A5037338533- Mosquito-borne diseases and control
- Malaria Research and Control
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- HIV/AIDS Research and Interventions
- Gut microbiota and health
- Molecular Biology Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Biosensors and Analytical Detection
- Single-cell and spatial transcriptomics
- Digital Imaging for Blood Diseases
- T-cell and B-cell Immunology
- Aquaculture disease management and microbiota
- Dengue and Mosquito Control Research
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Long-Term Effects of COVID-19
- HIV/AIDS drug development and treatment
- Vector-borne infectious diseases
- Salivary Gland Disorders and Functions
- Genomics and Rare Diseases
- Antimicrobial Resistance in Staphylococcus
The University of Tokyo
2012-2024
National Institute of Infectious Diseases
2020-2024
Oita University
2014-2020
Here, we report the application of a portable sequencer, MinION, for genotyping malaria parasite Plasmodium falciparum. In present study, an amplicon mixture nine representative genes causing resistance to anti-malaria drugs is diagnosed. First, developed procedure four laboratory strains (3D7, Dd2, 7G8, and K1), then applied ten clinical samples. We sequenced re-sequenced samples using obsolete flow cell R7.3 most recent R9.4. Although average base-call accuracy MinION sequencer was 74.3%,...
A simple and accurate molecular diagnostic method for malaria is urgently needed due to the limitations of conventional microscopic examination. In this study, we demonstrate a new procedure human using loop mediated isothermal amplification (LAMP) MinION™ nanopore sequencer.We generated specific LAMP primers targeting 18S-rRNA gene all five Plasmodium species including two P. ovale subspecies (P. falciparum, vivax, wallikeri, curtisi, knowlesi malariae) examined blood samples collected from...
The recent development of a nanopore-type portable DNA sequencer has changed the way we think about sequencing. We can perform sequencing directly in field, where collect samples. Here, report novel method to detect and genotype tropical disease pathogens, using dengue fever as model. By combining with isothermal amplification that only requires water bath, were able amplify sequence target viral genomes ease. Starting from serum sample, entire procedure could be finished single day....
HIV-1 infected individuals under antiretroviral therapy can control viremia but often develop non-AIDS diseases such as cardiovascular and metabolic disorders. Gut microbiome dysbiosis has been indicated to be associated with progression of these diseases. Analyses gut/fecal in individual regions are important for our understanding pathogenesis infections. However, data on not yet accumulated West Africa. In the present study, we examined fecal compositions adults Ghana, where approximately...
The recent spread of artemisinin (ART)-resistant Plasmodium falciparum represents an emerging global threat to public health. In Southeast Asia, the C580Y mutation kelch13 (k13) is dominant ART-resistant P. falciparum. Therefore, a simple method for detection urgently needed enable widespread routine surveillance in field. aim this study develop new diagnostic procedure using loop-mediated isothermal amplification (LAMP) combined with MinION nanopore sequencer.A LAMP assay k13 gene detect...
Detection and sequencing of chikungunya virus (CHIKV) genome was performed using a combination modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method MinION sequencer. We developed the protocol for drying all reagents RT-LAMP in single reaction tube. Using this system, CHIKV effectively amplified under conditions, used as template with laptop computer. Our in-house system were also validated RNAs serum samples from recent outbreaks patients Brazil. The...
Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates positively or negatively modify amplification in invertebrate host. We used deep sequencing compare differential transcript abundances Aedes aegypti 14 days post infection those uninfected A. aegypti. The gene lethal(2)-essential-for-life [l(2)efl], which encodes member heat shock 20 protein (HSP20) family, was upregulated following type 2 (DENV-2) vivo. transcripts this did...
Recent studies have indicated an association between gut microbiome composition and various disorders, including infectious diseases. The of the differs among ethnicities countries, possibly resulting in diversified interactions host immunity microbiome. Characterization baseline healthy people is essential step for better understanding biological associated with individual populations. However, data on gut/fecal not been accumulated individuals West Africa. In present study, we examined...
Immune responses are different between individuals and personal health histories unique environmental conditions should collectively determine the present state of immune cells. However, molecular systems underlying such heterogeneity remain elusive. Here, we conducted a systematic time-lapse single-cell analysis, using 171 libraries 30 mass cytometry datasets intensively for seven healthy individuals. We found substantial diversity in immune-cell profiles These patterns showed daily...
To evaluate the potential of local mosquitoes to act as vectors for dengue transmission in Japan. Serotype 2 ThNH28/93 was used test susceptibility profiles Aedes flavopictus miyarai (Ae. f. miyarai), galloisi galloisi) and albopictus albopictus), which were collected We aegypti from Thailand a positive control. The infected with virus intrathoracically or orally. At 10 14 days post infection, dissected total RNA extracted their abdomens, thoraxes, heads legs. Mosquito evaluated using RT-PCR...
Human immunodeficiency virus (HIV) 1 infection is known to cause gut microbiota dysbiosis. Among the causes direct of HIV-1 in gut-resident CD4 + T cells, causing a cascade phenomena resulting instability mucosa. The effect HIV on microbiome dysbiosis remains unresolved despite antiretroviral therapy. Here, we show results longitudinal study analysis people living with (PLWH). We contrasted diversity and composition patients at first second time points (baseline_case six months later...
Persistent inflammation during chronic human immunodeficiency virus (HIV) infection may affect the immune response against severe acute respiratory syndrome-coronavirus 2 (SARS- CoV-2) infection. Plasma levels of multiple proinflammatory cytokines SARS-CoV-2 were measured in people with HIV (PWH) effective combination antiretroviral therapy. There no significant differences any between severity coronavirus disease 2019 (COVID-19) PWH, while most significantly higher HIV-uninfected...
Survival strategy of bacteria is expanded by extrachromosomal elements (ECEs). However, their genetic diversity and functional roles for adaptability are largely unknown. Here, we discovered a novel family intracellular ECEs using 56 saliva samples developing an efficient microbial DNA extraction method coupled with long-read metagenomics assembly. Even though this ECE was not hitherto unidentified, our global prevalence analysis 476 salivary metagenomic datasets elucidated that these reside...
Expanding access to effective antiretroviral therapy (ART) is a major tool for management of Human Immunodeficiency Virus (HIV) infection. However, rising levels HIV drug-resistance have significantly hampered the anticipated success ART in persons living with (PLWH), particularly those from Africa. Though great strides been made Ghana toward achieving UNAIDS “95-95-95” target, substantial number PLWH receiving not attained viral suppression. This study investigated patterns drug resistance...
Malaria still poses one of the major threats to human health. Development effective antimalarial drugs has decreased this threat; however, emergence drug-resistant Plasmodium falciparum, a cause Malaria, is disconcerting. The drug chloroquine been effectively used, but resistant parasites have spread worldwide. Interestingly, withdrawal reportedly leads an increased population susceptible in some cases. We examined prevalence genomic polymorphisms malaria parasite P. associated with...
Dengue virus infection manifests in three distinct forms humans: dengue fever, hemorrhagic and shock syndrome. Infection with the is a fatal disease; no vaccine available prevention depends on interruption of chain transmission. The study viral transmission by mosquitoes hindered due to lack an affordable animal model. In general, immuno-competent mice are used as simple inexpensive model, but not susceptible therefore viremia will occur following inoculation such mice. Here, we report...
To disseminate the portable sequencer MinION in developing countries for main purpose of battling infectious diseases, we found a consortium called Global Research Alliance Infectious Diseases (GRAID). By holding and inviting researchers both from developed countries, aim to train participants with MinION's operations foster collaboration diseases researches. As real-life example which resources are limited, describe here result training course, metagenomics analysis two blood samples...
ABSTRACT It is believed that immune responses are different between individuals and at times. In addition, personal health histories unique environmental conditions should collectively determine the present state of cells. However, cellular molecular system mechanisms underlying such heterogeneity remain largely elusive. this study, we conducted a systematic time-lapse single-cell analysis, using 171 libraries 30 mass cytometry datasets intensively for seven healthy individuals. We found...