A5047933988- Advanced Glycation End Products research
- S100 Proteins and Annexins
- Prion Diseases and Protein Misfolding
- Immune cells in cancer
- Trace Elements in Health
- Chemical Synthesis and Analysis
- Alzheimer's disease research and treatments
- Immune Response and Inflammation
- Ion channel regulation and function
- Neurological diseases and metabolism
- Cancer, Hypoxia, and Metabolism
- Polysaccharides and Plant Cell Walls
- Neonatal Respiratory Health Research
- Receptor Mechanisms and Signaling
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolites in Food Biotechnology
- Food composition and properties
- Machine Learning in Bioinformatics
- Protein Kinase Regulation and GTPase Signaling
- Heme Oxygenase-1 and Carbon Monoxide
- Enzyme Structure and Function
- Nanoplatforms for cancer theranostics
- Protein Structure and Dynamics
- Antimicrobial Peptides and Activities
North Dakota State University
2015-2025
Dakota State University
2011-2020
Wells Fargo (United States)
2016
Florida Atlantic University
2007-2009
Scripps Research Institute
2001-2008
University of Zurich
2006-2007
Vanderbilt University
2007
University of Konstanz
2007
Infectiologie Animale et Santé Publique
2002
ETH Zurich
1998-2001
S100 proteins are EF-hand calcium-binding with various intracellular functions including cell proliferation, differentiation, migration, and apoptosis. Some also secreted exert extracellular paracrine autocrine functions. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating protein-induced cellular signaling. Here we compared interaction of two proteins, S100B S100A6, RAGE by vitro assay culture human SH-SY5Y...
To understand the posttranslational conversion of cellular prion protein (PrPC) to its pathologic conformation, it is important define intracellular trafficking pathway PrPC within endomembrane system. We studied localization and internalization in CHO cells using cryoimmunogold electron microscopy. At steady state, was enriched caveolae both at TGN plasma membrane interconnecting chains endocytic caveolae. Protein A-gold particles bound specifically on live cells. These complexes were...
The receptor for advanced glycation end products (RAGE) is an important cell surface being pursued as a therapeutic target because it has been implicated in complications arising from diabetes and chronic inflammatory conditions. RAGE single membrane spanning containing very small ∼40 residue cytosolic domain large extracellular region composed of 3 Ig-like domains. In this study, high level bacterial expression systems purification protocols were generated the (sRAGE) five permutations...
In the genesis of Alzheimer's disease (AD), converging lines evidence suggest that amyloid-β peptide (Aβ) triggers a pathogenic cascade leading to neuronal loss. It was long assumed Aβ had be assembled into extracellular amyloid fibrils or aggregates exert its cytotoxic effects. Over past decade, characterization soluble oligomeric species in brains AD patients and transgenic models has raised possibility different conformations may contribute pathology via mechanisms. The receptor for...
Abstract CD34 + hematopoietic stem/progenitor cells (HSPCs) are vasculogenic and hypoxia is a strong stimulus for the vasoreparative functions of these cells. Angiotensin‐converting enzyme 2 (ACE2)/angiotensin‐(1–7)/Mas receptor (MasR) pathway stimulates vasoprotective This study tested if ACE2 MasR involved in hypoxic stimulation Cells were isolated from circulating mononuclear derived healthy subjects ( n = 46) exposed to normoxia (20% O ) or (1% ). Luciferase reporter assays carried out...
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of unknown cause that afflicts the central nervous system. MS typified by highly clonally restricted antigen-driven antibody response confined largely to The major antigenic targets this and role in pathogenesis remain unclear. To help resolve these issues, we cloned IgG repertoire directly from active plaque periplaque regions brain B cells recovered cerebrospinal fluid patient with subacute disease. We found...
Increasing evidence suggests that the small EF-hand calcium-binding protein S100B plays an important role in Alzheimer's disease. Among other evidences are increased levels of both and its receptor, Receptor for Advanced Glycation Endproducts (RAGEs) AD diseased brain. The regulation RAGE signaling by is complex probably involves ligands including amyloid beta peptide (A), (AGEs), or transtheyretin. In this paper we discuss current literature regarding S100B/RAGE activation
Advanced glycation endproducts (AGEs) containing carboxymethyllysine (CML) modifications are generally thought to be ligands of the receptor for AGEs, RAGEs. It has been argued that this results in activation pro-inflammatory pathways and diseases. However, it not shown conclusively a CML-modified protein can interact directly with RAGE. Here, we have analyzed whether beta-lactoglobulin (bLG) or human serum albumin (HSA) modified chemically contain only CML (10-40% lysine modification) (i)...
Abstract The ability to map multiple biomarkers at the same time has far‐reaching biomedical and diagnostic applications. Here, a series of biocompatible poly( d,l ‐lactic‐ co ‐glycolic acid) polyethylene glycol particles for multicolor multiplexed imaging are reported. More than 30 particle formulations that exhibit distinct emission signatures (ranging from visible NIR wavelength region) designed synthesized. These encapsulated with combinations carbocyanine‐based fluorophores DiO, Dil,...
Liposomes are representative lipid nanoparticles widely used for delivering anticancer drugs, DNA fragments, or siRNA to cancer cells. Upon targeting, various internal and external triggers have been increase the rate contents release from liposomes. Among triggers, decreased pH within cellular lysosomes has successfully enhance releasing contents. However, imparting sensitivity liposomes requires synthesis of specialized lipids with structures that substantially modified at a reduced pH....
The use of plant derived polysaccharides as health promoters has gained immense interest in the past few years. Arabinoxylan (AX) is predominant non-starch polysaccharide cereals and grasses including wheat. current research aimed to investigate structure-function relationship arabinoxylan hydrolyzates (AXH), obtained by enzymatic hydrolysis AX using xylanase arabinofuranosidase immunomodulators two colon cancer cell lines: Caco-2 HT-29. Fine structural details had a strong correlation with...
Inflammation is an important healthy immune response of the body during lesions and infection. However, uncontrolled excessive inflammation can be damaging to cells. The specific objective this research was evaluate effect structural details enzymatically derived wheat arabinoxylan hydrolyzates (AXH) on their immunomodulatory properties. Out 30 AXH, six AXH showed statistically significant reduction in NO production compared control, causing approximately 24 12% production. Five exhibited...
Abstract Background: The receptor for advanced glycation end products (RAGE) is a pattern recognition that participates to many aspects of tumorigenesis including cancer cell proliferation, migration, invasion. RAGE also contributes resistance chemotherapeutic agents. We have previously shown inhibition, with the small molecule inhibitor (FPS-ZM1), increased efficacy agent dacarbazine, in mouse model melanoma. later showed inhibition enhances cytotoxic gemcitabine an orthotopic pancreatic...