Soil microbiota can confer fitness advantages to plants and increase crop resilience drought other abiotic stressors. However, there is little evidence on the mechanisms correlating a microbial trait with plant stress tolerance. Here, we report that Streptomyces effectively alleviate salinity by producing spiroketal polyketide pteridic acid H (1) its isomer F (2), both of which promote root growth in Arabidopsis at concentration 1.3 nM under stress. Transcriptomics profiles show increased...

In-built diversification: Four ansa macrolides, divergolides A–D (1–4), with intriguing structures were isolated from a bacterial endophyte of the mangrove tree Bruguiera gymnorrhiza. These compounds are generated by common biogenesis linear precursor that undergoes various reactions to give multicyclic structures. This remarkable plasticity results in metabolites different antibacterial and cytotoxic properties. Detailed facts importance specialist readers published as ”Supporting...

Five novel eudesmene-type sesquiterpenes, kandenols A–E (1–5), have been isolated from Streptomyces sp. HKI0595 derived the mangrove plant Kandelia candel. Their structures were established through NMR and mass spectrometry, absolute configurations by Mosher method comparison of CD spectra with α-rotunol β-rotunol. The are reminiscent various plant-derived eudesmenes, yet B C unusual because their hydroperoxide moieties. Kandenol E is first bacterial agarofuran, which belongs to an important...

Investigating microbial interactions from an ecological perspective is a particularly fruitful approach to unveil both new chemistry and bioactivity. Microbial predator-prey in particular rely on natural products as signal or defense molecules. In this context, we identified grazing-resistant Pseudomonas strain, isolated the bacterivorous amoeba Dictyostelium discoideum. Genome analysis of bacterium revealed presence two biosynthetic gene clusters that were found adjacent each other...

Abstract The objective of this study was to develop novel absorbable films suitable for use as a tissue‐engineering scaffold keratinocytes therapy replacement damaged skin. Poly(4‐hydroxybutyrate) (P(4HB)) and poly (3‐hydroxybutyrate) (P(3HB)) were blended with small amounts the polysaccharides hyaluronic acid (HA), chitosan (CH), pectin alginic acid, solution cast produce porous films. resulting composites had favorable mechanical properties, these compared two commercially available...

Abstract Psilocybin, the principal indole alkaloid of Psilocybe mushrooms, is currently undergoing clinical trials as a medication against treatment‐resistant depression and major depressive disorder. The psilocybin supply for pharmaceutical purposes met by synthetic chemistry. We replaced problematic phosphorylation step during synthesis with mushroom kinase PsiK. This enzyme was biochemically characterized used to produce one gram from psilocin within 20 minutes. also describe pilot‐scale...

Abstract To assess the potential use of hyaluronic acid (HA) as adjuvant therapy in rheumatoid arthritis, anti-inflammatory and chondroprotective effects HA were analysed experimental rat antigen-induced arthritis (AIA). Lewis rats with AIA subjected to short-term (days 1 8, n = 10) or long-term 1, 15 22, intra-articular treatment microbially manufactured, high-molecular-weight (molecular weight, 1.7 × 10 6 Da; 0.5 mg/dose). In both tests, buffer-treated served arthritic controls six healthy...

ABSTRACT The functional investigation of redox cofactors is important for many potential biocatalytic processes, yet limited access to these molecules often hampering efficient research activities. Deazaflavin‐dependent enzymes mediate a range biochemical reactions in prokaryotes. Coenzyme F 420 ‐dependent are interesting asymmetric biocatalysis and other challenging reactions, but low titers harvested from natural producers engineered host strains so far limit intensive enzymes. O precursor...

The effect of triterpenes and flavonoids on the activity several hyaluronic acid-splitting enzymes was investigated. Studies showed that inhibitory glycyrrhizin glycyrrhetinic acid is dependent source hyaluronate lyase. Hyaluronate lyase from Streptococcus agalactiae (Hyal B) recombinant (rHyal demonstrated strongest inhibition. In contrast, lyases Streptomyces hyalurolyticus S), equisimilis C) hyaluronidase bovine testis (Dase) only reduced inhibition action. A non-competitive dead end with...

Gilvocarcin-type polyketide glycosides represent some of the most powerful antitumor therapeutics. Bioactivity-guided fractionation a culture extract Streptomyces polyformus sp. nov. (YIM 33176) yielded known gilvocarcin V (2) and novel related compound, polycarcin (1). Structure elucidation by NMR chemical derivatization revealed that congener (1) features C-glycosidically linked alpha-L-rhamnopyranosyl moiety in lieu D-fucofuranose. The concomitant production two distinct furanosyl...

Two new pyrrole alkaloids, N-[4-(2-formyl-5-hydroxymethyl-pyrrol-1-yl)-butyl]-acetamide (1) and N-[5-(2-formyl-5-hydroxymethyl-pyrrol-1-yl)-pentyl]-acetamide (2), a indole derivative (3aR,8aR)-3a-acetoxyl-1,2,3,3a,8,8a-hexahydropyrrolo-[2,3-b]indol (3) were isolated, together with ( − )-3a-hydroxyfuroindoline, (3aR,8aS)-1-acetyl-1,3,3a,8,8a-hexahydropyrrolo-[2,3-b]indol-3a-ol, N-acetyltryptamine A, from an endophytic ascomycetous fungus, Fusarium incarnatum (HKI00504), which was isolated the...

Eingebaute Diversität: Aus dem bakteriellen Endophyten des Mangroven-Baums Bruguiera gymnorrhiza wurden vier Ansamakrolide mit ungewöhnlichen Strukturen isoliert. Die Analyse der Kohlenstoffgerüste dieser Divergolide A–D (1–4) deutet auf eine gemeinsame Biogenese hin, ausgehend von einer linearen Vorstufe, die verschiedene polycyclische aufbauen kann. Diese beachtliche Flexibilität in Biosynthese führt zu Metaboliten, sich ihren Bioaktivitätsprofilen unterscheiden und antibakterielle...

ABSTRACT Scrub typhus is a potentially lethal infection caused by the obligate intracellular bacterium Orientia tsutsugamushi . Reports on emergence of doxycycline-resistant strains highlight urgent need to develop novel antiinfectives against scrub typhus. Corallopyronin A (CorA) α-pyrone compound synthesized myxobacterium Corallococcus coralloides that was characterized as noncompetitive inhibitor switch region bacterial RNA polymerase (RNAP). We investigated antimicrobial action CorA...

Fungal infections pose a great threat to public health and there are only four main types of antifungal drugs, which often limited with toxicity, drug-drug interactions antibiotic resistance. Streptomyces is an important source antibiotics, represented by the clinical drug amphotericin B. Here we report discovery alligamycin A (1) as compound from rapamycin-producer iranensis through genome-mining, genetics natural product chemistry approaches. Alligamycin harbors unique chemical scaffold 13...

Low-molecular-weight natural products from microbes are indispensable in the development of potent drugs. However, their biological roles within an ecological context often remain elusive. Here, we shed light on eukaryotic microorganisms that have ability to transition single cells multicellular organisms: social amoebae. These eukaryotes harbor a large number polyketide biosynthetic genes genomes, yet virtually none corresponding can be isolated or characterized. Using complementary...

Abstract We characterized two key biosynthetic intermediates of the intriguing rubterolone family (tropolone alkaloids) that contain a highly reactive pyran moiety (in equilibrium with hydrolyzed 1,5‐dione form) and undergo spontaneous pyridine formation in presence primary amines. exploited intrinsic reactivity isolated several new derivatives, which unique thiazolidine moiety. Three derivatives were chemically modified fluorescence biotin tags using peptide coupling click reaction....

A tree's travel companion: fungal endophyte (Fusarium incarnatum) isolated from a viviparous propagule (embryo) of mangrove tree produces typical plant defense oxylipins. Stable-isotope labeling experiments revealed that the biosynthesizes coriolic acid, didehydrocoriolic and an epoxy fatty acid derived linoleic by process involving Δ15-desaturation 13-lipoxygenation. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but...

Abstract The structures of the title compounds point to a common biosynthetic precursor.

Abstract Cyclic lipopeptides are substances with a high potential to act as antimicrobial agents. Jagaricin, produced by Janthinobacterium agaricidamnosum DSM 9628 and discovered in 2012, is new member of this class promising antifungal properties. However, further experiments investigate future applications and/or conduct chemical derivatization change properties toxicity impossible due the limited access jagaricin. Besides jagaricin concentration at end fermentation process, suitable...

Ansamycins, represented by the antituberculosis drug rifamycin, are an important family of natural products. To obtain new ansamycins, Streptomyces rapamycinicus IMET 43975 harboring ansamycin biosynthetic gene cluster was fermented in a 50 L scale, and subsequent purification work led to isolation five known four analogues, where hygrocin W (2) belongs benzoquinonoid other three hygrocins, hygrocins X–Z (6–8), seco-hygrocins. The structures ansamycins (1–8) were determined analysis...

Abstract Soil microbiota can confer fitness advantages to plants and increase crop resilience drought other abiotic stressors. However, there is little evidence on the mechanisms correlating a microbial trait with plant stress tolerance. Here, we report that class of Streptomyces effectively alleviates salinity by producing new spiroketal polyketide pteridic acid H (1) its isomer F (2). The bifunctional (pta) biosynthetic gene cluster (BGC), which also responsible for biosynthesis known...

Abstract Fungal infections pose a great threat to public health and there are limited antifungal medicaments. Streptomyces is an important source of antibiotics, represented by the clinical drug amphotericin B. The rapamycin-producer iranensis harbors unparalleled Type I polyketide synthase, which codes for novel macrolide alligamycin A ( 1 ), structure was confirmed NMR, MS, X-ray crystallography. Alligamycin undescribed carbon skeleton with 13 chiral centers, featuring (β-lactone moiety,...