A5043916901- Planetary Science and Exploration
- Mass Spectrometry Techniques and Applications
- Spectroscopy and Laser Applications
- Atmospheric Ozone and Climate
- Astro and Planetary Science
- Space Exploration and Technology
- Spectroscopy and Quantum Chemical Studies
- Isotope Analysis in Ecology
- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Physics Studies
- Various Chemistry Research Topics
- Conservation, Biodiversity, and Resource Management
- Advanced Proteomics Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Microfluidic and Capillary Electrophoresis Applications
- Laser-Ablation Synthesis of Nanoparticles
- Biosensors and Analytical Detection
- Laser-induced spectroscopy and plasma
- Distributed systems and fault tolerance
- Cassava research and cyanide
- Carbon Nanotubes in Composites
- Rocket and propulsion systems research
- Neuroscience and Neuropharmacology Research
- Photochemistry and Electron Transfer Studies
- Ion-surface interactions and analysis
Texas State University System
2020
Jacobs (United States)
2019
Universities Space Research Association
2017-2018
Ionwerks (United States)
2010-2017
Rice University
2005-2017
Louisiana State University
2011
United States Department of the Army
2011
National Institute on Drug Abuse
2011
National Institutes of Health
2011
Baylor University
2001-2009
Mass spectrometry imaging (MSI) of tissue implanted with silver nanoparticulate (AgNP) matrix generates reproducible lipids in rodent models disease and injury. Gas-phase production acceleration size-selected 8 nm AgNP is followed by controlled ion beam rastering soft landing implantation 500 eV into tissue. Focused 337 laser desorption produces high quality images for most lipid classes rat brain (in positive mode: galactoceramides, diacylglycerols, ceramides, phosphatidylcholines,...
The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis biomolecules in complex mixtures (such as tissue sections cellular extracts), isobaric lipid, peptide, oligonucleotide molecular ions are pre-separated cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class glycosphingolipids that has different degrees sialylation. Both GD1a GD1b, structural isomers, were studied see effects on...
Ion mobility separation of two lipids prior to MALDI imaging.
We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis DNA sequencing. To fully the advantages PME, a fluorescent dye set has been developed whose absorption maxima span virtually entire visible spectrum. Unlike emission wavelength-dependent approaches identifying species, removal spectral component PME confers number including higher normalized signals from all dyes...
Protein domains involved in receptor heteromer formation are disordered and rich the amino acids necessary for of noncovalent complexes (NCX). We present mass spectral NCX data from proteins protein receptors’ epitopes obtained by combining ion mobility (IM) MALDI. focus on occurring between Dopamine D2 (D2R) Adenosine A2A receptors (A2AR) as well D2R α2 nicotinic (NR) receptor’s subunit. The IM yield information gas phase conformation singly charged which observed either directly MALDI or...
Phase shift cavity ring down is a technique that due to its long optical path length an ideal method detect weak absorptions. Coupling the custom fitted cryostat allows gas phase molecules be studied at cryogenic temperatures in thermally isolated vacuum chamber. A novel design described construct complete instrument. With cavities of 10⩽ℓ⩽43cm, lengths between 200m and 6km have been achieved. High vibrational overtones C–H (Δυ=5) are measured 130K (methane), 150K (ethylene), 155K (ethane)....
The spectra of the C–H stretch fundamental and overtones (Δυ = 1–6) CH3D have been recorded. Absorptions in visible were obtained with a phase shift cavity ring down technique where an optical is inside low temperature cryostat. below 12,000 cm−1 observed Fourier transform spectrophotometer. local mode harmonic frequency anharmonicity used harmonically coupled anharmonic oscillator (HCAO) model to calculate energy levels assign absorption bands particular transitions. Overtone absorptions 5...
We previously demonstrated that ammonium- or guanidinium-phosphate interactions are key to forming noncovalent complexes (NCXs) through salt bridge formation with G-protein coupled receptors (GPCR), which immersed in the cell membrane's lipids. The present work highlights MALDI ion mobility orthogonal time-of-flight mass spectrometry (MALDI IM oTOF MS) as a method determine qualitative and relative quantitative affinity of drugs form NCXs targeted GPCRs' epitopes model system using,...
Laser ablation of silver nanoparticles (NPs) was studied with laser post-ionization (LPI) time-of-flight mass spectrometry (TOF MS). Silver NPs containing ∼15 000 Ag atoms (4 nm radius) were deposited by soft landing (energy 3 eV/atom) onto indium tin oxide (ITO)/glass substrates. performed using frequency-doubled Ti:sapphire nanosecond pulsed irradiation at three different wavelengths (371, 401, and 421 nm), whereas for post-ionization, pulses from an F2 used. fluences time delay...
Our collaborative work to create and soft land ionized gas phase single crystalline metal nanoparticulate ions onto substrates was motivated by Robert Hauge’s wish produce templates for carbon nanotube carpet growth. Ionwerks created a machine do this but, as is often the case, funding found first alternative applications. One such has been low energy (500 eV) injection of 8 nm silver (AgNP - ) into near surface bio-polymers. A uniform layer (70 e15 Ag atoms/cm 2 these implanted particles...