A5032644073- Inflammatory mediators and NSAID effects
- Genomics, phytochemicals, and oxidative stress
- Skin Protection and Aging
- Cancer Immunotherapy and Biomarkers
- Cancer, Stress, Anesthesia, and Immune Response
- Peroxisome Proliferator-Activated Receptors
- Bioactive Compounds and Antitumor Agents
- NF-κB Signaling Pathways
- Photodynamic Therapy Research Studies
- Dermatology and Skin Diseases
- Inflammatory Biomarkers in Disease Prognosis
- Asthma and respiratory diseases
- Pancreatic and Hepatic Oncology Research
- PI3K/AKT/mTOR signaling in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Extracellular vesicles in disease
- Inflammasome and immune disorders
- Eicosanoids and Hypertension Pharmacology
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Synthesis and biological activity
- Immunotherapy and Immune Responses
- Burn Injury Management and Outcomes
- Immune Response and Inflammation
Wright State University
2016-2025
Chulabhorn Graduate Institute
2025
Indian Institute of Technology Kanpur
2022
Indiana University – Purdue University Indianapolis
2010-2019
Indiana University School of Medicine
2010-2019
Dayton Clinical Oncology Program
2018
All India Institute of Medical Sciences Bhubaneswar
2018
Zagazig University
2017
Indiana University
2014-2015
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2005-2014
Curcumin has been shown to inhibit the growth of various types cancer cells; however, at concentrations much above clinically achievable levels in humans. The concentration curcumin achieved plasma after oral administration humans was estimated be around 1.8 microM. Here, we report that treatment BxPC-3 human pancreatic cells with a low and single exposure 2.5 microM for 24 h causes significant arrest G2/M phase induces apoptosis. Immunoblot studies revealed increased phosphorylation H2A.X...
Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, has been reported to have anticancer properties, but the mechanism whereby it inhibits growth of human pancreatic cancer cells is incompletely understood.Human (BxPC-3, AsPC-1, Capan-2, MiaPaCa-2, and Panc-1) immortalized (HPDE-6) were treated with vehicle or BITC at 5-40 microM, cell survival was evaluated by sulforhodamine B assay, apoptosis caspase-3 poly-ADP ribose polymerase cleavage commercial assay for death....
In our previous studies, we have shown that benzyl isothiocyanate (BITC) inhibits the growth of human pancreatic cancer cells by inducing apoptosis. present study, demonstrate activation all three (MAPK) family members [extracellular signal-regulated protein kinase (ERK), c-jun N-terminal (JNK) and P38] in response to BITC treatment. Exposure Capan-2 with varying concentrations for 24 h resulted phosphorylation (activation) ERK at Thr202/Tyr204, JNK Thr183/Tyr185 P38 Thr180/Tyr182, leading...
Our previous studies have shown that benzyl isothiocyanate (BITC) suppresses pancreatic tumor growth by inhibiting STAT-3; however, the exact mechanism of suppression was not clear. Here we evaluated effects and BITC on angiogenesis. results reveal significantly inhibits neovasularization rat aorta Chicken-Chorioallantoic membrane. Furthermore, blocks migration invasion BxPC-3 PanC-1 cancer cells in a dose dependant manner. Moreover, secretion VEGF MMP-2 normoxic hypoxic suppressed BITC....
A complete carcinogen, ultraviolet B (UVB) radiation (290–320 nm), is the major cause of skin cancer. UVB-induced systemic immunosuppression that contributes to photocarcinogenesis due glycerophosphocholine-derived lipid mediator platelet-activating factor (PAF). question in photobiology how UVB radiation, which only absorbs appreciably epidermal layers skin, can generate effects. exposure and PAF receptor (PAFR) activation keratinocytes induce release large numbers microvesicle particles...
Abstract Background Triphala is commonly used in Ayurvedic medicine to treat variety of diseases; however its mechanism action remains unexplored. This study elucidates the molecular against human pancreatic cancer cellular and vivo model. Methods Growth-inhibitory effects were evaluated Capan-2, BxPC-3 HPDE-6 cells by Sulphoradamine-B assay. Apoptosis was determined cell death assay western blotting. administered orally nude mice implanted with Capan-2 xenograft. Tumors analyzed...
NF-kappaB/p65 is constitutively activated in pancreatic cancers, where it plays a critical role the transcriptional activation of multiple cell survival genes. We have previously shown apoptosis-inducing effects benzyl isothiocyanate (BITC) cancer cells. hypothesized that inhibition could be mechanism BITC-induced apoptosis. Therefore, effect BITC on was evaluated BxPC-3, Capan-2, and normal HPDE-6 cells by Western blotting, DNA-binding activity, immunohistochemistry xenografted tumors. Our...
Ubiquitous pro-oxidative stressor ultraviolet B radiation (UVB) to human or mouse skin generates platelet-activating factor (PAF) and novel oxidatively modified glycerophosphocholines (Ox-GPCs) with PAF-receptor (PAF-R) agonistic activity. These lipids mediate systemic immunosuppression in a process involving IL-10. The current studies sought determine the functional significance of UVB-mediated an established model murine melanoma. We show that UVB irradiation augments B16F10 tumor growth...
Pumpkin seed oil has long been considered as an ingredient for its nutritional and medicinal values the prevention of various ailments, especially prostate diseases. In addition, several studies have suggested crucial roles effectiveness pumpkin in treatment diabetes, anxiety even cancer. is being used countries worldwide including North America, Mexico, India China. This review highlights characterization, properties use from species against diseases pathophysiologies. We strongly believe...
Abstract Previous studies have established that pro-oxidative stressors suppress host immunity because of their ability to generate oxidized lipids with platelet-activating factor receptor (PAF-R) agonist activity. Although exposure the stressor cigarette smoke (CS) is known exert immunomodulatory effects, little regarding role PAF in these events. The current sought determine PAF-R signaling CS-mediated effects. We demonstrate CS induces generation a transient agonistic activity blood mice....
Oxidative stress suppresses host immunity by generating oxidized lipid agonists of the platelet-activating factor receptor (PAF-R). Because many classical chemotherapeutic drugs induce reactive oxygen species (ROS), we investigated whether these might subvert activating PAF-R. Here, show that PAF-R are produced in melanoma cells chemotherapy is administered vitro, vivo, or human subjects. Structural characterization induced revealed multiple glycerophosphocholines generated nonenzymatically....
// Ravi P. Sahu 1 , Kathleen A. Harrison 2 Jonathan Weyerbacher 3 Robert C. Murphy Raymond L. Konger 4 Joy Elizabeth Garrett 5 Helen Jan Chin-Sinex Michael Edward Johnston II Joseph R. Dynlacht Marc Mendonca Kevin McMullen Gengxin Li 6 Dan F. Spandau Jeffrey B. Travers 1, 3, 7 Department of Pharmacology and Toxicology, Boonshoft School Medicine at Wright State University, Dayton, OH, USA Pharmacology, University Colorado Health Sciences Center, Aurora, CO, Dermatology, Indiana Medicine,...