A5078505214- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Barrier Structure and Function Studies
- RNA regulation and disease
- Neuroendocrine regulation and behavior
- Trace Elements in Health
- Alzheimer's disease research and treatments
- Child and Adolescent Psychosocial and Emotional Development
- Iron Metabolism and Disorders
- Neuroscience of respiration and sleep
- Photoreceptor and optogenetics research
- RNA and protein synthesis mechanisms
- Functional Brain Connectivity Studies
- MicroRNA in disease regulation
- RNA Research and Splicing
- Heat shock proteins research
- S100 Proteins and Annexins
- Infant Health and Development
- Hemoglobinopathies and Related Disorders
- Neurogenesis and neuroplasticity mechanisms
- Neurological Disease Mechanisms and Treatments
- Connexins and lens biology
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- Epilepsy research and treatment
University of Lausanne
2022-2023
Centro Universitário Filadélfia
2023
Centre Interdisciplinaire de Recherche en Biologie
2017-2022
Inserm
2017-2022
Collège de France
2017-2022
Université Paris Sciences et Lettres
2017-2022
Centre National de la Recherche Scientifique
2017-2022
Sorbonne Université
2017-2022
Montavid Thermodynamic Research Group
2019
Abstract Astrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate functions as well homeostasis interface. To date, astroglial mechanisms underlying these have been poorly addressed. Here we demonstrate a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, pool bound to ribosomes, endfeetome, primarily encodes for secreted membrane proteins. detected nascent protein synthesis Finally, determined...
Local translation is a conserved mechanism conferring cells the ability to quickly respond local stimuli. In brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet specificity regulation of astrocyte remain unknown. We study hippocampal perisynaptic astrocytic (PAPs) show that they contain machinery for translation. Using refined immunoprecipitation technique, we characterize entire pool ribosome-bound mRNAs PAPs compare with one...
Astrocytes are morphologically complex and use local translation to regulate distal functions. To study the distribution of mRNA in astrocytes, we combined detection via situ hybridization with immunostaining astrocyte-specific intermediate filament glial fibrillary acidic protein (GFAP). mRNAs at level GFAP-immunolabelled astrocyte somata, large fine processes were analysed using AstroDot, an ImageJ plug-in R package AstroStat. Taking characterization encoding GFAP-α GFAP-δ isoforms as a...
Translation of distally localized mRNAs is an evolutionary mechanism occurring in polarized cells. It has been observed astrocytes, whose processes contact blood vessels and synapses. Here, we describe a protocol for the purification entire pool ribosome-bound perisynaptic astrocytic (PAPs). Our procedure combines preparation synaptogliosomes with refined translating ribosome affinity technique. This approach can be used any brain region to probe physiological relevance local translation...
Abstract Astrocytes are thought to play a crucial role in brain iron homeostasis. How they accomplish this regulation vivo is unclear. In recent transcriptomic analysis, we showed that polysomal Ftl1 and Fth1 mRNAs, encoding the ferritin light (Ftl) heavy (Fth) chains assemble into ferritin, critical complex for storage reduction, enriched perisynaptic astrocytic processes as compared soma. These data suggested translation plays specific at interface tighly regulated by local translation....
Abstract Cells with a complex shape often use mRNA distribution and local translation to regulate distal functions. These mechanisms have recently been described in astrocytes, the processes of which contact functionally modulate neighbouring synapses blood vessels. In order study we developed three-dimensional histological method that combines detection via situ hybridization immunostaining astrocyte-specific intermediate filament glial fibrillary acidic protein (GFAP). Three-dimensional...
Abstract Local translation is a conserved molecular mechanism conferring cells the ability to quickly respond local stimuli. It not only permits with complex morphology bypass somatic protein synthesis and transport, but also contributes locally establishment of functional polarity. In brain, has been extensively studied in neurons recently reported astrocytes, whose fine processes contact both blood vessels synapses. Yet specificity regulation astrocyte remain unknown. Here, we hippocampal...
Abstract Parental behaviours are essential to secure survival and wellbeing of newborns1. Concomitantly, parenting also limits negative affective states in adults, which emerge when the coping with excessive neonatal distress becomes a challenge1–5. Whether neural circuits that process affect orchestrate components parental remains, however, unknown. Here, we identify functional transcriptional signatures neurons emotion center lateral habenula receiving bed nucleus stria terminalis...
Abstract Astrocytes are thought to play a crucial role in brain iron homeostasis. How they accomplish this regulation vivo remains unclear. In recent transcriptomic analysis, we showed that polysomal Ftl1 and Fth1 mRNAs, encoding the ferritin light (Ftl) heavy (Fth) chains assemble into ferritin, critical complex for storage reduction, enriched perisynaptic astrocytic processes as compared soma. These data suggested translation plays specific at interface is tighly regulated by local...