A5039045211- Radiation Therapy and Dosimetry
- Advanced Radiotherapy Techniques
- Effects of Radiation Exposure
- DNA Repair Mechanisms
- Particle accelerators and beam dynamics
- Radiation Detection and Scintillator Technologies
- PARP inhibition in cancer therapy
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Ion-surface interactions and analysis
- Image Processing Techniques and Applications
- Head and Neck Cancer Studies
- Molecular Biology Techniques and Applications
- Radiation Effects in Electronics
- Particle Detector Development and Performance
- DNA and Nucleic Acid Chemistry
- Plant Genetic and Mutation Studies
- Mass Spectrometry Techniques and Applications
- Management of metastatic bone disease
- Medical Imaging Techniques and Applications
- Nonmelanoma Skin Cancer Studies
- Digital Holography and Microscopy
- Integrated Circuits and Semiconductor Failure Analysis
- Carcinogens and Genotoxicity Assessment
- Particle Accelerators and Free-Electron Lasers
Queen Square Radiosurgery Centre
2025
Universität der Bundeswehr München
2017-2024
Abstract FLASH-radiotherapy may provide significant sparing of healthy tissue through ultra-high dose rates in protons, electrons, and x-rays while maintaining the tumor control. Key factors for FLASH effect might be oxygen depletion, immune system, irradiated blood volume, but none could fully confirmed yet. Therefore, further investigations are necessary. We investigated protective (tissue sparing) proton treatment using an in-vivo mouse ear model. The right ears Balb/c mice were with 20...
DNA double strand breaks (DSB) play a pivotal role for cellular damage, which is hazard encountered in toxicology and radiation protection, but also exploited e.g. eradicating tumors therapy. It still debated whether how far clustering of such lesions leads to an enhanced severity induced damage. Here we investigate - using focused spots ionizing as damaging agent the spatial extension lesion patterns causing cell inactivation. We find that damage on both nm µm scale inactivation compared...
Stereotactic radiosurgery with established systems like the Gamma Knife and CyberKnife (Accuray Inc., Madison, WI, USA) is a well-characterized treatment concept. The novel ZAP-X® platform (ZAP Surgical Systems San Carlos, CA, for vault-free, self-shielding gyroscopic (GRS) promises high plan quality due to advantageous beam properties. However, clinically usable workspace in GRS reduced potential collisions spacious headrest. A "conformal" headrest was introduced December 2023 remedy this,...
Proton radiotherapy using minibeams of sub-millimeter dimensions reduces side effects in comparison to conventional proton therapy due spatial fractionation. Since the widen with depth, homogeneous irradiation a tumor can be ensured by adjusting beam distances size and depth maintain control as therapy. The inherent advantages protons photons like limited range that prevents dosage distal tissues are maintained even exploited for interlacing from different directions. A first animal study...
Purpose Proton minibeam radiotherapy using submillimeter beam dimensions allows to enhance tissue sparing in the entrance channel by spatial fractionation additionally advantageous proton depth dose distribution. In channel, leads reduced side effects compared conventional therapy. The sized beams widen with due small angle scattering and enable therefore, contrary x‐ray microbeam radiation therapy ( MRT ), homogeneous irradiation of a tumor. minibeams can either be applied as planar or...
Side effects caused by radiation are a limiting factor to the amount of dose that can be applied tumor volume. A novel method reduce side in radiotherapy is use spatial fractionation, which pattern sub-millimeter beams (minibeams) spare healthy tissue. In order determine skin reactions dependence single beam sizes, relevant for spatially fractionated approaches, pencil submillimeter 6 millimeter size were BALB/c mice ears at Small Animal Radiation Research Platform (SARRP) with plateau 60...
The concept of spatial fractionation in radiotherapy was developed for better sparing normal tissue the entrance channel radiation. Spatial utilizing proton minibeam (pMBRT) promises to be advantageous compared X-ray minibeams due higher dose conformity at tumor. Preclinical vivo experiments conducted with pMBRT mouse ear models or rat brains support prospects, but research about radiobiological mechanisms and search adequate application parameters delivering most beneficial therapy is still...
The use of different scoring systems for radiation-induced toxicity limits comparability between studies. We examined dose-dependent tissue alterations following hypofractionated X-ray irradiation and evaluated their as criteria. Four dose fractions (0, 5, 10, 20, 30 Gy/fraction) were applied daily to ear pinnae. Acute effects (ear thickness, erythema, desquamation) monitored 92 days after fraction 1. Late (chronic inflammation, fibrosis) the presence transforming growth factor beta 1...
ABSTRACT Nucleoli have attracted interest for their role as cellular stress sensors and potential targets cancer treatment. The effect of DNA double-strand breaks (DSBs) in nucleoli on rRNA transcription nucleolar organisation appears to depend the agent used introduce DSBs, DSB frequency presence (or not) DSBs outside nucleoli. To address controversy, we targeted with carbon ions at ion microbeam SNAKE. Localized irradiation 1–100 per point (about 0.3–30 Gy nucleus) did not lead overall...
Proton minibeam radiotherapy (pMBRT) is a spatial fractionation method using sub-millimeter beams at center-to-center (ctc) distances of few millimeters to widen the therapeutic index by reduction side effects in normal tissues. Interlaced minibeams from two opposing or four orthogonal directions are calculated minimize effects. In particular, heterogeneous dose distributions applied tumor investigated evaluate optimized sparing capabilities tissues close surrounding. A 5 cm thick considered...
Ion-radiation-induced DNA double-strand breaks can lead to severe cellular damage ranging from mutations up direct cell death. The interplay between the chromatin surrounding and proteins responsible for recognition repair determines efficiency outcome of repair. is organized in three major functional compartments throughout interphase: territories, interchromatin compartment, perichromatin lying between. In this study, we perform correlation analysis using super-resolution STED images...
Purpose: High doses of ionizing radiation in radiotherapy can elicit undesirable side effects to the skin. Proton minibeam (pMBRT) may circumvent such limitations due tissue-sparing observed at macro scale. Here, we mapped DNA damage dynamics a 3D tissue context sub-cellular level. Methods: Epidermis models were irradiated with planar proton minibeams 66 µm, 408 µm and 920 widths inter-beam-distances 2.5 mm an average dose 2 Gy using scanning-ion-microscope SNAKE Garching, GER. γ-H2AX +...
The prerequisite of any radiation therapy modality (X-ray, electron, proton, and heavy ion) is meant to meet at least a minimum prescribed dose location in the tumor for best control. In addition, there also an upper limit within according International Commission on Radiation Units (ICRU) recommendations order spare healthy tissue as well possible. However, may profit from lower side effects when waving this allowing larger heterogeneous deposition tumor, but maintaining level, particularly...
The fundamental basis in the development of novel radiotherapy methods is in-vitro cellular studies. To assess different endpoints reactions to irradiation like proliferation, cell cycle arrest, and death, several assays are used radiobiological research as standard methods. For example, colony forming assay investigates survival Caspase3/7-Sytox death. major limitation these analysis at a fixed timepoint after irradiation. Thus, not much known about before or performed. Additionally, need...
Abstract In minibeam therapy, the sparing of deep-seated normal tissue is limited by transverse beam spread caused small-angle scattering. Contrary to proton minibeams, helium or carbon minibeams experience less deflection, which potentially reduces side effects. To verify this potential, an irradiation facility for preclinical and clinical studies needed. This manuscript presents a concept based on LINAC design conventional therapy. A quadrupole triplet focuses submillimeter minibeams....
Mitochondria, organelles of the cytoplasm, are power plants cell and thus their function is important for survival cells. Mitochondria known to depolarize after targeted irradiation, but effects on cells still unclear. The aim this work investigate mitochondrial depolarization growth We performed irradiation with 55 MeV C5+-ions at ion-microbeam SNAKE 14 MV tandem accelerator in Garching near Munich, a beam spot size ∼1 µm. Approx. 6% area was irradiated 74 5,120 carbon ions homogenously...
High-linear energy transfer (LET) radiation, such as heavy ions is associated with a higher relative biological effectiveness (RBE) than low-LET photons. Irradiation low- and high-LET particles differ in the interaction cellular matter therefore spatial dose distribution. When single particle interacts matter, it results doses of up to thousands gray (Gy) locally concentrated around ion trajectory, whereas mean averaged over target, cell nucleus only range Gy. DNA damage accumulates this...
Abstract The human body is constantly exposed to ionizing radiation of different qualities. Especially the exposure high-LET (linear energy transfer) particles increases due new tumor therapy methods using e.g. carbon ions. Furthermore, upon accidents, a mixture quality adding up exposure. Finally, long-term space missions such as mission mars pose great challenges dose assessment an astronaut was to. Currently, DSB counting γH2AX foci used exact dosimetric measure for individuals. Due size...
DNA repair eukaryotic cells have additional protective mechanisms that avoid uncontrolled interaction of different parts the chromatin and damaged regions. Key factors here are regulation density mobility. The 4D (temporal spatial) organization is controlling this security barrier by regulating accessibility genes, flexibility DNA, its ability to move inside nucleus. How involved in upon radiation damage until now rarely known but an important part understand enhanced effectiveness high...