A5068914630- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Cancer-related gene regulation
- RNA modifications and cancer
- Invertebrate Immune Response Mechanisms
- Physiological and biochemical adaptations
- Ion Channels and Receptors
- Alzheimer's disease research and treatments
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Advanced Fluorescence Microscopy Techniques
- Insect Utilization and Effects
- Virus-based gene therapy research
- Genomics and Rare Diseases
- Connective tissue disorders research
- Magnesium in Health and Disease
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Protein Degradation and Inhibitors
- Neuroscience and Neuropharmacology Research
- Silk-based biomaterials and applications
- Animal Behavior and Reproduction
- Kruppel-like factors research
University of Washington
2014-2024
Morgan Stanley Children's Hospital
2023
Columbia University
2020-2023
Cedars-Sinai Medical Center
2023
University of California, San Diego
2019-2022
Columbia University Irving Medical Center
2021-2022
Children’s Institute
2021
Howard Hughes Medical Institute
2019
Seattle University
2019
Union Hospital
2016
As animals grow, many early born structures grow by cell expansion rather than addition; thus growth of distinct must be coordinated to maintain proportionality. This phenomenon is particularly widespread in the nervous system, with dendrite arbors neurons expanding concert their substrate sustain connectivity and receptive field coverage as grow. After rapidly growing establish body wall coverage, dendrites Drosophila class IV arborization (C4da) synchronously substrate, epithelium,...
The limited resolving power of conventional diffraction-limited microscopy hinders analysis small, densely packed structural elements in cells. Expansion (ExM) provides an elegant solution to this problem, allowing for increased resolution with standard microscopes via physical expansion the specimen a swellable polymer hydrogel. Here, we apply, validate, and optimize ExM protocols that enable study Drosophila embryos, larval brains, adult body walls. We achieve lateral ∼70 nm tissues using...
Interactions between epithelial cells and neurons influence a range of sensory modalities including taste, touch, smell. Vertebrate invertebrate epidermal ensheath peripheral arbors somatosensory neurons, nociceptors, yet the developmental origins functional roles this ensheathment are largely unknown. Here, we describe an evolutionarily conserved morphogenetic mechanism for neurites. We found that in Drosophila zebrafish induce formation sheaths, which wrap neurites different types to...
Structural birth defects occur in approximately 3% of live births; most such lack defined genetic or environmental causes. Despite advances surgical approaches, pharmacologic prevention remains largely out reach.
The eukaryotic genome is partitioned into distinct topological domains separated by boundary elements. Emerging data support the concept that several well-established nuclear compartments are ribonucleoprotein condensates assembled through physical process of phase separation. Here, based on our demonstration chemical disruption condensate assembly weakens insulation properties a specific subset (∼20%) topologically associated domain (TAD) boundaries, we report disrupted boundaries...
TRPM3 encodes a transient receptor potential cation channel of the melastatin family, expressed in central nervous system and peripheral sensory neurons dorsal root ganglia. The recurrent substitution TRPM3: c.2509G>A, p.(Val837Met) has been associated with syndromic intellectual disability seizures. In this report, we present clinical molecular features seven previously unreported individuals, identified by exome sequencing, variant global developmental delay. Other shared included...
Chemotherapy induced peripheral neuropathy (CIPN), a side effect of many anti-cancer drugs including the vinca alkaloids, is characterized by severe pain syndrome that compromises treatment in patients. Currently there are no effective treatments for this except reduction drug dose. Existing data supports model associated with CIPN result augmenting function sensory nociceptors but cellular mechanisms underlying effects on neuron not well described. Studies from animal models have suggested...
The identification of novel genetic modifiers age-at-onset (AAO) Alzheimer's disease (AD) could advance our understanding AD and provide therapeutic targets. A previous genome scan for AAO among families affected by early-onset caused the PSEN2 N141I variant identified 2 loci with significant evidence linkage: 1q23.3 17p13.2. Here, we describe fine-mapping these linkage regions, test replication in 6 independent datasets. By signals a single large family, reduced regions to 11% their...
Abstract We report a novel homozygous 49.6 kb deletion of chromosome 18q12.1 involving the last exon DSG3 in dizygotic twins with phenotype consistent acantholytic blistering oral and laryngeal mucosa (ABOLM). The twin siblings presented predominantly friability respiratory mucosa. This is only second literature this unusual autosomal recessive disorder. diagnosis explains mucosal pemphigus‐like disorder without evidence autoimmune dysfunction. exclusion an basis has management implications....
An animal’s skin provides a first point of contact with the sensory environment, including noxious cues that elicit protective behavioral responses. Nociceptive somatosensory neurons densely innervate and intimately interact epidermal cells to receive these cues, however mechanisms by which interactions shape processing inputs is still poorly understood. Here, we identify role for dendrite intercalation between in tuning sensitivity Drosophila larvae mechanical stimuli. In wild-type larvae,...
Here, we investigated the effects of blocking chicken telomerase reverse transcriptase (chTERT) in MDCC-MSB1 cells, using small-hairpin RNAs (shRNAs) to interfere with gene expression.shRNAs specific chTERT mRNA were designed, cloned into DNA plasmid vectors, and transfected cells.The expressed by RNA polymerase machinery cells.mRNA expression cells was detected real-time PCR.After transfection, activity monitored via a modified telomeric repeat amplification protocol assay, cell cycle...
Abstract Interactions between epithelial cells and neurons influence a range of sensory modalities including taste, touch, smell. Vertebrate invertebrate keratinocytes/keratinocyte-like epidermal ensheath peripheral arbors somatosensory neurons, nociceptors, yet the developmental origins functional roles this ensheathment are largely unknown. Here, we describe an evolutionarily conserved morphogenetic mechanism for neurites. We found that in Drosophila zebrafish induce formation sheaths,...
Abstract An animal’s skin provides a first point of contact with the sensory environment, including noxious cues that elicit protective behavioral responses. Nociceptive somatosensory neurons densely innervate and intimately interact epidermal cells to receive these cues, however mechanisms by which interactions shape processing inputs is still poorly understood. Here, we identify role for dendrite intercalation between in tuning sensitivity Drosophila larvae mechanical stimuli. In wild-type...