Although per- and polyfluoroalkyl substances (PFASs) have always been a key issue in the global environmental field, there are still lot of undiscovered PFASs environment due to new PFAS alternatives developed by manufacturers. Wastewater treatment plants (WWTPs), as one sources for PFASs, an important part process releasing into environment. In this study, suspect screening homologue analysis with quadrupole time-of-flight tandem mass spectrometry were used discover wastewater from WWTP...

Airborne particulate matter (APM) has an important role in inhalation exposure, especially China. The environmental occurrence of conventional and unknown per- polyfluoroalkyl substances (PFASs) APM remains unclear. Therefore, this study, a two-stage experiment was designed to identify potential PFASs investigate their distribution APM. Indoor outdoor samples were collected from five selected cities Through PFAS homologue analysis suspect screening, 50 peaks identified with different...

Abstract Carbon dots (CDs) are photoluminescent nanomaterials with wide-ranging applications. Despite their photoactivity, it remains unknown whether CDs degrade under illumination and such photodegradation poses any cytotoxic effects. Here, we show laboratory-synthesized irradiated light into molecules that toxic to both normal (HEK-293) cancerous (HeLa HepG2) human cells. Eight days of irradiation photolyzes 28.6-59.8% the <3 kilo Dalton molecules, 1431 which detected by...

Novel per- and polyfluoroalkyl substances (PFASs) in various environmental media have attracted increasing attention; however, the information regarding PFASs exposure pregnant women fetuses is insufficient. In this study, we built applied suspect nontarget screening strategies based on mass difference of CF2, CF2O, CH2CF2 units to select potential novel from 117 paired maternal cord sera. total, 10 legacy 19 classes were identified be above confidence levels 3, among which 14 not previously...

Perfluorooctanoic acid (PFOA), a perfluoroalkyl acid, can result in hepatotoxicity and neurobehavioral effects animals. The metabolome, which serves as connection among transcriptome, proteome toxic effects, provides pathway-based insights into of PFOA. Since understanding changes the metabolic profile during neurotoxicity were still incomplete, high-throughput targeted metabolomics approach (278 metabolites) was used to investigate exposure PFOA for 28 d on brain liver male Balb/c mice....

Pesticides and pharmaceuticals are widely used in modern life discharged into wastewater after usage. However, a large number of transformation products (TPs) formed through abiotic (hydrolysis/photolysis, etc.) biotic (aerobic/anaerobic degradation by micro-organisms) treatment processes, the structure potential risk TPs still unclear. In this study, suspect non-target screening was performed to monitor these chemicals with HPLC-QTOF-MS. We identified 60 parent compounds three Chinese...

Novel per- and polyfluoroalkyl substances (PFASs) have become a key issue in global environmental studies. Although several novel PFASs been discovered atmospheric particulate matter through nontarget analysis, information on the occurrence of gaseous phases conventional sampling techniques is somewhat deficient. Therefore, this Article describes new type air sampler, cryogenic sampler (CAS), which was used to collect all components simultaneously. Nontarget analysis then performed homologue...

Unknown forever chemicals like per- and polyfluoroalkyl substances (PFASs) are difficult to identify. Current platforms designed for metabolites natural products cannot capture the diverse structural characteristics of PFAS. Here, we report an automatic PFAS identification platform (APP-ID) that screens in environmental samples using enhanced molecular network identifies unknown structures machine learning. Our networking algorithm, which enhances characteristic fragment matches, has lower...

Abstract The rapid increase of novel per- and polyfluoroalkyl substances (PFAS) raises concerns, while their identification remains challenging. Here, we develop a two-layer homolog network approach for PFAS nontarget screening using mass spectrometry. first layer constructs networks between homologs, with evaluation showing that it filters 94% false candidates. second builds classes to expedite the PFAS. We detected 94 in twelve waterproof products two related industrial sludges, including...

The spatial distribution of 10 perfluoroalkyl acids including linear and branched (six monotrifluoromethyl isomers) perfluorooctane sulfonate (PFOS) in surface water was investigated Huai River Basin Taihu Lake Jiangsu Province, China. In the samples from Basin, perfluorooctanoic acid (PFOA) PFOS were predominant compounds (mean 18 ng/L 4.7 ng/L, respectively), while Lake, PFOA, perfluorohexanoic (PFHxA), 56 19 15 respectively). Branched (Br-PFOS) isomers accounting for 48.1% to 62.5% total...

The effects of 2-ethylhexyl diphenyl phosphate (EHDPP) on cytotoxicity and mRNA expression, as well its metabolism, were investigated using a chicken embryonic hepatocyte (CEH) assay. After incubation for 36 h, the lethal concentration 50 (LC50) was ± 11 μM, suggesting that EHDPP is one small cohort highly toxic organophosphate esters (OPEs). By use ToxChip polymerase chain reaction (PCR) array, we report modulation 6, 11, or 16/43 genes in CEH following exposure to 0.1, 1, 10 μM EHDPP,...

Since organophosphate (OP) triesters are ubiquitous in environmental matrices, there is an increasing concern regarding human exposure to OP or their metabolites. In this study, we measured levels of 16 and 4 diesters n = 99 blood samples non-occupationally exposed adults (aged 18-87) from Jiangsu Province, eastern China. Based on the concentrations, statistical difference correlativity were calculated characterize population diversity potential sources triester diester. Di (2-ethylhexyl)...

Perfluoroalkyl substances (PFASs) exist extensively and several of these have been verified to be toxic. Prenatal exposure PFASs has attracted much attention. Metabolome-wide association analyses can used explore the toxicity mechanisms by identifying associated biomarkers.To evaluate associations between metabolites in maternal cord serum internal common PFASs.Paired samples were collected from 84 pregnant women who gave birth 2015 2016. Seven legacy two novel measured. A nontarget...

Food is a major source of human exposure to perfluorooctanoic acid (PFOA), however, PFOA bioavailability in food has not been studied. An vivo mouse model and three vitro methods (unified BARGE method, UBM; physiologically based extraction test, PBET; digestion IVD) were used determine the relative bioaccessibility presence 17 foods. was mixed with foods different nutritional compositions fed mice over 7-d period. determined by comparing accumulation liver following via that water. water...

Novel per- and polyfluoroalkyl substances (PFASs) in the environment populations have received extensive attention; however, their distribution potential toxic effects general population remain unclear. Here, a comprehensive study on PFAS screening was carried out serum samples of 202 individuals from four cities China. A total 165 suspected PFASs were identified using target nontarget analysis, including seven homolog series, which 16 validated against standards, [4:2 chlorinated...

Antimicrobial transformation products (ATPs) in the environment have raised extensive concerns recent years due to their potential health risks. However, only a few ATPs been investigated, and most of pathways antimicrobials not completely elucidated. In this study, we developed nontarget screening strategy based on molecular networks detect identify pharmaceutical wastewater. We identified 52 49 (TPs) with confidence level three or above. Thirty TPs had previously reported environment....

Silver nanoparticles (AgNPs) are one of the most widely used NPs. Their adverse effects on either host or its gut microbiota (GM) have been examined. Nevertheless, whether GM plays any role in AgNP toxicity to remains unclear. In present study, AgNPs were administered mice by oral gavage once a day for 120 days. A significant dose-dependent accumulation Ag liver was observed, with steady state reached within 21 The changed structure GM, mainly respect microorganisms involved metabolism...