A5059444942- Monoclonal and Polyclonal Antibodies Research
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- Allergic Rhinitis and Sensitization
- Blood groups and transfusion
- Cell Adhesion Molecules Research
- Chronic Lymphocytic Leukemia Research
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Corneal Surgery and Treatments
- Polysaccharides and Plant Cell Walls
- Skin Protection and Aging
- Cytomegalovirus and herpesvirus research
- Planarian Biology and Electrostimulation
- Circular RNAs in diseases
- Neurobiology and Insect Physiology Research
- HER2/EGFR in Cancer Research
- melanin and skin pigmentation
- Plant Reproductive Biology
- Rheumatoid Arthritis Research and Therapies
- Ocular Surface and Contact Lens
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Hypoxia, and Metabolism
- Neuropeptides and Animal Physiology
University of Pennsylvania
2017-2024
RELX Group (Netherlands)
2020-2022
Max Planck Institute for Molecular Biomedicine
2014-2021
Showa University
2019
Red Cross Hospital
2013
University of Fukui
2013
Japan Science and Technology Agency
2012
RIKEN Advanced Science Institute
2012
Keio University
2012
Nagoya University
2011
Planarians can regenerate their head within days. This process depends on the direction of adult stem cells to wound sites and orchestration progenitors commit appropriate lineages arrange into patterned tissues. We identified a zinc finger transcription factor, Smed-ZicA, as downstream target Smed-FoxD, Forkhead factor required for regeneration. Smed-zicA Smed-FoxD are co-expressed with Wnt inhibitor notum Activin follistatin in cluster at anterior-most tip regenerating - anterior...
Skin is composed of diverse cell populations that cooperatively maintain homeostasis. Up-regulation the nuclear factor κB (NF-κB) pathway may lead to development chronic inflammatory disorders skin, but its role during early events remains unclear. Through analysis single-cell RNA sequencing data via iterative random forest leave one out prediction, an explainable artificial intelligence method, we identified immunoregulatory for a unique paired related homeobox-1 (Prx1) + fibroblast...
We have previously reported that preconditioning of bone marrow-derived mesenchymal stem cells (MSCs) with diazoxide (DZ) significantly improved cell survival via NF-κB signaling. Since micro-RNAs (miRNAs) are critical regulators a wide variety biological events, including apoptosis, proliferation, and differentiation, it is likely DZ-induced mediated by miRNAs. Here we show miR-146a expressed during DZ key regulator survival. Treatment MSCs (200 μM) markedly increased expression promoted...
UNC-45 is a chaperone that facilitates folding of myosin motor domains. We have used Drosophila melanogaster to investigate the role in muscle development and function. (dUNC-45) expressed at all developmental stages. It colocalizes with non-muscle embryonic blastoderm 2-hour-old embryos. At 14 hours, it accumulates most strongly striated muscles, similarly myosin. dUNC-45 localizes Z-discs sarcomeres third instar larval body-wall muscles. produced dunc-45 mutant which zygotic expression...
Two T lymphocyte-specific antisera, i.e. naturally-occurring auto-antibody to cells of systemic lupus erythematosus patients (natural cell toxic autoantibody) and heterologous antiserum against human brain tissue (antibrain-associated T-cell antigen), were used detect surface antigens peripheral lymphocytes. Nylon column-purified from normal aged individuals with Werner's syndrome (a premature aging syndrome) reacted these auto- antibodies followed by staining appropriate fluorescence...
The immunomodulating effects of various gel-forming (1→3)-β-glucans, grifolan (GRN), SSG, sonifilan (SPG) and alkaline-treated SPG (SPG-OH), on balancing helper T cell activity were examined in a murine model. Plasma from mice that injected with GRN or SPG-OH trinitrophenyl ovalbumin (TNP-OVA) contained TNP-specific antibodies both IgG1 IgG2a isotypes. Administration SSG TNP-OVA significantly augmented the synthesis antibodies, while was reduced. However, did not enhance antibody response....
During animal development, cells undergo dynamic changes in position and gene expression. A collection of quantitative information about morphological dynamics under a wide variety perturbations would provide rich resource for understanding the molecular mechanisms development. Here, we created database, Worm Developmental Dynamics Database (http://so.qbic.riken.jp/wddd/), which stores cell division early Caenorhabditis elegans embryos with single genes silenced by RNA-mediated interference....
Magnetic resonance imaging with high static magnetic fields (SMFs) has become widely used for medical purposes because SMFs cause fewer genotoxic side effects than ionizing radiation (IR). However, the effect of exposure to on global transcription is little understood. We demonstrate that genes involved in motor activity, actin binding, cell adhesion, and cuticles are transiently specifically induced following 3 or 5 T SMF experimental model metazoan Caenorhabditis elegans. In addition,...
Systemic lupus erythematosus (SLE) is an autoimmune disease with multiple immune disturbances whose mechanisms remain unclear. We examined the interaction of antilymphocyte antibodies cultured normal T lymphocytes. cells were prepared by E-rosetting after petri-dish removal adherent and for 2-7 d in presence SLE sera or human sera. Cultured washed sonicated, amount cell-associated IgG was quantitated radioimmunoassay enzyme-linked immunoassay (ELISA) methods. 27 39 showed marked increments...
Human red cells sensitized with one of the Rh antisera (Ripley) form rosettes (Fc-rosette) human B lymphocytes and are well inhibited by aggregated IgG. Since sera hypocomplementaemic patients systemic lupus erythematosus (SLE) have frequently been reported to contain immune complexes, they were used for inhibition Fc-rosette formation in this study. The results rates inversely correlated serum CH50 levels. When separated into top, middle, bottom fractions ultracentrifugation, showed more...
Differentiation of oligodendroglial progenitor cells (OPCs) into myelinating oligodendrocytes is known to be regulated by the microenvironment where they differentiate. However, current research has not verified whether or lineage (OLCs) derived from different anatomical regions central nervous system (CNS) respond microenvironmental cues in same manner. Here, we isolated pure OPCs rat neonatal forebrain (FB) and spinal cord (SC) compared their phenotypes vitro conditions. We found that...
Concanavalin A (Con A) froms precipitates with carbohydrate-rich protein such as IgM, IgD, IgE, and IgA. Since IgG contains little carbohydrate does not react Con A, the activity of IgG-rheumatoid factor (RF) can be measured in supernate A-treated serum. When latex fixation test (LFT) sensitized sheep cell agglutination (SSCA) were perfromed for detection IgG-RF, LFT was positive 32-1% sera, out 137 sera originally LFT, SSCA 18-5% 119 SSCA. IgG-RF exhibited lower complement fixing ability...
Objectives/Hypothesis Individuals with orofacial clefts often experience respiratory problems because of nasopharyngeal abnormalities. Pharyngeal airway morphology is thought to differ among the various cleft types. We measured three‐dimensional (3D) volume using cone‐beam computed tomography (CBCT) analysis evaluate and compare pharyngeal airways in Japanese preschoolers without clefts. Study Design Retrospective case‐control study. Methods enrolled 83 subjects (37 boys, 46 girls; mean age...
Natural killing (NK) activities were measured in peripheral blood lymphocytes (PBL) from 14 patients with Werner's syndrome (WS), and the results compared those of 187 normal individuals at different ages. The NK healthy donors demonstrated a characteristic invariable manner accordance age sex, whereas WS considerably reduced irrespective sex. When PBL preincubated sera containing anti-lymphocyte antibodies (ALA) plus rabbit complement, surviving markedly when treated other heterologous ALA...