A5101908876- Catalytic C–H Functionalization Methods
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Asymmetric Hydrogenation and Catalysis
- Synthesis and Catalytic Reactions
- Catalytic Cross-Coupling Reactions
- Biofuel production and bioconversion
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Plant-Microbe Interactions and Immunity
- DNA and Nucleic Acid Chemistry
- Enzyme Production and Characterization
- Gut microbiota and health
- RNA Interference and Gene Delivery
- Fungal and yeast genetics research
- Chemical synthesis and alkaloids
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Asymmetric Synthesis and Catalysis
- Fluorine in Organic Chemistry
- Cyclopropane Reaction Mechanisms
- Microbial Metabolism and Applications
- Monoclonal and Polyclonal Antibodies Research
- Plant Gene Expression Analysis
- Synthesis and pharmacology of benzodiazepine derivatives
Zhejiang University
2022-2024
Nanjing University of Chinese Medicine
2021-2024
Massachusetts Institute of Technology
2019-2024
Anhui Medical University
2023-2024
Jiangsu Normal University
2020-2024
Second Hospital of Anhui Medical University
2024
Zhejiang University of Science and Technology
2023
The Synergetic Innovation Center for Advanced Materials
2023
Suzhou Municipal Hospital
2023
South China University of Technology
2022
Chiral tertiary alcohols are important building blocks for the synthesis of pharmaceutical agents and biologically active natural products. The addition carbon nucleophiles to ketones is most common approach alcohol but traditionally relies on stoichiometric organometallic reagents that difficult prepare, sensitive, uneconomical. We describe a mild efficient method copper-catalyzed allylation using widely available 1,3-dienes as allylmetal surrogates. Homoallylic bearing wide range...
An efficient method for sterically demanding Suzuki–Miyaura coupling reactions has been developed with two catalysts, Pd/BI-DIME (see scheme) and Pd/phenanthrene-based ligand. The catalyst facilitates the syntheses of extremely hindered biaryls bearing ortho-isopropyl substituents. other is synthesis functionalized tetra-ortho-substituted at low loadings. As a service to our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed...
Abstract Recently, CuH‐catalyzed reductive coupling processes involving carbonyl compounds and imines have become attractive alternatives to traditional methods for stereoselective addition because of their ability use readily accessible stable olefins as surrogates organometallic nucleophiles. However, the inability aldehydes, which usually reduce too rapidly in presence copper hydride complexes be viable substrates, has been a major limitation. Shown here is that by exploiting relative...
The intestinal mucus barrier is an important line of defense against gut pathogens. Damage to this brings bacteria into close contact with the epithelium, leading inflammation. Therefore, its restoration a promising strategy for alleviating This study showed that
Molecular dynamics simulation is a powerful tool for characterizing the solution structural ensembles of cyclic peptides. However, ability to recapitulate experimental results and make accurate predictions largely depends on force fields used. In our work here, we evaluate performance seven state-of-the-art in recapitulating NMR water 12 benchmark peptides, consisting 6 pentapeptides, 4 hexapeptides, 2 heptapeptides. The show that RSFF2+TIP3P, RSFF2C+TIP3P, Amber14SB+TIP3P exhibit similar...
Abstract Bulky P,PO ligands were designed to inhibit isomerization and reduction side reactions during the cross coupling between sterically hindered aryl halides alkylboronic acids. Suzuki–Miyaura cross‐couplings di‐ ortho ‐substituted bromides acyclic secondary acids have been achieved with high yields. The method has also enabled preparation of ‐alkoxy arenes bearing isopropyl groups in excellent utility synthetic demonstrated a late‐stage modification estrone application new route...
Abstract Rapid development of antisense therapies can enable on-demand responses to new viral pathogens and make personalized medicine for genetic diseases practical. Antisense phosphorodiamidate morpholino oligomers (PMOs) are promising candidates fill such a role, but their challenging synthesis limits widespread application. To rapidly prototype potential PMO drug candidates, we report fully automated flow-based oligonucleotide synthesizer. Our optimized platform reduces coupling times by...
The utilization of diphenyl sulfoxides as versatile electrophilic coupling partners for the Suzuki–Miyaura reaction via C–S bond cleavage was successfully developed under palladium-N-heterocyclic carbene catalysis. reactions showed good functional group compatibility, proceeded well mild conditions, and provided biaryls in yields up to 96%. A wide range useful groups, such fluoro, chloro, ether, hydroxyl, amide, cyano, keto, trimethylsilyl (TMS), ester were tolerated conditions; however, use...
Application of raw starch-degrading enzymes (RSDEs) in starch processing for biofuel production can effectively reduce energy consumption and costs. RSDEs are generally produced by filamentous fungi, such as Penicillium oxalicum, but with very low yields, which seriously hampers industrialization processing. Breeding assisted random mutagenesis is an efficient way to improve fungal enzyme production.A total 3532 P. oxalicum colonies were generated after multiple rounds mutagenesis,...
This work reports an automated affinity selection-mass spectrometry (AS-MS) approach amenable to both de novo peptide binder discovery and maturation of known binders in a high-throughput selective manner.
Efficient sterically demanding aryl–alkyl Suzuki–Miyaura coupling between di-<italic>ortho</italic>-substituted aryl halides and secondary alkylboronic acids has been achieved with a Pd-AntPhos catalyst.
Abstract Species from the genus Talaromyces produce useful biomass-degrading enzymes and secondary metabolites. However, these metabolites are still poorly understood have not been explored in depth because of a lack comprehensive genetic information. Here, we report 36.51-megabase genome assembly pinophilus strain 1–95, with coverage nine scaffolds eight chromosomes telomeric repeats at their ends circular mitochondrial DNA. In total, 13,472 protein-coding genes were predicted. Of these,...
Soil fungi produce a wide range of chemical compounds and enzymes with potential for applications in medicine biotechnology. Cellular processes soil are highly dependent on the regulation under environmentally induced stress, but most underlying mechanisms remain unclear. Previous work identified key GATA-type transcription factor, Penicillium oxalicum NsdD (PoxNsdD; also called POX08415), that regulates expression cellulase xylanase genes P. PoxNsdD shares 57 to 64% identity activator NsdD,...
Abstract Bulky P,PO ligands were designed to inhibit isomerization and reduction side reactions during the cross coupling between sterically hindered aryl halides alkylboronic acids. Suzuki–Miyaura cross‐couplings di‐ ortho ‐substituted bromides acyclic secondary acids have been achieved with high yields. The method has also enabled preparation of ‐alkoxy arenes bearing isopropyl groups in excellent utility synthetic demonstrated a late‐stage modification estrone application new route...
Antisense peptide nucleic acids (PNAs) have yet to translate the clinic because of poor cellular uptake, limited solubility, and rapid elimination. Cell-penetrating peptides (CPPs) covalently attached PNAs may facilitate clinical development by improving uptake into cells. We report an efficient technology that utilizes a fully automated fast-flow instrument manufacture CPP-conjugated (PPNAs) in single shot. The machine is rapid, with each amide bond being formed 10 s. Anti-IVS2-654 PPNA...
ipso -Defluorinative amination-triggered 1,3-diamination of (trifluoromethyl)alkenes with different amines affords various monofluoroalkenylated acyclic 1,3-diamines in high yields excellent Z / E selectivity.
The spatial structure of chromatin plays important roles in normal cell functions and the regulation gene expression. three-dimensional (3D) architectures genomes many mammals plants have been elucidated, but corresponding studies on filamentous fungi, which play vital as decomposers organic matter soil, are very limited. Penicillium oxalicum is one predominant cellulolytic aerobic fungi subtropical tropical forest soils can secrete integrative cellulase xylanase under integrated regulatory...
Rapid discovery and development of serum-stable, selective, high affinity peptide-based binders to protein targets are challenging. Angiotensin converting enzyme 2 (ACE2) has recently been identified as a cardiovascular disease biomarker the primary receptor utilized by severe acute respiratory syndrome coronavirus 2. In this study, we report peptidomimetic ACE2 via selection-mass spectrometry (AS-MS). Multiple ACE2-binding peptides (ABP) were selection from canonical noncanonical libraries...