A5022601675- Melanoma and MAPK Pathways
- Cancer, Lipids, and Metabolism
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Metabolomics and Mass Spectrometry Studies
- Immunotherapy and Immune Responses
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- RNA modifications and cancer
- Lipid metabolism and biosynthesis
- Olfactory and Sensory Function Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Ubiquitin and proteasome pathways
- Neuroendocrine regulation and behavior
- Advanced Biosensing Techniques and Applications
- Fibroblast Growth Factor Research
- Neuroblastoma Research and Treatments
- Mathematical Biology Tumor Growth
- Pulmonary Hypertension Research and Treatments
- Cholesterol and Lipid Metabolism
- CAR-T cell therapy research
German Cancer Research Center
2020-2025
Heidelberg University
2020-2025
DKFZ-ZMBH Alliance
2024
Deutschen Konsortium für Translationale Krebsforschung
2019-2020
Essen University Hospital
2018-2019
Vrije Universiteit Brussel
2019
Heinrich Heine University Düsseldorf
2019
Düsseldorf University Hospital
2019
University of Duisburg-Essen
2019
Ruhr University Bochum
2013-2016
Abstract Increased glycolytic flux is a hallmark of cancer; however, an increasing body evidence indicates that ATP production may be dispensable in cancer, as metabolic plasticity allows cancer cells to readily adapt disruption glycolysis by via oxidative phosphorylation. Using functional genomic screening, we show here liver unique sensitivity toward aldolase A (ALDOA) depletion. Targeting disrupting the catalytic activity ALDOA led severe energy stress and cell cycle arrest murine human...
The specific functions of sensory systems depend on the tissue-specific expression genes that code for molecular sensor proteins are necessary stimulus detection and membrane signaling. Using Next Generation Sequencing technique (RNA-Seq), we analyzed complete transcriptome trigeminal ganglia (TG) dorsal root (DRG) adult mice. Focusing with an level higher than 1 FPKM (fragments per kilobase transcript million mapped reads), detected 12984 in TG 13195 DRG. To analyze gene patterns peripheral...
The detection of external cues is fundamental for human spermatozoa to locate the oocyte in female reproductive tract. This task requires a specific chemoreceptor repertoire that expressed on surface spermatozoa, which not fully identified date. Olfactory receptors (ORs) are candidate molecules and have been attributed be involved sperm chemotaxis chemokinesis, indicating an important role mammalian spermatozoa. An increasing importance has suggested spermatozoal RNA, led us investigate...
Ferroptosis has emerged as an attractive strategy in cancer therapy. Understanding the operational networks regulating ferroptosis may unravel vulnerabilities that could be harnessed for therapeutic benefit. Using CRISPR-activation screens hypersensitive cells, we identify selenoprotein P (SELENOP) receptor, LRP8, a key determinant protecting MYCN-amplified neuroblastoma cells from ferroptosis. Genetic deletion of LRP8 leads to result insufficient supply selenocysteine, which is required...
SREBP2 is a master regulator of the mevalonate pathway (MVP), biosynthetic process that drives synthesis dolichol, heme A, ubiquinone and cholesterol also provides substrates for protein prenylation. Here, we identify as novel substrate USP28, deubiquitinating enzyme frequently upregulated in squamous cancers. Our results show silencing USP28 reduces expression MVP enzymes lowers metabolic flux into this pathway. We binds to mature SREBP2, leading its deubiquitination stabilisation....
PURPOSE Circulating cell-free tumor DNA (ctDNA) reflects the heterogeneous spectrum of tumor-specific mutations, especially in systemic disease. We validated plasma-based assays that allow dynamic quantitative detection ctDNA as a prognostic biomarker for load and prediction therapy response melanoma. MATERIALS METHODS analyzed plasma-derived from large training cohort (n = 96) patients with advanced-stage melanoma, BRAF V600E NRAS Q61 driver mutations well TERT C250T C228T promoter...
Abstract Melanoma is a highly plastic tumor characterized by dynamic interconversion of different cell identities depending on the biological context. cells with high expression H3K4 demethylase KDM5B (JARID1B) rest in slow-cycling, yet reversible persister state. Over time, can promote rapid repopulation equilibrated heterogeneity. The cellular identity has not been studied so far, missing an important state-directed treatment opportunity melanoma. Here, we have established...
G protein-coupled receptors (GPCRs) transduce external chemical cues into intracellular signals and are involved in a plethora of physiological processes, but knowledge regarding the function these spermatozoa is limited. In present study, we performed RNA-Seq analyzed expression all GPCRs except olfactory human spermatozoa. We revealed up to 223 different GPCR transcripts (FPKM > 0.1) identified GPR18, newly described cannabinoid receptor, together with GPR137 GPR135, as one three most...
The TP53 gene is mutated in approximately 30% of all breast cancer cases. Adipocytes and preadipocytes, which constitute a substantial fraction the stroma normal mammary tissue tumors, undergo transcriptional, metabolic, phenotypic reprogramming during development play an important role tumor progression. We report here that p53 loss cells facilitates inducing them to acquire unique transcriptional metabolic program combines impaired adipocytic differentiation with augmented cytokine...
Abstract Natural products (NPs) are an important inspirational source for developing drugs and chemical probes. In 1999, the group of Ōmura reported constitutional elucidation zelkovamycin. Although largely unrecognized so far, this NP displays structural similarities as well differences to argyrin family, a class peptidic NPs with promising anticancer activities diverse mode‐of‐action at molecular level. By combination structure experiments, first total synthesis zelkovamycin bioassays,...
Abstract Despite the continued success of advanced cancer therapies, hepatocellular carcinoma (HCC) represents a substantial challenge to current treatment modalities that are not sufficiently effective for management disease. Metabolic reprogramming is hallmark and many oncogenic signaling pathways impact on cellular metabolism. Here, we investigating changes in metabolism different oncogene-driven mouse models liver identify selective metabolic vulnerabilities associated with HCC could be...
Abstract Melanoma is a highly plastic tumor characterized by dynamic interconversion of different cell identities depending on the biological context. For example, melanoma cells with high expression H3K4 demethylase KDM5B (JARID1B) rest in slow-cycling, yet reversible persister state. Over time, can promote rapid repopulation equilibrated heterogeneity. The cellular identity has not been studied so far, missing an important state-directed treatment opportunity melanoma. Here, we have...
Introduction Melanoma progression is often associated with the growth of anatomically critical metastases that require immediate therapy response, e.g. in brain. In such situations, systemic drugs are sequentially combined radiotherapy. However, there a lack clinical and preclinical studies systematically examine potential effects therapy-timing on treatment efficacy radio-cross-resistance melanoma. Material methods We established an experimental platform comprising cell culture mouse...
Introduction Melanoma is an aggressive tumour with a median survival of only 6–12 month after occurrence metastasis. In detail, one key factor drug resistance mechanism in melanoma pathogenesis cell heterogeneity. A defined subpopulation slow cycling cells marked by high histone demethylase JARID1B expression are therapy resistant and most likely responsible for repopulation. Moreover, phenotype described to modulate metabolism under targeted therapy. Therefore, the aim my thesis...
9548 Background: The field of liquid biopsy provides a promising alternative to standard tissue biopsies. Previous work has shown that plasma circulating cell-free DNA (ctDNA) can reflect the heterogeneous spectrum mutations in cancer including metastatic melanoma. Our project aimed establish and statistically validate plasma-based assays for tumour load therapy monitoring Methods: On large cohort stage III IV melanoma patients (N = 96) who received signalling targeted or immune checkpoint...