A5001010969- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolism and Genetic Disorders
- Diet and metabolism studies
- Nicotinic Acetylcholine Receptors Study
- Epilepsy research and treatment
- Stroke Rehabilitation and Recovery
- Biochemical effects in animals
- Photoreceptor and optogenetics research
- CRISPR and Genetic Engineering
- Caveolin-1 and cellular processes
- Ion channel regulation and function
- Neural dynamics and brain function
- RNA and protein synthesis mechanisms
- Neuroscience of respiration and sleep
- Retinal Development and Disorders
- Anesthesia and Neurotoxicity Research
- RNA regulation and disease
- Neurogenesis and neuroplasticity mechanisms
- Spinal Cord Injury Research
- French Urban and Social Studies
- Cerebral Palsy and Movement Disorders
- RNA Research and Splicing
- Agriculture and Rural Development Research
Centre National de la Recherche Scientifique
2020-2025
Collège de France
2017-2025
Centre Interdisciplinaire de Recherche en Biologie
2016-2025
Inserm
2018-2025
Université Paris Sciences et Lettres
2018-2025
Sorbonne Université
2020-2023
Université Sorbonne Nouvelle
2020
École Pratique des Hautes Études
2016-2017
Pannexin-1 channel inhibition blocks seizure activity in brain slices from epileptic patients and reduces spontaneous seizures a mouse model of pharmacoresistant epilepsy.
How astrocytes close a critical period During the visual period, brain circuits are rewired to adjust sensory input. Closure of stabilizes circuits. Looking at development in mouse cortex, Ribot et al. found that increase their expression gap junction channel subunit connexin 30, which turn inhibits matrix-degrading enzyme (see Perspective by Kofuji and Araque). As matrix stabilizes, inhibitory interneurons mature, unusual flexibility comes an end. Science , abf5273, this issue p. 77 ; see...
Presynaptic glutamate replenishment is fundamental to brain function. In high activity regimes, such as epileptic episodes, this process thought rely on the glutamate-glutamine cycle between neurons and astrocytes. However presence of an astroglial glutamine supply, well its functional relevance in vivo healthy remain controversial, partly due a lack tools that can directly examine transfer. Here, we generated fluorescent probe tracks live cells, which provides direct visual evidence...
Local translation is a conserved mechanism conferring cells the ability to quickly respond local stimuli. In brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet specificity regulation of astrocyte remain unknown. We study hippocampal perisynaptic astrocytic (PAPs) show that they contain machinery for translation. Using refined immunoprecipitation technique, we characterize entire pool ribosome-bound mRNAs PAPs compare with one...
Throughout the brain, astrocytes form networks mediated by gap junction channels that promote activity of neuronal ensembles. Although their inputs on information processing are well established, how molecular shape network patterns remains unclear. Here, using astroglial connexin-deficient mice, in which disconnected and bursting abnormal, we show astrocyte strengthen via dynamic regulation extracellular potassium levels, independently glutamate homeostasis or metabolic support. Using a...
Astrocytes crucially contribute to synaptic physiology and information processing. One of their key characteristics is express high levels connexins (Cxs), the gap junction-forming protein. Among them, Cx30 displays specific properties since it postnatally expressed dynamically upregulated by neuronal activity modulates cognitive processes shaping network activities, as recently shown in knockout mice. However, remains unknown whether local selective upregulation postnatal astrocytes within...
Abstract Spasticity, a prevalent motor issue characterized by network hyperexcitability, causes pain and discomfort, with existing treatments offering limited relief. While past research has focused on neuronal factors, the role of astrocytes in spasticity been overlooked. This study explores potential restoring astrocytic potassium (K+) uptake to reduce following spinal cord injury (SCI). Astrocytes buffer extracellular K+ via Kir4.1 channels, preventing hyperexcitability. Following SCI,...
Abstract Epilepsy is a neurological condition that affects 1% of the world population. Conventional treatments epilepsy use drugs targeting neuronal excitability, inhibitory or excitatory transmission. Yet, one third patients presents an intractable form and fails to respond pharmacological anti-epileptic strategies. The ketogenic diet well-established non-pharmacological treatment has been proven be effective in reducing seizure frequency pharmaco-resistant patients. This dietary solution...
The regulation of translation in astrocytes, the main glial cells brain, remains poorly characterized. We developed a high-throughput proteomics screen for polysome-associated proteins astrocytes and focused on ribosomal protein receptor activated C kinase 1 (RACK1), critical factor translational regulation. In astrocyte somata perisynaptic astrocytic processes (PAPs), RACK1 preferentially binds to number mRNAs, including Kcnj10, encoding inward-rectifying potassium (K+) channel Kir4.1. By...
Astroglial networks closely interact with neuronal populations, but their functional contribution to representation of sensory information remains unexplored. The superior colliculus (SC) integrates multi-sensory by generating distinct spatial patterns responses specific stimulation. Here, we report that astrocytes from the mouse SC form extensive in retinorecipient layer compared visual cortex. This strong astroglial connectivity relies on high expression gap-junction proteins. Genetic...
Astroglial release of molecules is thought to actively modulate neuronal activity, but the nature, pathway, and cellular targets these neuroactive are still unclear. Pannexin 1, expressed by neurons astrocytes, form nonselective large pore channels that mediate extracellular exchange molecules. The functional relevance has been mostly studied in brain tissues, without considering their specific role different cell types, or neurons. Thus, our knowledge astroglial pannexin 1 regulation its...
Abstract Local translation is a conserved molecular mechanism conferring cells the ability to quickly respond local stimuli. It not only permits with complex morphology bypass somatic protein synthesis and transport, but also contributes locally establishment of functional polarity. In brain, has been extensively studied in neurons recently reported astrocytes, whose fine processes contact both blood vessels synapses. Yet specificity regulation astrocyte remain unknown. Here, we hippocampal...
Summary paragraph Brain postnatal development is characterized by critical periods of experience-dependent remodeling 1,2 . Termination these intense plasticity associated with settling neuronal circuits, allowing for efficient information processing 3 Failure to end thus results in neurodevelopmental disorders 4,5 Yet, the cellular processes defining timing developmental remain unclear. Here we show mouse visual cortex that astrocytes control closure period. We uncover a novel underlying...
Abstract Presynaptic glutamate replenishment is fundamental to brain function. In high activity regimes, such as epileptic episodes, this process thought rely on the glutamate-glutamine cycle between neurons and astrocytes. However presence of an astroglial glutamine supply, well its functional relevance in vivo healthy remain controversial, partly due a lack tools that can directly examine transfer. Here, we generated novel fluorescent probe tracks live cells, which provided direct visual...
Summary The regulation of translation in astrocytes, the main glial cells brain, remains poorly characterized. We developed a high-throughput proteomic screen for polysome-associated proteins astrocytes and focused on ribosomal protein receptor activated C kinase 1 (RACK1), critical factor translational regulation. In astrocyte somata perisynaptic astrocytic processes (PAPs), RACK1 preferentially bound to number mRNAs, including Kcnj10 , encoding inward rectifying potassium (K + ) channel...