A5013637327- Evolution and Genetic Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Chromosomal and Genetic Variations
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Genetic factors in colorectal cancer
- DNA Repair Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- biodegradable polymer synthesis and properties
- Inflammatory Bowel Disease
- Coral and Marine Ecosystems Studies
- Steroid Chemistry and Biochemistry
- Electrospun Nanofibers in Biomedical Applications
- Cancer Mechanisms and Therapy
- RNA modifications and cancer
- Gut microbiota and health
- Reproductive System and Pregnancy
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Marine Ecology and Invasive Species
- Advanced biosensing and bioanalysis techniques
- Hydrogels: synthesis, properties, applications
Instituto Gulbenkian de Ciência
2020-2022
Texas A&M University
2017-2021
University of Lisbon
2021
Wyoming Department of Education
2014
University of Wyoming
2010-2012
Amgen (Germany)
2010
Leibniz Association
2007
LMU Klinikum
2004
Ludwig-Maximilians-Universität München
2004
University Hospital of Bern
2004
Genomes of animals as different sponges and humans show conservation global architecture. Here we that multiple genomic features including transposon diversity, developmental gene repertoire, physical order, intron-exon organization are shattered in the tunicate Oikopleura, belonging to sister group vertebrates retaining chordate morphology. Ancestral architecture animal genomes can be deeply modified may therefore largely nonadaptive. This rapidly evolving lineage thus offers unique...
Abstract How and at what pace bacteria evolve when colonizing healthy hosts remains unclear. Here, by monitoring evolution for more than six thousand generations in the mouse gut, we show that successful colonization of an invader Escherichia coli depends on diversity existing microbiota presence a closely related strain. Following colonization, two modes were observed: one which diversifying selection leads to long-term coexistence ecotypes second directional propels selective sweeps. These...
Significance Copy-number variants are ubiquitous in nature, yet their immediate functional consequences obscure. We conducted a spontaneous mutation accumulation experiment at varying sizes Caenorhabditis elegans , thereby enabling the simultaneous investigation of mutational input and strength selection on evolution copy-number changes. Whole-genome sequencing reveals highest genome-wide rate gene duplication for any species thus far. Our transcriptome analysis further demonstrates that...
Gene duplication is an important process in the functional divergence of genes and genomes. Several processes have been described that lead to duplicate gene retention over different timescales after both smaller-scale events whole-genome duplication, including neofunctionalization, subfunctionalization, dosage balance. Two common modes loss include nonfunctionalization due population dynamics (failed fixation). Previous work has characterized expectations under neofunctionalization...
Mitochondrial genomes of metazoans, given their elevated rates evolution, have served as pivotal markers for phylogeographic studies and recent phylogenetic events. In order to determine the dynamics spontaneous mitochondrial mutations in small populations absence presence selection, we evolved mutation accumulation (MA) lines Caenorhabditis elegans parallel over 409 consecutive generations at three varying population sizes N = 1, 10, 100 hermaphrodites. The =1 should a minimal influence...
Abstract The objective of this study was to develop novel absorbable films suitable for use as a tissue‐engineering scaffold keratinocytes therapy replacement damaged skin. Poly(4‐hydroxybutyrate) (P(4HB)) and poly (3‐hydroxybutyrate) (P(3HB)) were blended with small amounts the polysaccharides hyaluronic acid (HA), chitosan (CH), pectin alginic acid, solution cast produce porous films. resulting composites had favorable mechanical properties, these compared two commercially available...
Abstract Experimental investigations into the rates and fitness effects of spontaneous mutations are fundamental to our understanding evolutionary process. To gain insights molecular consequences mutations, we conducted a mutation accumulation (MA) experiment at varying population sizes in nematode Caenorhabditis elegans, evolving 35 lines parallel for 409 generations three (N = 1, 10, 100 individuals). Here, focus on nuclear SNPs small insertion/deletions (indels) under minimal influence...
Abstract DNA mismatch repair (MMR), an evolutionarily conserved pathway shared by prokaryotic and eukaryotic species alike, influences molecular evolution detecting correcting mismatches, thereby protecting genetic fidelity, reducing the mutational load, preventing lethality. Herein we conduct first genome-wide evaluation of alterations to mutation rate spectrum under impaired activity MutSα homolog, msh-2, in Caenorhabditis elegans male–female fog-2(lf) lines. We performed accumulation (MA)...
ABSTRACT The ecology and environment of the microbes that inhabit mammalian intestine undergoes several changes as host ages. Here, we ask if selection pressure experienced by a new strain colonizing aging gut differs from in young adults. Using experimental evolution mice after short antibiotic treatment, model for common clinical situation, show E. coli rapidly adapts to via both mutation accumulation bacteriophage-mediated horizontal gene transfer (HGT). pattern is characterized larger...
Abstract Background Transposable elements (TEs) are an almost universal constituent of eukaryotic genomes. In animals, Piwi-interacting small RNAs (piRNAs) and repressive chromatin often play crucial roles in preventing TE transcription thus restricting activity. Nevertheless, content varies widely across eukaryotes the dynamics activity silencing evolutionary time is poorly understood. Results Here, we used experimentally evolved populations C. elegans to study expression over 409...
Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' bacteria, especially aquatic ones, is less clear. We studied two Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, one non-TK1 dNK, efficiently deoxyadenosine slightly also deoxycytosine. Surprisingly, four tested dNKs not...
Abstract How and at what pace bacteria evolve when colonizing healthy hosts remains unclear. Here, by monitoring evolution for more than six thousand generations in the mouse gut, we show that successful colonization of an invader Escherichia coli depends on diversity existing microbiota presence a closely related strain. Following colonization, two modes were observed: one which diversifying selection leads to long-term coexistence ecotypes second directional propels selective sweeps. These...
Abstract Transposable elements (TEs) are an almost universal constituent of eukaryotic genomes. In animals, Piwi-interacting small RNAs (piRNAs) and repressive chromatin often play crucial roles in preventing TE transcription thus restricting activity. Nevertheless, content varies widely across eukaryotes the dynamics activity silencing evolutionary time is poorly understood. Here we used experimentally evolved populations C. elegans to study expression over 400 generations. The experimental...
ABSTRACT To ascertain the effect of relaxation selection on global gene expression, Caenorhabditis elegans mutation accumulation (MA) lines were propagated under varying degrees efficiency determined by their different population sizes ( N = 1, 10 and 100). Both mutational variance V m ), residual r ) greatest in MA with lowest natural selection. The results suggest that expression is strong balancing Furthermore, mutations resulting increased transcriptional noise or sensitivity to...
Hintergrund: Eine Subgruppe von Morbus Crohn Patienten (CD) entwickelt Antikörper gegen Mannan, einen Zellwandbestandteil der Hefe Saccharomyces cerevisiae (ASCA) jedoch ist wenig bekannt über die zelluläre Immunantwort oder Mechanismen, welche Antikörperproduktion triggern. Mannan-binding-lectin (MBL) Bestandteil des unspezifischen Immunsystems, welches in Lage ist, an bakterielle Hefe-Partikel zu binden und mit eliminieren. Genetische Polymorphismen verursachen niedrige MBL Serum-Konzentrationen.
ABSTRACT Experimental investigations into the rates and fitness effects of spontaneous mutations are fundamental for our understanding evolutionary process. To gain insights molecular consequences mutations, we conducted a mutation accumulation (MA) experiment at varying population sizes in nematode Caenorhabditis elegans , evolving 35 lines parallel 409 generations three ( N = 1, 10, 100 individuals). Here, focus on nuclear SNPs small indels under minimal influence selection, as well their...
ABSTRACT DNA mismatch repair (MMR), an evolutionarily conserved pathway shared by prokaryotic and eukaryotic species alike, influences molecular evolution detecting correcting mismatches that escape polymerase proofreading, thereby protecting genetic fidelity, reducing the mutational load, preventing lethality. Herein we conduct first genome-wide evaluation of alterations to mutation rate spectrum under impaired activity MutSα homolog, msh-2 , in Caenorhabditis elegans . We performed...