Anaerobic digestion (AD) is a microbially-driven process enabling energy production. Microorganisms are the core of anaerobic digesters and play an important role in succession hydrolysis, acidogenesis, acetogenesis, methanogenesis processes. The diversity participating microbial communities can provide new information on digester performance for biomass valorization biofuel In this study systems were used, operating under mesophilic conditions that realized biodegradation processes waste...

Anaerobic co-digestion of waste wheat straw and horse manure in two steps was revealed as a promising option for renewable energy production the form hydrogen methane. Addition cooking oils, disposal which could cause damage to health or environment, third substrate digestion, is suggested an approach not only help handle increasing volume food worldwide but also improve process performance. In present study, oil, concentration 5%, appeared be positive modulator anaerobic digestion with did...

Taking into account the rising trend of incidence cancers various organs, effective therapies are urgently needed to control human malignancies. However, almost all chemotherapy drugs currently on market cause serious side effects. Fortunately, several studies have shown that some non-toxic biological macromolecules, including algal polysaccharides, possess anti-cancer activities or can increase efficacy conventional drugs. Polysaccharides characteristic secondary metabolites many algae. The...

New bacterial strain BN66 was isolated by selective enrichment, identified as Bacillus cereus and proved to degrade crude oil, together with biosurfactant synthesis. Free cryogel immobilized cells were involved in a oil degradation process. The studied degraded 93% of the aliphatic fraction for 48 h. We two types cryogels synthesized from high molar mass polyacrylamide or acrylamide precursors explored capability possibility re-use preparations. Reusability tests revealed that ability stable...

Anaerobic digestion (AD) is a widespread biological process treating organic waste for green energy production. In this study, wheat straw and corn stalks without any harsh preliminary treatment were collected as renewable source to be employed in laboratory-scale digester produce biogas/biomethane. Processes parameters of temperature, pH, total solids, volatile solid, concentration fatty acids (VFA), cellulose concentration, followed. The volume biogas produced was measured. impact loading...

Economic development increases and brings about issues such as the secure supply of food in a sustainable way. Phytases are enzymes catalyzing phytate hydrolysis to release phosphorus an inorganic form. Animal feeds could be supplemented with bacterial phytases increase their micronutrients bioavailability. To best our knowledge, this is first report on purification characterization alkalophilic phytase from Cobetia marina. The purified newly isolated halophilic marina strain 439 appears...

This study introduces a novel mathematical model characterizing the anaerobic co-digestion of wheat straw and waste algal biomass for hydrogen methane production, implemented in two-stage bioreactor system. Co-digestion can be tool to increase biogas production utilizing difficult-to-digest organic by introducing easily degradable substrates. Two continuous operational regimes, with loading rates 50 g/L 33 g/L, were employed generate experimental datasets parameterization validation,...

Background/Aim: A number of biologically active substances were proved as an alternative to conventional anticancer medicines. The aim the study is in vitro investigation activity mono- and di-Rhamnolipids (RL-1 RL-2) against human breast cancer. Additionally, combination with Cisplatin was analyzed. Materials Methods: Breast cell lines (MCF-10A, MCF-7 MDA-MB-231) treated RLs Cisplatin. viability analyzed using MTT assay, autophagy performed via acridine orange staining. Results: In contrast...

The main purpose of this study was to identify the microbial communities (bacterial, archaeal and fungal) in a two-stage system anaerobic bioreactors for production hydrogen methane from waste substrate-corn steep liquor. Wastes food industry are valuable resources with potential biotechnological because their high organic matter contents. In addition, methane, volatile fatty acids, reducing sugars cellulose content monitored. Two-stage biodegradation processes were performed by populations...

In this paper, a new Newton-based extremum-seeking control for dynamic systems is proposed using Kalman filter gradient and Hessian estimation as well stochastic perturbation signal with time-varying amplitude. The obtained based Newton (KFNESC) makes it possible to accelerate the convergence extremum attenuate steady-state oscillations. oscillation attenuation properties of closed-loop system KFNESC are considered, applied two-stages anaerobic digestion process in order maximize hydrogen...

This study utilized a renewable energy source, agricultural waste, in anaerobic digestion (AD) at appropriate conditions to obtain biogas and biomethane as an carrier. Maize stalks underwent ultrasound (US) pre-treatment for better accessibility microorganisms, lignocelluloses have stable structure, insoluble water resist both mechanical enzymatic attack. The digestate after process was used cultivation of algae adsorption with activated carbon clarification. Photosynthetic microalgae...

Abstract In this work the application of hybrid materials, containing TEOS as source SiO2 and k-carrageenan in different percentage, synthesized by sol-gel method at room temperature was studied. They were used matrices for entrapment whole Bacillus sp. UG-5B cells, producers thermostable nitrilase. The effect surface area size quantity pores materials on enzyme activity evaluated. process biodegradation concentrations toxic, potentially carcinogenic mutagenic substrates obtained...

Isolation and characterization of new biologically active substances affecting cancer cells is an important issue fundamental research in biomedicine. Trehalose lipid was isolated from Rhodococcus wratislaviensis strain purified by liquid chromatography. The effect trehalose on cell viability migration, together with colony forming assays, were performed two breast (MCF7-low metastatic; MDA-MB231-high metastatic) one "normal" (MCF10A) lines. Molecular modeling that details the structure...

The interest in microbial pectinases is increasing due to their use the biotechnological and food industries response global demand. In this study, among 22 halophilic bacterial strains screened, 20 showed pectinase activity. highest activity was measured for Virgibacillus salarius strain 434. As production meets many parameter optimization constraints, optimal conditions maximizing yield of synthesized using investigated were followed. Enzyme purification accomplished at up 11.5-fold via...

The main purpose of the present work is sol-gel synthesis and structure hybrid nanomaterials as matrices for two types cells, producing hydrolytic enzymes. effect different percent algal polysaccharide included in them on activity fungal bacterial cells was investigated.The were synthesized from tetraethylortosilicate (TEOS) a silicon precursor heteropolysaccharide (AHPS) red microalga Dixonella grisea an organic part. these investigated using methods: FT-IR, XRD, BET-Analysis, EDS, SEM AFM....

Abstract This work reports the sol-gel synthesis of silica hybrids. We determined effect type and quantity precursors organic compounds on resulting structure, surface area, nanostructure design size, potential applications. The structure synthesized hybrids was analyzed using FT-IR, XRD, BET-Analysis, SEM, AFM. demonstrate immovilization whole living thermophilic bacterial cells with cyanocompound degradation activity in hybrid biomaterials by entrapment, chemical binding, adsorption.

Production of a rhamnolipid biosurfactant by cells Pseudomonas aeruginosa strain BN10 immobilized into poly(ethylene oxide) (PEO) and polyacrylamide (PAAm) cryogels was investigated under semicontinuous shake fl ask conditions compared to secretion free cells. The synthesis followed over 9 cycles operation the system, each cycle comprising 7 days at ambient temperature neutral pH. Type quantity carrier were optimized for production. highest yield 4.6 g l -1 obtained in 6 th system with 3 PEO...