A5087712232- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Hormonal and reproductive studies
- Reproductive Physiology in Livestock
- Male Reproductive Health Studies
- Yersinia bacterium, plague, ectoparasites research
- Toxoplasma gondii Research Studies
- Animal Diversity and Health Studies
- Reproductive Health and Technologies
- Renal and related cancers
- Educational Innovations and Technology
- Veterinary Equine Medical Research
- Meningioma and schwannoma management
- Education in Rural Contexts
- Ovarian function and disorders
- Comparative Animal Anatomy Studies
- Educational Technology in Learning
- Neurofibromatosis and Schwannoma Cases
- Genetic and phenotypic traits in livestock
- Effects of Environmental Stressors on Livestock
- Seed Germination and Physiology
- Pluripotent Stem Cells Research
University of Buenos Aires
2014-2024
Llama semen is highly viscous. This characteristic usually evaluated subjectively by measuring the thread formed when carefully pippeting a sample of semen. The aims this study were (i) to objectively determine and analyse llama viscosity, (ii) compare viscosity between ejaculates same male as well different males, (iii) correlation other characteristics (iv) evaluate effect collagenase on viscosity. Semen was using cone-plate Brookfield rotational viscometer. A non Newtonian, pseudoplastic...
Sarcocystis sp. are coccidian protozoans belonging to the Apicomplexa phylum. As other members of this phylum, they obligate intracellular parasites having a complex cellular machinery for invasion host cells. spp. display dixenous life cycles, involving predator and prey as definitive intermediate hosts, respectively. Specifically, these develop sarcocysts in tissues their ranging size from microscopic visible naked eye, depending on species. When hosts consume sarcocysts, infective forms...
Sperm head morphology is basically conditioned by the nuclear structure. The aim of present work was to study relation between morphological features, DNA content and chromatin distribution in morphologically normal vs. abnormal bovine spermatozoa. To this end, individual Feulgen-reacted spermatozoa were cytophotometrically studied. Chromatin compactation evaluated means area, as well mean maximal absorbance each nucleus. Morphological abnormality analysed included large, small, pear, narrow...
The aim of this study was to determine the effect two equilibration temperatures (5 °C and room temperature) cryoprotectants (glycerol dimethylformamide, both at 7%) on llama sperm cryopreservation. Llama ejaculates were divided into four aliquots. A lactose-EDTA-egg yolk (LEEY) extender with either 7% glycerol (LEEY-G) or dimethylformamide (LEEY-DMF) added aliquots, which equilibrated for 20 min temperature subsequently frozen. other aliquots extended in LEEY, cooled 5 °C, then LEEY-G...
The aim of this study was to elucidate the role llama seminal plasma in formation oviductal sperm reservoirs. Female llamas with follicles mature phase were mated a bulbourethral glands-removed male. Females nonbulbourethral males used as control. Oviducts obtained by surgery 24 h after mating. uterotubal junction and isthmus examined scanning electron microscopy, mucopolysaccharides identified Alcian blue staining. To know proteins probably involved reservoir formation, SDS-PAGE (8% 18%...
Contents The aim of this study was to carry out in vitro fertilization using spermatozoa selected with Androcoll‐E™ and evaluate the efficiency culture medium DMEM‐F12 for embryo development llama. Twelve adult females from 18 superstimulated (67%) were used as oocyte donors. They 1500 IU eCG after 5 days, received a single dose buserelin. Twenty hours post‐injection, follicular aspiration conducted by flank laparotomy. Semen collections performed under general anesthesia electroejaculation...
Llama production in Argentina has increased, as the international interest breeding this type of animals grown last years. Considering great polymorphism that llama spermatozoa present at evaluation using light microscopy, aim study was to objectively evaluate sperm head morphometry digital morphometric analysis. Five ejaculates from each eight males were obtained parameters 8000 heads stained with Tinción 15(®). The following average results for parameter: size parameters: area 20.09 μm(2),...
In South American camelids, sperm survival is low after thawing and poor results are obtained when artificial insemination performed with cryopreserved semen. The aim of this study was to evaluate the effect different percentages (10% 50%) seminal plasma added prior process cryopreservation also absence on llama freezing thawing. A total 15 ejaculates from five adult males (n = 5; r 3) were evaluated. significant decrease in motility, viability, membrane function intact acrosomes observed...
Seminal plasma (SP) of South American Camelids could interfere with the interaction spermatozoa extenders; therefore it becomes necessary to improve semen management using enzymatic treatment. Our objective was compare two cooling protocols for llama semen. Twelve ejaculates were incubated in 0.1% collagenase and then divided into aliquots. One extended lactose egg yolk (LEY) (Protocol A: SP present). The other aliquot centrifuged, pellet resuspended LEY B: absent). Both samples maintained...
Seminal plasma is intimately connected to sperm physiology and particularly in South American Camelids, has demonstrated be involved multiple physiological reproductive events. Different percentages of seminal (0%, 10% 50%) were added thawed llama semen samples with the objective evaluating interaction cryopreserved over time (0, 1.5 3 hr at 37°C). A total 20 ejaculates from five adult males (n = 5; r 4) evaluated. significant decrease motility, membrane function live was observed all 0 when...
The effect cryopreservation has on sperm chromatin condensation been studied in many species but not South American camelids. objectives of this study were to evaluate with toluidine blue (TB) the effects cooling and adding collagenase llama DNA condensation. optimum incubation time (30 s, 1.5 3 min) a reducing agent (dithiothreitol) was also determined. When comparing cooled samples raw ejaculate, significant increase showing high degree decondensation (TB positive) observed (P = 0.005). A...