A5102013873- Antibiotic Resistance in Bacteria
- Fish Biology and Ecology Studies
- Fish Ecology and Management Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Advanced oxidation water treatment
- RNA modifications and cancer
- Bacterial biofilms and quorum sensing
- Identification and Quantification in Food
- Microbial bioremediation and biosurfactants
- Bacteriophages and microbial interactions
- Asymmetric Synthesis and Catalysis
- Toxic Organic Pollutants Impact
- Infections and bacterial resistance
- Chemical Synthesis and Analysis
- Bacterial Genetics and Biotechnology
- Aquatic Invertebrate Ecology and Behavior
- Environmental Quality and Pollution
- Fish biology, ecology, and behavior
- Toxin Mechanisms and Immunotoxins
- Aquatic Ecosystems and Biodiversity
- Enterobacteriaceae and Cronobacter Research
- Sirtuins and Resveratrol in Medicine
- Ichthyology and Marine Biology
Guangdong University of Technology
2025
China West Normal University
2011-2024
University of South China
2024
Shenzhen University
2022-2023
Second Affiliated Hospital of Zhejiang University
2019-2023
Texas A&M University
2013-2023
Texas A&M Health Science Center
2021-2023
Zhengzhou University
2022
Nanjing University
2022
Chinese Academy of Fishery Sciences
2021
Nε-Acryloyl-l-lysine, a noncanonical amino acid with an electron deficient olefin, is genetically encoded in Escherichia coli using pyrrolysyl-tRNA synthetase mutant coordination tRNACUAPyl. The acrylamide moiety stable cells, whereas it active enough to perform diverse set of unique reactions for protein modifications vitro. These include 1,4-addition, radical polymerization, and 1,3-dipolar cycloaddition. We demonstrate that incorporated Nε-acryloyl-l-lysine efficiently modified...
Abstract Emergence of tigecycline-resistance tet (X) gene orthologues rendered tigecycline ineffective as last-resort antibiotic. To understand the potential origin and transmission mechanisms these genes, we survey prevalence its in 2997 clinical E. coli K. pneumoniae isolates collected nationwide China with results showing very low on two types strains, 0.32% 0%, respectively. Further surveillance 3692 different Gram-negative bacterial strains during 1994–2019 hospitals Zhejiang province,...
TMexCD1-TOprJ1, which is associated with phenotypic resistance to multiple classes of antibiotics, a transmissible resistance-nodulation-division (RND) family efflux pump. However, the prevalence and genomic characteristics clinical isolates this important determinant are poorly understood. In study, we aimed survey tmexCD-toprJ among Gram-negative collected from hospitals in China between 1991 2020 characterise tmexCD-toprJ-positive isolates.We conducted online data retrieval active...
Acinetobacter baumannii is one of the key Gram-negative pathogens that can cause serious nosocomial infections. In China, a large proportion clinical A. strains are multidrug resistant, among which resistant to carbapenems particularly worrisome, as infections caused by such may limit choice existing antibiotics. We conducted nationwide and genome-based surveillance on prevalence antibiotic susceptibility profile carbapenem-resistant (CRAB) collected from intensive care units (ICUs) in...
The genetically encoded photo-cross-linkers promise to offer a temporally controlled tool map transient and dynamic protein-protein interaction complexes in living cells. Here we report the synthesis of panel 2-aryl-5-carboxytetrazole-lysine analogs (ACTKs) their site-specific incorporation into proteins via amber codon suppression Escherichia coli mammalian Among five ACTKs investigated, N-methylpyrroletetrazole-lysine (mPyTK) was found give robust site-selective photo-cross-linking...
Abstract Using amber suppression in coordination with a mutant pyrrolysyl‐tRNA synthetase‐tRNA Pyl pair, azidonorleucine is genetically encoded E. coli . Its genetic incorporation followed by traceless Staudinger ligation phosphinothioester allows the convenient synthesis of protein site‐specifically installed lysine acylation. By simply changing identity, any acylation type could be introduced. this approach, we demonstrated that both acetylation and succinylation can selectively ubiquitin...
Summary Klebsiella pneumoniae has emerged as one of the most important pathogens that frequently encounter in community‐acquired or hospital‐acquired infections. Timely epidemiological surveillance could greatly facilitate infection control K. and many deadly alike. In this study, we evaluated performance IR Biotyper, a Fourier transform infrared (FTIR) spectroscopy system for isolates typing through (i) optimizing culture scheme defining cutoff value (COV) range (ii) comparing with commonly...
Health care-associated infections, also known as nosocomial are the most frequent adverse events in health care delivery worldwide, causing high rates of morbidity and mortality costs. Pseudomonas aeruginosa is one leading bacterial species infections. Carbapenems effective antimicrobial agents for treatment its severe
The highly efficient, regioselective, and enantioselective transfer hydrogenation of α-keto ketimines reductive amination diketones by Brønsted acid catalysis is described. A series chiral α-amino ketones prepared in high yields (up to >99%), excellent regioselectivities >99:1), enantioselectivities 98% ee). This method has broad substrate scope.
Benzo[a]pyrene of natural and anthropogenic sources is one the toxic, mutagenic, polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants. This study focuses on an integrated treatment benzo[a]pyrene involving sequential chemical oxidation biological degradation. The objectives are to (1) provide mechanistic details in ozone-mediated degradation aqueous phase, (2) test biodegradability resultant intermediates, (3) feasibility for coupled chemical-biological five-ring PAH. Batch...
Abstract Using the amber suppression approach, N ϵ ‐(4‐azidobenzoxycarbonyl)‐δ,ϵ‐dehydrolysine, an allysine precursor is genetically encoded in E. coli. Its genetic incorporation followed by two sequential biocompatible reactions allows convenient synthesis of proteins with site‐specific lysine dimethylation. this dimethyl‐histone H3 and p53 have been synthesized used to probe functions epigenetic enzymes including histone demethylase LSD1 acetyltransferase Tip60. We confirmed that...
The rare AGG codon in Escherichia coli has been reassigned to code non-canonical amino acids (ncAAs) by using the PylRS-tRNA(Pyl)(CCU) pair. When N(ε) -alloc-lysine was used as a PylRS substrate, almost quantitative occupancy of at an site achieved minimal medium. ncAAs can be potentially incorporated with varying efficiencies, depending on their activities towards corresponding enzymes. As is sense codon, approach reported here resolves typical low ncAA incorporation issue that associated...
Three carbapenem-resistant Klebsiella pneumoniae (CRKP; strains KP-426, KP-C76, and KP-CT77) were isolated from a patient with severe burns during the treatment of colistin tigecycline. Single-nucleotide polymorphism typing showed that three ST11 CRKP clonally related. isolates harbored same set antimicrobial resistance genes. bla KPC-2 , SHV-12 TEM-1 rmtB genes located on 128,928-bp IncFII/IncR plasmid. Tet (A), catA2 sul2 dfrA14 plasmid an unknown Inc-type. SHV-11 fosA aadA2 chromosomal An...
Incorporating functional metrics into the development of a diatom-based index biotic integrity (D-IBI) is conducive to more comprehensive assessment water quality and degree external impact on ecosystem function. A D-IBI was developed by incorporating candidate metrics. Four were selected divided five levels evaluate 147 sampling sites. By comparing scores in Thousand Islands Lake (TIL) catchment, we found that total score ranged from 20 100 (median 45). Compared with reference sites,...
Schizothorax kozlovi is an endemic and vulnerable fish species found in the upper Yangtze River China. Over past few years, population resources of S. have been nearly completely depleted owing to multiple contributing threats. While complete mitochondrial genomes serve as important molecular markers for phylogenetic genetic studies, genome has still received little attention. In this study, we analyzed characterization investigated relationships Schizothorax. The was 16,585 bp length, which...
By evolving the N-terminal domain of Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS) that directly interacts with tRNAPyl , a mutant clone displaying improved amber-suppression efficiency for genetic incorporation Nϵ -(tert-butoxycarbonyl)-l-lysine threefold more than wild type was identified. The identified mutations were R19H/H29R/T122S. Direct transfer these to two other PylRS mutants previously evolved -acetyl-l-lysine and -(4-azidobenzoxycarbonyl)-l-δ,ϵ-dehydrolysine also...
Abstract Voyager 2 crossed the heliopause in 2018 and observed a small-amplitude modulation of galactic cosmic rays beyond heliopause, within boundary layer approximately 0.57 au thick. Prior to this, it detected similar for both magnetic field, with scale about 0.82 inside heliopause. In this study, we investigate near using numerical simulations that account layers on sides Our results indicate leads significant reduction parallel diffusion coefficient rays, compared values further into...