules and sarcoplasmic reticulum (SR) cisternae. The formation maintenance this key for function but not well characterized. We have investigated mechanisms leading to X-linked myotubular myopathy (XLMTM), severe congenital disorder due loss mutations in MTM1 gene, encoding myotubularin, phosphoinositide phosphatase thought role...
A number of genetic mutations is associated with cardiomyopathies. mutation in the coding region phospholamban (PLN) gene (R14del) identified families hereditary heart failure. Heterozygous patients exhibit left ventricular dilation and arrhythmias. Here we generate induced pluripotent stem cells (iPSCs) from a patient harbouring PLN R14del differentiate them into cardiomyocytes (iPSC-CMs). We find that induces Ca(2+) handling abnormalities, electrical instability, abnormal cytoplasmic...
Although its involvement in prion replication and neurotoxicity during transmissible spongiform encephalopathies is undisputed, the physiological role of cellular protein (PrPC) remains enigmatic. A plethora functions have been ascribed to PrPC based on phenotypes Prnp−/− mice. However, all currently available lines were generated embryonic stem cells from 129 strain laboratory mouse mostly crossed non-129 strains. Therefore, Prnp-linked loci polymorphic between backcrossing resulted...
Cardiac contractility is regulated through the activity of various key Ca(2+)-handling proteins. The sarco(endo)plasmic reticulum (SR) Ca(2+) transport ATPase (SERCA2a) and its inhibitor phospholamban (PLN) control uptake by SR membranes during relaxation. Recently, antiapoptotic HS-1-associated protein X-1 (HAX-1) was identified as a binding partner PLN, this interaction postulated to regulate cell apoptosis. In current study, we determined that HAX-1 can also bind SERCA2. Deletion mapping...
This study aimed to identify candidate new diagnosis and prognosis markers medicinal targets of prostate cancer (PCa), using state the art proteomics. A total 20 tissue specimens from 10 patients with benign prostatic hyperplasia (BPH) PCa (Tumour Node Metastasis [TNM] stage T1−T3) were analyzed by isobaric stable isotope labeling (iTRAQ) two-dimensional liquid chromatography−tandem mass spectrometry (2DLC−MS/MS) approaches a hybrid quadrupole time-of-flight system (QqTOF). The resulted in...
Desmin, the muscle-specific intermediate filament protein, is a major target in dilated cardiomyopathy and heart failure humans mice. The hallmarks of desmin-deficient (des(-/-)) mice pathology include pronounced myocardial degeneration, extended fibrosis, osteopontin (OPN) overexpression. We sought to identify molecular cellular events regulating adverse cardiac remodelling des(-/-) their potential link OPN.In situ hybridization, histology, immunostaining demonstrated that inflammatory...
Prions induce lethal neurodegeneration and consist of PrPSc, an aggregated conformer the cellular prion protein PrPC. Antibody-derived ligands to globular domain PrPC (collectively termed GDL) are also neurotoxic. Here we show that GDL infections activate same pathways. Firstly, both infection cerebellar organotypic cultured slices (COCS) induced production reactive oxygen species (ROS). Accordingly, ROS scavenging, which counteracts toxicity in vitro vivo, prolonged lifespan prion-infected...
AimsH2S is known to confer cardioprotection; however, the pathways mediating its effects in vivo remain incompletely understood. The purpose of present study evaluate contribution cGMP-regulated infarct-limiting effect H2S vivo.
The clinical course of prion diseases is accurately predictable despite long latency periods, suggesting that pathogenesis driven by precisely timed molecular events. We constructed a searchable genome-wide atlas mRNA abundance and splicing alterations during the disease in prion-inoculated mice. Prion infection induced PrP-dependent transient changes processing already at eight weeks post inoculation, well ahead any neuropathological signs. In contrast, microglia-enriched genes displayed an...
Abstract Background and aims Treatment with tumor necrosis factor α (TNFα) antagonists in IBD patients suffers from primary non-response rates of up to 40%. Biomarkers for early prediction therapy success are missing. We investigated the dynamics gene expression DNA methylation blood samples treated TNF antagonist infliximab analyzed predictive potential regarding outcome. Methods performed a longitudinal, blood-based multi-omics study two prospective patient cohorts receiving first-time...