A5101907574- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Intracerebral and Subarachnoid Hemorrhage Research
- Cardiovascular Disease and Adiposity
- Renin-Angiotensin System Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
- Traumatic Brain Injury and Neurovascular Disturbances
- Ferroptosis and cancer prognosis
- Inflammasome and immune disorders
- Neurological Disease Mechanisms and Treatments
- Skin Protection and Aging
- Adipose Tissue and Metabolism
- S100 Proteins and Annexins
- Immune cells in cancer
- Hydrogen's biological and therapeutic effects
- Inflammatory Biomarkers in Disease Prognosis
- Barrier Structure and Function Studies
- Bacterial Infections and Vaccines
- Receptor Mechanisms and Signaling
- Atherosclerosis and Cardiovascular Diseases
- Acute Ischemic Stroke Management
- Biomarkers in Disease Mechanisms
Marshall University
2020-2025
Wright State University
2014-2024
Huntington University
2023
First Affiliated Hospital of Harbin Medical University
2020
ORCID
2020
Harbin Medical University
2020
Third Xiangya Hospital
2020
Central South University
2020
Dayton Clinical Oncology Program
2020
Guangdong Medical College
2015-2016
Glioma stem cells (GSCs) play an important role in glioblastoma prognosis. Exosomes (EXs) mediate cell communication by delivering microRNAs (miRs). Glioblastoma has a high level of miR-21 which could upregulate vascular endothelial growth factor (VEGF) expression. We hypothesized GSC-EXs can promote the angiogenic ability (ECs) through miR-21/VEGF signal. GSCs were isolated from U-251 with marker CD133. transfected without or scramble mimics used to produce GSC-EXscon, GSC-EXssc and...
Angiotensin-converting enzyme 2 (ACE2) is an emerging cardiovascular protective target that mediates the metabolism of angiotensin (Ang) II into Ang (1–7). Our group has demonstrated ACE2 overexpression enhances function endothelial progenitor cells (EPCs). Here, we investigated whether ACE2-primed EPCs (ACE2-EPCs) can protect cerebral microvascular (ECs) against injury and dysfunction in vitro model, with focusing on their exosomal cytokine paracrine effects mitochondria. Human were...
Abstract Backgrounds/aims Mesenchymal stromal cell-derived exosomes (MSC-EXs) could exert protective effects on recipient cells by transferring the contained microRNAs (miRs), and miR-132-3p is one of angiogenic miRs. However, whether combination MSC-EXs has better in ischemic cerebrovascular disease remains unknown. Methods Mouse MSCs transfected with scrambler control or mimics were used to generate miR-132-3p-overexpressed (MSC-EXs ). The EXs hypoxia/reoxygenation (H/R)-injured ECs ROS...
Exercise has cardiovascular benefits which might be related to endothelial progenitor cells (EPC). Meanwhile, there is evidence suggesting that EPC-derived exosomes (EPC-EX) promote vascular repair and angiogenesis through their carried microRNA (miR)-126. In this study, we investigated whether exercise could increase the levels of circulating EPC-EX miR-126 cargo, by protective function on (EC).Plasma from sedentary, low, or moderate mice, respectively, denoted as EPC-EX, were isolated...
Endothelial cell (EC) released microvesicles (EMVs) can affect various target cells by transferring carried genetic information. Astrocytes are the main components of blood brain barrier (BBB) structure in and participate regulating BBB integrity flow. The interactions between ECs astrocytes essential for homeostasis pathological conditions. Here, we studied effects human microvascular EMVs on astrocyte functions. Additionally, investigated treated function cerebral ischemic damage.EMVs...
Abstract Aims We previously showed that the protective effects of endothelial progenitor cells (EPCs)‐released exosomes (EPC‐EXs) on endothelium in diabetes. However, whether EPC‐EXs are diabetic ischemic stroke is unknown. Here, we investigated mice and tested miR‐126 enriched (EPC‐EXs miR126 ) have enhanced efficacy. Methods The db/db subjected to were intravenously administrated with 2 hours after stroke. infarct volume, cerebral microvascular density (MVD), blood flow (CBF), neurological...
Abstract Background We have previously verified the beneficial effects of exosomes from endothelial progenitor cells (EPC-EXs) in ischemic stroke. However, EPC-EXs hemorrhagic stroke not been investigated. Additionally, miR-137 is reported to regulate ferroptosis and be involved neuroprotection against Hence, present work explored miR-137-overexpressing on apoptosis, mitochondrial dysfunction, oxyhemoglobin (oxyHb)-injured SH-SY5Y cells. Methods The lentiviral was transfected into EPCs then...
Stem cell-derived exosomes (EXs) offer protective effects on various cells via their carried microRNAs (miRs). Meanwhile, miR-210 has been shown to reduce mitochondrial reactive oxygen species (ROS) overproduction. In this study, we determined the potential of endothelial progenitor EXs (EPC-EXs) hypoxia/ reoxygenation (H/R) injured (ECs) and investigated whether these could be boosted by loading.Human EPCs were used generate EPC-EXs, or transfected with scrambler control mimics EPC-EXssc...
Abstract Oxidative stress is one of the mechanisms ageing‐associated vascular dysfunction. Angiotensin‐converting enzyme 2 (ACE2) and microRNA (miR)‐18a have shown to be down‐regulated in ageing cells. Our previous study has that ACE2‐primed endothelial progenitor cells (ACE2‐EPCs) protective effects on (ECs), which might due their released exosomes (EXs). Here, we aimed investigate whether ACE2‐EPC‐EXs could attenuate hypoxia/reoxygenation (H/R)‐induced injury ECs through carried miR‐18a....
A complete carcinogen, ultraviolet B (UVB) radiation (290–320 nm), is the major cause of skin cancer. UVB-induced systemic immunosuppression that contributes to photocarcinogenesis due glycerophosphocholine-derived lipid mediator platelet-activating factor (PAF). question in photobiology how UVB radiation, which only absorbs appreciably epidermal layers skin, can generate effects. exposure and PAF receptor (PAFR) activation keratinocytes induce release large numbers microvesicle particles...
We have recently demonstrated that exosomal communication between endothelial progenitor cells (EPCs) and brain is compromised in hypertensive conditions, which might contribute to the poor outcomes of stroke subjects with hypertension. The present study investigated whether exercise intervention can regulate EPC–exosome (EPC-EX) functions conditions. Bone marrow EPCs from sedentary exercised transgenic mice were used for generating EPC-EXs, denoted as R-EPC-EXs R-EPC-EXET. microRNA profile...
Our previous study showed that circulating microvesicles (cMVs) of diabetic mice have negative effects on the function endothelial progenitor cells (EPCs). Whether this is true in patients deserves further study. In study, cMVs and EPC-derived MVs (EPC-MVs) EPC migration, apoptosis, reactive oxygen species (ROS) production healthy controls, well-controlled, uncontrolled were investigated. The levels miR-126 vascular growth factor receptor 2 (VEGFR2) cMVs, EPC-MVs, and/or EPCs analyzed....
Exosomes (EXs) are cell-derived vesicles that mediate cell-cell communication and could serve as biomarkers. Here we described novel methods for purification phenotyping of EXs released from endothelial cells (ECs) progenitor (EPCs) by combining microbeads fluorescence quantum dots (Q-dots®) techniques. the culture medium ECs EPCs were isolated detected with cell-specific antibody conjugated second Q-dots using nanoparticle tracking analysis (NTA) system. The sensitivities cell origin...
Protection of cerebral endothelial cells (ECs) from hypoxia/reoxygenation (H/R)-induced injury is an important strategy for treating ischemic stroke. In this study, we investigated whether co-culture with progenitor (EPCs) and neural (NPCs) synergistically protects ECs against H/R the underlying mechanism. EPCs NPCs were respectively generated inducible pluripotent stem cells. Human brain used to produce in vitro H/R-injury model. Data showed: 1) Co-culture inhibited H/R-induced reactive...
Perivascular adipose tissue (PVAT), a type of that surrounds the blood vessels, has been considered an active component vessel walls and involved in vascular homeostasis. Recent evidence shows increased inflammation oxidative stress PVAT contribute to endothelial dysfunction 2 diabetes (T2D). Exercise is important nonpharmacological approach for diseases. However, there limited information regarding whether beneficial effects exercise on function related status. In this study, we...
We have demonstrated that neural progenitor cells (NPCs) protect endothelial (ECs) from oxidative stress. Since exosomes (EXs) can convey the benefit of parent through their carried microRNAs (miRs) and miR‐210 is ubiquitously expressed with versatile functions, we investigated role in effects NPC‐EXs on stress dysfunction ECs. NPCs were transfected control scramble/inhibitor/mimic to generate con , sc anti-miR-210 miR-210 . The various angiotensin II‐ (Ang II‐) induced reactive oxygen...
Abstract Background Hyperglycemia contributes to cardiovascular complications in patients with type 2 diabetes. We confirmed that high glucose (HG) induces endothelial dysfunction and cerebral ischemic injury is enlarged diabetic mice. Stem cell-released exosomes have been shown protect the brain from stroke. previously progenitor cells (EPCs)-released (EPC-EXs) can hypoxia/reoxygenation (H/R) HG-induced injury. Here, we aim investigate effects of EPC-EXs on astrocytes under H/R whether...