A5089310689- Optical Coherence Tomography Applications
- Advanced biosensing and bioanalysis techniques
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Health and Medical Research Impacts
- Advanced Biosensing Techniques and Applications
- Digital Holography and Microscopy
- Nanoplatforms for cancer theranostics
- COVID-19 epidemiological studies
- Analytical Chemistry and Sensors
- Advances in Oncology and Radiotherapy
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Innovations in Medical Education
- Biomedical and Engineering Education
- Carbon Nanotubes in Composites
- Biosensors and Analytical Detection
- Hemoglobinopathies and Related Disorders
- Nanopore and Nanochannel Transport Studies
- Angiogenesis and VEGF in Cancer
- SARS-CoV-2 and COVID-19 Research
- Data-Driven Disease Surveillance
- COVID-19 Clinical Research Studies
Mount Sinai Health System
2025
Massachusetts Institute of Technology
2003-2023
Icahn School of Medicine at Mount Sinai
2020-2022
University of Illinois Urbana-Champaign
2005-2018
East Tennessee State University
2010
Rice University
2005-2010
Carle Foundation Hospital
2007-2009
American Physician Scientists Association
2009
Hospital for Special Surgery
2009
Cornell University
2009
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a new human with few effective treatments1. Convalescent plasma, donated persons who have recovered from COVID-19, the acellular component of blood that contains antibodies, including those specifically recognize SARS-CoV-2. These when transfused into patients infected SARS-CoV-2, are thought to exert an antiviral effect, suppressing virus replication before mounted their own...
Abstract As breast cancer screening rates increase, smaller and more numerous lesions are being identified earlier, leading to breast-conserving surgical procedures. Achieving a clean margin represents technical challenge with important clinical implications. Optical coherence tomography (OCT) is introduced as an intraoperative high-resolution imaging technique that assesses tumor margins by providing real-time microscopic images up 2 mm beneath the tissue surface. In study of 37 patients...
Magnetic iron oxide nanoparticles and near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWNT) form heterostructured complexes that can be utilized as multimodal bioimaging agents. Fe catalyst-grown SWNT were individually dispersed in aqueous solution via encapsulation by oligonucleotides with the sequence d(GT)15, enriched using a 0.5 T magnetic array. The resulting nanotube show distinct NIR fluorescence, Raman scattering, visible/NIR absorbance features, corresponding to...
Fourier transform light scattering (FTLS) is a novel experimental approach that combines optical microscopy, holography, and for studying inhomogeneous dynamic media. In FTLS the phase amplitude of coherent image field are quantified propagated numerically to plane. Because it detects all scattered angles (spatial frequencies) simultaneously in each point image, can be regarded as spatial equivalent infrared spectroscopy, where temporal frequencies detected at moment time.
Abstract Passive transfer of antibodies from COVID-19 convalescent patients is being used as an experimental treatment for eligible with SARS-CoV-2 infections. The United States Food and Drug Administration’s (FDA) guidelines plasma initially recommended target antibody titers 160. We evaluated neutralizing in sera recovered using plaque reduction neutralization tests (PRNT) at moderate (PRNT50) high (PRNT90) stringency thresholds. found that activity significantly increased time post...
Abstract Dynamic measurements of steroid hormones in vivo are critical, but sensing is currently limited by the availability specific molecular recognition elements due to chemical similarity these hormones. In this work, a new, self‐templating synthetic approach applied using corona phase (CoPhMoRe) targeting family molecules produce near infrared fluorescent, implantable sensors. A key limitation CoPhMoRe has been its reliance on library generation for sensor screening. This problem...
During breast-conserving surgeries, axillary lymph nodes draining from the primary tumor site are removed for disease staging. Although a high number of often resected during sentinel and lymph-node dissections, only relatively small percentage found to be metastatic, fact that must weighed against potential complications such as lymphedema. Without real-time in vivo or situ intraoperative imaging tool provide microscopic assessment nodes, postoperative paraffin section histopathological...
We employ Fourier-transform light scattering, a technique recently developed in our laboratory, to study the scattering properties of rat organ tissues. Using knowledge complex field associated with high-resolution microscope images tissue slices, we extracted mean-free path l(s) and anisotropy factor g, which characterize bulk for three different organs. This "bottom up" approach measuring parameters allows predicting wave transport phenomena within interest at multitude scales-from organelle level.
Physician-scientists, with in-depth training in both medicine and research, are uniquely poised to address pressing challenges at the forefront of biomedicine. In recent years, a number organizations have outlined obstacles maintaining pipeline physician-scientists, classifying them as an endangered species. As in-training early-career physician-scientists across spectrum pipeline, we share here our perspective on current available opportunities that might aid generation becoming independent...
In recent decades, biologists have sought to tag animals with various sensors study aspects of their behavior otherwise inaccessible from controlled laboratory experiments. Despite this, chemical information, both environmental and physiological, remains challenging collect despite its tremendous potential elucidate a wide range animal behaviors. this work, we explore the design, feasibility, data collection constraints implantable, near-infrared fluorescent nanosensors based on DNA-wrapped...
Optical diagnostic imaging techniques are increasingly being used in the clinical environment, allowing for improved screening and diagnosis while minimizing number of invasive procedures. Diffuse optical tomography, example, is capable whole-breast developed as an alternative to traditional X-ray mammography. While this may eventually be a very effective method, other better suited on cellular molecular scale. Coherence Tomography (OCT), instance, high-resolution cross-sectional tissue...
We present a novel needle-based device for the measurement of refractive index and scattering using low-coherence interferometry. Coupled to sample arm an optical coherence tomography system, detects response of, path length through, residing in fixed-width channel. report use make near-infrared measurements tissues materials with known properties. The could be used exploit variations tissue medical biological diagnostics accessible by needle insertion.
Carbon nanotube uptake was measured via high-speed confocal Raman imaging in live cells. Spatial and temporal tracking of two cell-intrinsic nine nanotube-derived bands conducted simultaneously RAW 264.7 macrophages. Movies resolved single (n, m) species, defects, aggregation states nanotubes transiently as well the cell position, denoted by lipid protein signals. This work portends real-time molecular cells tissues using spectroscopy, affording multiplexing complete photostability.
Fluorescent nanosensors hold promise to address analytical challenges in the biopharmaceutical industry. The monitoring of therapeutic protein critical quality attributes such as aggregation is a long-standing challenge requiring low detection limits and multiplexing different product parameters. However, general approaches for interfacing process remain minimally explored date. Herein, we design fabricate integrated fiber optic nanosensor element, measuring sensitivity, response time,...
Abstract Background In 2019, AABB released the bulletin “Recommendations on Use of Group O Red Blood Cells” in which recommendations about pediatric and neonatal blood transfusions were limited. Eight U.S. hospitals sought to determine trends group use clarify populations receive despite a non‐group type. Study Design Methods U.S.‐based institutions serving population provided data from their respective Electronic Health Records. Data submitted included unit type, patient age, sex, discharge...
The COVID-19 pandemic has profoundly affected life worldwide. Governments have been faced with the formidable task of implementing public health measures, such as social distancing, quarantines, and lockdowns, while simultaneously supporting a sluggish economy stimulating research development (R&D) for pandemic. Catalyzing bottom-up entrepreneurship is one method to achieve this. Home-grown efforts by citizens wishing contribute their time resources help sprouted organically, ideas shared...
There is a pressing need for sensors and assays to monitor chemotherapeutic activity within the human body in real time optimize drug dosimetry parameters such as timing, quantity, frequency an effort maximize efficacy while minimizing deleterious cytotoxicity. Herein, we develop near-infrared fluorescent nanosensors based on single walled carbon nanotubes Temozolomide (TMZ) its metabolite 5-aminoimidazole-4-carboxamide using Corona Phase Molecular Recognition synthetic molecular recognition...
Needle biopsy of small or nonpalpable breast lesions has a high nondiagnostic sampling rate even when needle position is guided by stereotaxis ultrasound. We assess the feasibility using near-infrared fiber optic probe and computer-aided detection for microscopic guidance procedures. Specimens from nine consented patients undergoing breast-conserving surgery were assessed intraoperatively device with an integrated fiber-optic capable assessing two physical tissue properties highly correlated...
Reflectance and fluorescence spectroscopies have shown great promise for early detection of epithelial dysplasia. We developed a clinical reflectance spectrofluorimeter multimodal spectroscopic diagnosis This instrument, the FastEEM, collects white light excitation-emission matrices (EEM's) within fraction second. In this paper we describe FastEEM instrumentation, designed collection multi-modal data. illustrate its performance using tissue phantoms with well defined optical properties...
We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis DNA sequencing. To fully the advantages PME, a fluorescent dye set has been developed whose absorption maxima span virtually entire visible spectrum. Unlike emission wavelength-dependent approaches identifying species, removal spectral component PME confers number including higher normalized signals from all dyes...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a highly desmoplastic cancer with limited treatment options. There an urgent need for tools that monitor therapeutic responses in real time. Drugs such as gemcitabine and irinotecan elicit their effect cells by producing hydrogen peroxide (H2O2). In this study, specific DNA-wrapped single-walled carbon nanotubes (SWCNT), which precisely H2O2, were used to determine the response of PDAC vitro tumors vivo. Drug efficacy was evaluated...
Due to its label-free and non-destructive nature, applications of Raman spectroscopic imaging in monitoring therapeutic responses at the cellular level are growing. We have recently developed a high-speed confocal microscopy system image living biological specimens with high spatial resolution sensitivity. In present study, we applied this monitor effects Bortezomib, proteasome inhibitor drug, on multiple myeloma cells. Cluster followed by spectral profiling suggest major differences nuclear...