A5021421301- Spinal Cord Injury Research
- Neuroscience of respiration and sleep
- Zebrafish Biomedical Research Applications
- Nerve Injury and Rehabilitation
- Stroke Rehabilitation and Recovery
- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Spinal Dysraphism and Malformations
- Traumatic Brain Injury Research
- Cardiac Arrest and Resuscitation
- Muscle activation and electromyography studies
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Cerebral Palsy and Movement Disorders
- Veterinary Orthopedics and Neurology
- Mesenchymal stem cell research
- Neonatal and fetal brain pathology
- Neurogenetic and Muscular Disorders Research
- Transcranial Magnetic Stimulation Studies
- Cervical and Thoracic Myelopathy
- Epigenetics and DNA Methylation
- Heart Rate Variability and Autonomic Control
- HIV Research and Treatment
- Pluripotent Stem Cells Research
University of Louisville
2016-2025
Neurological Surgery
2016-2025
University of Louisville Hospital
2010-2024
Titan Pharmaceuticals (United States)
2004
Oncology Specialists
2004
Institute of Neurobiology
2002
University of Manitoba
1995-1997
Children's National
1997
University of Ottawa
1993-1995
University College London
1990-1991
Tat is an 86- to 104-amino-acid viral protein that activates human immunodeficiency virus type 1 expression, modifies several cellular functions, and causes neurotoxicity. Here, we determined the extent which peptide fragments of BRU Tat1-86 produced neurotoxicity, increased levels intracellular calcium ([Ca2+]i), affected neuronal excitability. Tat31-61 but not Tat48-85 dose dependently cytotoxicity [Ca2+]i in cultured fetal brain cells. Similarly, depolarized rat hippocampal CA1 neurons...
Abstract The human immunodeficiency virus type 1 (HIV‐1) protein Tat is known to be released from HIV‐1‐infected cells. We show that micromolar concentrations of depolarized young rat and adult neurons. In addition, Tat, at similar concentrations, was toxic fetal neurons in culture. Tat‐induced responses were insensitive the Na + channel blocker tetrodotoxin, suggesting a direct effect on depolarizations cytotoxicity blocked by excitatory amino acid antagonist kynurenate. N ‐methylv D...
Demyelination contributes to the dysfunction after traumatic spinal cord injury (SCI). We explored whether combination of neurotrophic factors and transplantation adult rat oligodendrocyte precursor cells (OPCs) could enhance remyelination functional recovery SCI. Ciliary factor (CNTF) was most effective promote (OL) differentiation survival OPCs in vitro . were infected with retroviruses expressing enhanced green fluorescent protein (EGFP) or CNTF transplanted into contused thoracic 9 d...
Identification of long tracts responsible for the initiation spontaneous locomotion is critical spinal cord injury (SCI) repair strategies. Pathways derived from mesencephalic locomotor region and pontomedullary medial reticular formation fictive in decerebrate preparations project to thoracolumbar levels via reticulospinal axons ventrolateral funiculus (VLF). However, white matter regions over-ground remain unclear because cats, monkeys, humans display varying degrees recovery after ventral...
The effects of specific mitogens and substrates on the proliferative capacity differentiated phenotypic plasticity neural precursor cell populations isolated from adult rat subventricular zone (SVZ) were examined. SVZ cells grown uncoated tissue culture plastic, extracellular matrix, or poly-d-ornithine with either laminin fibronectin. could not be generated platelet-derived growth factor (PDGF), granulocyte macrophage colony stimulating factor, stem heparin-binding epidermal (HB-EGF),...
Advances in spinal cord injury (SCI) research are dependent on quality animal models, which turn rely sensitive outcome measures able to detect functional differences animals following injury. To date, most measurements of dysfunction SCI either the subjective rating observers or slow throughput manual gait assessment. The present study compares normal and contusion-injured mice using TreadScan system. utilizes a transparent treadmill belt high-speed camera capture footprints automatically...
Our understanding of the substrates locomotion, and hence our causes deficits following spinal cord injury, is still incomplete. While severe locomotor can be induced by either contusion or laceration injuries demyelination thoracic ventral ventrolateral white matter, loss mid-thoracic gray matter (intraspinal kainic acid injection) has no impact on locomotion. In contrast, from rostral lumbar segments induces deficits. This study examines histological outcomes involving enlargement in adult...
One of the most promising rehabilitation strategies for spinal cord injury is weight-supported treadmill training. This strategy seeks to re-train below level generate a meaningful pattern movement. However, number step cycles that can be accomplished limited by poor weight-bearing capability neuromuscular system after injury. We have begun study swimming as allows high numbers steps and step-cycle frequency in standard rat model contusive The purpose present was evaluate effect rats with...
The majority of animal studies examining the recovery function following spinal cord injury use BBB Open-Field Locomotor Scale as a primary outcome measure. However, it is now well known that rehabilitation strategies can bring about significant improvements in hindlimb some models. Thus, walking rats may be influenced by differences activity levels and housing conditions during first few weeks post-injury. Swimming natural form locomotion animals are not normally exposed to laboratory...
Background. The authors have shown that rats can be retrained to swim after a moderately severe thoracic spinal cord contusion. They also found improvements in body position and hindlimb activity occurred rapidly over the first 2 weeks of training, reaching plateau by week 4. Overground walking was not influenced suggesting swimming may task-specific model locomotor retraining. Objective. To provide quantitative description movements uninjured adult during swimming, then injury Methods. used...
To study the pathophysiology of spinal cord injury (SCI), we used LISA-Vibraknife to generate a precise and reproducible dorsal laceration SCI in mouse. The surgical procedure involved T9 laminectomy, dural resection, precisely controlled depth. Four hemisection injuries with lesion depths 0.5, 0.8, 1.1, 1.4 mm, as well normal, sham (laminectomy removal only), transection controls were examined. Assessments including Basso Mouse Scale (BMS), footprint analysis, beam walk, toe spread reflex,...
Background. Locomotor training of rats with thoracic contusion spinal cord injuries can induce task-specific changes in stepping but rarely results improved overground locomotion, possibly due to a ceiling effect. Thus, the authors hypothesize that incompletely injured maximally retrain themselves while moving about their cages over first few weeks postinjury. Objective. To test hypothesis using hindlimb immobilization after mild injury adult female rats. A passive stretch protocol was...
Efforts to understand spinal cord injury (SCI) and other complex neurotrauma disorders at the pre-clinical level have shown progress in recent years. However, successful translation of basic research into clinical practice has been slow, partly because large, heterogeneous data sets involved. In this sense, translational neurological represents a "big data" problem. an effort expedite knowledge standards patient care for SCI, we describe development novel database known as Visualized...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis, resolution, and absolute configuration of the isomers neuronal excitant 1-amino-1,3-cyclopentanedicarboxylic acidKenneth Curry, M. J. Peet, D. S. K. Magnuson, H. McLennanCite this: Med. Chem. 1988, 31, 4, 864–867Publication Date (Print):April 1, 1988Publication History Published online1 May 2002Published inissue 1 April 1988https://pubs.acs.org/doi/10.1021/jm00399a030https://doi.org/10.1021/jm00399a030research-articleACS...