A5021147208- RNA modifications and cancer
- Metabolism and Genetic Disorders
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Folate and B Vitamins Research
- Biochemical and Molecular Research
- Ferroptosis and cancer prognosis
- Bioinformatics and Genomic Networks
- Electrochemical sensors and biosensors
- Gene Regulatory Network Analysis
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Genomics and Rare Diseases
- Histone Deacetylase Inhibitors Research
- Reproductive Biology and Fertility
- Peptidase Inhibition and Analysis
- Electrochemical Analysis and Applications
- Renal and related cancers
- Cancer-related gene regulation
- Neonatal Health and Biochemistry
- Lung Cancer Treatments and Mutations
- Phytochemical compounds biological activities
Second Xiangya Hospital of Central South University
2018-2025
Central South University
2010-2025
Shanghai Changzheng Hospital
2023-2024
Hangzhou Children's Hospital
2021
Yale University
2015-2017
Fuzhou Maternity and Child Health Care Hospital
2017
Jiujiang First People's Hospital
2015
SAIC Motor (China)
2011
Emory University
2010
Xiangya Hospital Central South University
2010
Background:The JAK/STAT signaling pathway is the main inflammatory signal transduction pathway, whether contributes pathology of SCI and targeting will alleviate needs to be addressed.Here, we explored therapeutic effect pan-JAK inhibitor tofacitinib (TOF) on secondary injury after explained underlying mechanisms.Methods: model in rat was established evaluate effects TOF treatment vivo.Histological behavioral analyses were performed at different time points SCI.In vitro, pro-inflammatory...
Klotho gene, a new anti-aging is mainly expressed in the kidney tubules. Several studies have found relationship between klotho and emergence development of renal diseases. This study set out to explore role fosinopril (Fos) valsartan (Val) on expression induced by angiotensin II (Ang II) rat tubular epithelial cells (NRK-52E).NRK-52E were divided into five groups according treatment Ang II, Fos Val. Transforming growth factor-β1 (TGF-β1), p38, phospho-p38 (p-p38), p53, Sp1 protein...
Despite the examples of protein evolution via mutations in coding sequences, we have very limited understanding on gene network changes cis-regulatory elements. Using galactose as a model, here show how regulatory promoters contribute to evolved activity between two yeast species. In Saccharomyces cerevisiae, combinatorially replace all by their counterparts from paradoxus, measure resulting inducibility profiles, and model results. Lowering relative strength GAL80-mediated negative feedback...
According to mounting evidence, long noncoding RNAs (lncRNAs) play a vital role in regulated cell death (RCD). A potential strategy for cancer therapy involves triggering ferroptosis, novel form of RCD. Although it is thought be an autophagy-dependent process, still unclear how the two processes interact. This study characterized intergenic RNA, LINC00551, expressed at low level lung adenocarcinoma (LUAD) and some other cancers. Overexpression LINC00551 suppresses viability while promoting...
Abstract Noise-induced heterogeneity in gene expression is an inherent reality for cells. However, it not well understood how noise strength changes a single while the host cell aging. Using state-of-the-art microfluidic platform, we measure dynamics aging yeast cells by tracking generation-specific activity of canonical GAL1 promoter. We observe reduction during normal cell, followed short catastrophe phase which increased. hypothesize that aging-associated increases chromatin state...
Certain studies have indicated that naringin possesses various pharmacological activities including anti-aging, anti-oxidation, anticancer, cardiovascular and cerebrovascular disease prevention, in addition to anti-hepatic effects. The present study explores the anticancer effect of on human small cell lung cancer H69AR cells. Cell growth apoptosis rates cells were measured by MTT or flow cytometry, which demonstrated suppressed induced MicroRNA (miR)-126 expression levels phosphorylated...
Aberrant N6-methyladenosine (m6A) RNA methylation regulatory genes and related gene alternative splicing (AS) could be used to predict the prognosis of non–small cell lung carcinoma. This study focused on 13 m6A (METTL3, METTL14, WTAP, KIAA1429, RBM15, ZC3H13, YTHDC1, YTHDC2, YTHDF1, YTHDF2, HNRNPC, FTO, ALKBH5) expression profiles in TCGA-LUAD ( n = 504) TCGA-LUSC 479) datasets from Cancer Genome Atlas database. The data were downloaded bioinformatically statistically analyzed, including...
Abstract Accumulation of vimentin is the core event in epithelial–mesenchymal transition (EMT). Post‐translational modifications have been widely reported to play crucial roles imparting different properties and functions vimentin. Here, a novel modification vimentin, acetylated at Lys 104 (vimentin‐K104Ac) identified, which stable lung adenocarcinoma (LUAD) cells. Mechanistically, NACHT, LRR, PYD domain‐containing protein 11 (NLRP11), regulator inflammatory response, bind promote...
Abstract Epigenetic regulation of metabolism profoundly influences cell fate commitment. During osteoclast differentiation, the activation RANK signaling is accompanied by metabolic reprogramming, but epigenetic mechanisms which induces this reprogramming remain elusive. By transcriptional sequence and ATAC analysis, study identifies that upregulates PRMT6 modification, triggering a switching from fatty acids oxidation toward glycolysis. Conversely, Prmt6 deficiency reverses shift, markedly...
Vitrification of embryos is a routine procedure in IVF (in vitro fertilization) laboratories. In the present study, we aimed to investigate effect vitrification on mouse preimplantation embryo development vitro, and epigenetic status imprinted gene Grb10 embryos. The blastocyst formation rate for vitrified 8-cell was similar non-vitrified embryos, whereas hatching lower than that group. expression level major-type transcript decreased significantly blastocysts compared with vivo blastocysts....
Drug resistance after long-term use of Tyrosine kinase inhibitors (TKIs) has become an obstacle for prolonging the survival time patients with clear cell renal carcinoma (ccRCC). Here, genome-wide CRISPR-based screening to reveal that HDAC8 is involved in decreasing sensitivity ccRCC cells sunitinib applied. Mechanically, deacetylated ETS1 at K245 site promote interaction between and HIF-2α enhance transcriptional activity ETS1/HIF-2α complex. However, antitumor effect inhibiting on...
Abstract Genetic noise together with genome duplication and volume changes during cell cycle are significant contributors to cell-to-cell heterogeneity. How can cells buffer the effects of these unavoidable epigenetic genetic variations on phenotypes that sensitive such variations? Here we show a simple network motif is essential for network-dosage compensation reduce extrinsic output. Using natural synthetic gene networks without motif, measure activity in single yeast find compensated...
Newborn screening for primary carnitine deficiency (NBS) is commonly implemented worldwide; however, it has poor sensitivity. This study aimed to evaluate the feasibility of improving by including a second-tier genetic assay.An Agena iPLEX assay was developed identify 17 common SLC22A5 mutations in Chinese populations and applied NBS as screening. From January 2017 December 2018, 204,777 newborns were screened PCD using tandem mass spectrometry. A total 316 (0.15%) residual NBS-positive...
An imbalance in ROS (reactive oxidative species) and the antioxidant barrier regulates process of tumorigenesis. GSH has a key effect preventing cells from damage by scavenging ROS. The role CHAC2, an enzyme regulating GSH, lung adenocarcinoma remains unknown. Here, RNA sequencing data analysis immunohistochemistry (IHC) assays normal tissues were used to verify expression CHAC2. CHAC2 on proliferation abilities was examined using series overexpression or knockout assays. IHC results showed...
Abstract Background Glutaric acidemia type 1 (GA1) is a treatable disorder affecting cerebral organic acid metabolism caused by defective glutaryl-CoA dehydrogenase ( GCDH ) gene. GA1 diagnosis reports following newborn screening (NBS) are scarce in the Chinese population. This study aimed to assess acylcarnitine profiles and genetic characteristics of patients with identified through NBS. Results From January 2014 September 2020, 517,484 newborns were screened tandem mass spectrometry, 102...
Despite the development of various modeling approaches to predict gene network activity, a time dynamic stochastic model taking into account real-time changes in cell volume and cycle stages is still missing. Here we present single-cell that can be applied any eukaryotic with number components. The tracks volume, DNA replication, division, dynamically adjusts rates reactions based on this information. By tracking maintain lineage information, allowing researcher trace descendants single...
Abstract Objectives Multiple acyl-CoA dehydrogenase deficiency (MADD) is an autosomal recessive disorder of fatty acid, amino acid and choline metabolism. Late-onset MADD caused by ETFDH mutations the most common lipid storage myopathy in China. However, few patients with have been identified through newborn screening (NBS). This study assessed acylcarnitine profiles molecular features NBS. Methods From January 2014 to June 2020, 479,786 newborns screened via tandem mass spectrometry were...