A5027395359- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Estrogen and related hormone effects
- Diet and metabolism studies
- Regulation of Appetite and Obesity
- Muscle metabolism and nutrition
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Sirtuins and Resveratrol in Medicine
- Adipokines, Inflammation, and Metabolic Diseases
- Phytoestrogen effects and research
- Genomics, phytochemicals, and oxidative stress
- Antioxidant Activity and Oxidative Stress
- Cancer, Lipids, and Metabolism
- Metabolism, Diabetes, and Cancer
- Genetics, Aging, and Longevity in Model Organisms
- Fatty Acid Research and Health
- Birth, Development, and Health
- Animal Nutrition and Physiology
- Stress Responses and Cortisol
- Biochemical effects in animals
- Cancer-related Molecular Pathways
- Metabolism and Genetic Disorders
- Exercise and Physiological Responses
- Kidney Stones and Urolithiasis Treatments
Health Research Institute of the Balearic Islands
2016-2025
Universitat de les Illes Balears
2015-2024
Servei de Salut de les Illes Balears
2015-2024
Research Institute of Health Sciences
2015-2024
Instituto de Salud Carlos III
2015-2024
Centro de Investigación Biomédica en Red
2012-2024
Hospital Universitario Son Espases
2016-2024
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2011-2021
Departament de Salut
2008-2009
University of Sannio
2008
Gender-related differences in brown adipose tissue (BAT) thermogenesis of 110-day-old rats were studied by determining the morphological and functional features BAT. The adrenergic control was assessed studying levels β<sub>3</sub>- α<sub>2A</sub>-adrenergic receptors (AR) lipolytic response to norepinephrine (β<sub>1</sub>-, β<sub>2</sub>-, β<sub>3</sub>-, α<sub>2</sub>-AR agonist), isoprenaline β<sub>3</sub>-AR CGP12177A (selective partial agonist but β<sub>1</sub>- β<sub>2</sub>-AR...
Gender and diet have an important effect in cardiovascular disease other aging-associated disorders, whose initiation and/or worsening seem to be delayed females from different species animals subjected caloric restriction (CR). The aim of the present study was investigate whether cardiac muscle bioenergetic mitochondrial features could responsible for these beneficial effects.Fifteen-month-old male female Wistar rats were fed ad libitum or 40% CR 3 months. Cardiac function (citrate synthase...
The treatment of advanced colorectal cancer with 5-fluorouracil has two major problems: development tumor resistance and toxicity toward normal tissues. aim this study was to investigate the possible advantages combining (5-FU) resveratrol (<i>trans</i>-3, 4′, 5-trihydroxystilbene) for treating HT-29 SW-620 carcinoma cell lines. Since combined using 5-FU resulted in a significant decrease long-term survival, we investigated basis synergistic interaction at molecular level, focusing on...
Breast cancer is the most common malignancy in women of developed countries. The aim this study was to investigate whether genistein, a soy phytoestrogen, and 17β-estradiol (E2) could have effects on cell cycle mitochondrial function dynamics. Three human breast lines with different estrogen receptor alpha (ERα) beta (ERβ) ratio were used: MCF-7 (high ERα/ERβ ratio), T47D (low ratio) MDA-MB-231 (ER-negative). Cell proliferation, cycle, functionality, dynamics parameters analyzed. E2...
SIRT3, the major deacetylase in mitochondria, plays a crucial role modulating ROS production and scavenging by regulating key proteins implicated mitochondrial turnover antioxidant defenses. Therefore, SIRT3 could confer resistance to chemotherapy-induced oxidative stress, leading lower higher cell survival. Our aim was analyze whether silencing breast cancer cells through specific siRNA increase stress thus compromise response, resulting sensitization of cisplatin (CDDP) or tamoxifen (TAM)....
Sirtuin 3 (SIRT3) is the main mitochondrial deacetylase and targets several crucial enzymes against oxidative stress. Recent reports suggest that SIRT3 could also participate in quality quantity control of mitochondria. The aim this study was to analyze whether silencing colon cancer cells affect biogenesis impair function. For purpose, metastatic cell line SW620 transfected with a specific shRNA obtain stable knockdown. Gene expression protein levels proteins related function were...
Previous studies suggest that sex hormones could be responsible, at least in part, for the gender-dependent thermogenesis found adrenergic control of brown adipose tissue (BAT) under conditions and response to diet cold. Catecholamines, as well several hormones, including may alter function or expression different adrenoceptor subtypes adipocytes vivo, a confirmation provided by vitro experiments. Therefore, effect testosterone, 17 beta-estradiol, progesterone, norepinephrine (NE) on...
Control of mitochondrial biogenesis in brown adipose tissue (BAT), as part the thermogenesis program, is a complex process that requires integration multiple transcription factors to orchestrate and nuclear gene expression. Despite knowledge role sex hormones on BAT physiology, little known about effect these biogenic program. The aim this study was determine testosterone, 17beta-estradiol, progesterone expression involved control thermogenic function such ppargamma, pgc1alpha, nrf1, gabpa,...
The peptide apelin has been located in a wide range of tissues, including the gastrointestinal tract, stomach and adipose tissue. Apelin its receptor also detected arcuate paraventricular nuclei hypothalamus, which are involved control feeding behaviour energy expenditure. This distribution suggests may play role homeostasis, but previous attempts to discern effects by acute injection into brain have yielded conflicting results. We examined effect chronic 10‐day intracerebroventricular...
Caloric restriction (CR) without malnutrition has been shown to increase maximal life span and delay the rate of aging in a wide range species. It proposed that reduction energy expenditure oxidative damage may explain life-extending effect CR. Sex-related differences also have influence longevity many mammalian The aim present study was determine sex-related rat liver mitochondrial machinery, bioenergetics, balance response short-term Mitochondria were isolated from 6-mo-old male female...
Recent investigations suggest that sex hormones play an important role in the brown adipose tissue (BAT) thermogenic program by acting on several steps of lipolytic signal cascade and UCP1 transcription control. However, number studies focusing steroid receptor status is negligible. In present study, we analyze mRNA levels male female rats pregnant lactating females, all them models with a different hormonal background. The direct effect expression their receptors was studied vitro primary...
Sex-related differences in energy balance were studied young Wistar rats fed standard chow pellets either ad libitum or restricted amounts (60% of intake) for 100 days. Caloric intake, indirect calorimetry, organ and adipose tissue weights, efficiency, liver mitochondrial respiration rate, brown (BAT) uncoupling protein-1 (UCP1) content measured. Ad libitum-fed females showed greater oxygen consumption (Vo(2)) carbon dioxide production (Vco(2)) lower efficiency than males. restriction...
The effect of gender and caloric restriction on mitochondrial content oxidative-phosphorylative capacities has been investigated in rat gastrocnemius muscle. Muscle protein, protein DNA contents, enzymatic activities oxidative phosphorylative system, antioxidant enzymes, levels complex IV (subunit I IV) ATPase, the gene expression transcription factor A (TFAM), involved replication transcription, were measured rats both genders fed ad libitum subjected to three months 40% restriction....