A5016173115- Metabolomics and Mass Spectrometry Studies
- Disaster Response and Management
- Artificial Intelligence in Healthcare
- Cancer Genomics and Diagnostics
- Sarcoma Diagnosis and Treatment
- Tumors and Oncological Cases
- CAR-T cell therapy research
- Neuroblastoma Research and Treatments
- Virus-based gene therapy research
- Cardiac Arrest and Resuscitation
- Renal and related cancers
- Renal cell carcinoma treatment
- Poisoning and overdose treatments
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Parvovirus B19 Infection Studies
- Acute Lymphoblastic Leukemia research
- Caveolin-1 and cellular processes
- Acute Myeloid Leukemia Research
- RNA Research and Splicing
- Vascular Tumors and Angiosarcomas
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Rabies epidemiology and control
- Soft tissue tumor case studies
- Radiopharmaceutical Chemistry and Applications
AdventHealth Kissimmee
2023
The University of Texas Southwestern Medical Center
2019-2023
Center for Cancer and Blood Disorders
2019-2023
Children's Medical Center
2022-2023
Harold C. Simmons Comprehensive Cancer Center
2021
Cornell University
2017
Weill Cornell Medicine
2017
Manhattan Psychiatric Center
2005
Advantagene (United States)
2004
Baylor College of Medicine
2004
Histiocytic neoplasms are rare hematologic disorders accounting for less than 1% of cancers the soft tissue and lymph nodes. Clinical presentation prognosis these can be highly variable, leading to challenges diagnosis optimal management patients. Treatment often consists systemic therapy, recent studies support use targeted therapies patients with disorders. Observation ("watch wait") may sufficient select mild disease. These NCCN Guidelines Neoplasms include recommendations treatment...
Microbial communities in our built environments have great influence on human health and disease. A variety of been characterized using a metagenomics-based approach, including some healthcare settings. However, there has no study to date that used this approach pre-hospital settings, such as ambulances, an important first point-of-contact between patients hospitals. We sequenced 398 samples from 137 ambulances across the USA shotgun sequencing. analyzed these data explore microbial ecology...
Organotropism results from the functional versatility of metastatic cancer cells to survive and proliferate in diverse microenvironments. This adaptivity can originate clonal variation spreading tumor is often empowered by epigenetic molecular reprogramming cell regulatory circuits. Related organotropic colonization sites are environmentally-sensitive, differential responses therapeutic attack. Accordingly, understanding profile a probing underlying driver mechanisms high clinical...
Infantile myofibromatosis (IM) is an aggressive neoplasm composed of myofibroblast-like cells in children. Although typically localized, it can also present as multifocal disease, which represents a challenge for effective treatment. IM has previously been linked to activating somatic and germline point mutations the PDGFRβ tyrosine kinase encoded by PDGFRB gene. Clinical panel-based targeted tumor sequencing from newborn with revealed novel rearrangement, was reported being unclear...
Abstract Subcutaneous patient-derived xenografts (PDXs) are an important tool for childhood cancer research. Here, we describe a resource of 68 early passage PDXs established from 65 pediatric solid tumor patients. Through genomic profiling paired and patient tumors (PTs), observe low mutational similarity in about 30% the PT/PDX pairs. Clonal analysis these pairs show aggressive PT minor subclone seeds major clone PDX. We evidence that this is more immunogenic likely suppressed by immune...
Patient-derived xenografts (PDX) remain valuable models for understanding the biology and developing novel therapeutics. To expand current PDX of childhood leukemia, we have developed new from Hispanic patients, a subgroup with poorer overall outcome. Of 117 primary leukemia samples obtained, successful engraftment serial passage in mice were achieved 82 (70%). patient engrafted at rate (51/73, 70%) that was similar to non-Hispanic (31/45, 70%). With algorithm remove mouse contamination...
Iron poisoning is a significant and potentially life-threatening condition that commonly encountered in the emergency department. The severity of toxicity based on amount iron ingested, symptoms can range from mild gastrointestinal discomfort to multi-organ failure. Although current guidelines recommend therapy for patients with estimated ingestion >60 mg/kg, most useful laboratory test determine serum level measured at four six hours after ingestion. In this report, we present case...
Primary extrarenal Wilms tumors are rare neoplasms that presumed to arise from metanephric or mesonephric remnants outside of the kidney. Their pathogenesis is debated but has not been studied, and there no reports genomic descriptions tumors. We describe a diffusely anaplastic tumor occurred in lower abdomen upper pelvis 10-year-old boy. In addition clinical, histopathologic, radiologic features, we cytogenetic changes exomic profile tumor. The showed loss suppressor AMER1, chromosome...
Malignant peripheral nerve sheath tumor (MPNST) is an aggressive form of soft-tissue sarcoma (STS) in children. Despite intensive therapy, relatively few children with metastatic and unresectable disease survive beyond three years. RAS pathway activation common MPNST, suggesting MEK inhibition as a targeted but the impact on clinical outcome has been small to date.We conducted preclinical pharmacokinetic (PK) pharmacodynamic studies two inhibitors, trametinib selumetinib, MPNST models...
Cellular plasticity is a hallmark function of cancer, but many the underlying mechanisms are not well understood. We uncover Caveolin-1, scaffolding protein that modulates plasma membrane domain organization, as context-specific regulator survival signaling in Ewing sarcoma (EwS). Single cell analysis reveals distinct subpopulation EwS cells, which highly express surface marker CD99 Caveolin-1. High cells isolated through flow cytometry differ from Low morphology, gene expression, and...
4571 Background: A Phase II clinical trial for newly diagnosed, non-metastatic prostate cancer is ongoing in which adenoviral-HSV thymidine kinase (AdV-tk) + prodrug (valacyclovir) added to standard-of-care radiation and hormonal therapy. This study based on animal models demonstrating increased local systemic anti-tumor efficacy from AdV-tk combined with radiation. Methods: Subjects receive transrectal ultrasound guided intratumoral injection of 5x1011 particles followed by oral...
Rhabdomyosarcoma (RMS) is a childhood sarcoma composed of myoblast-like cells, which suggests defect in terminal skeletal muscle differentiation. To explore potential defects the differentiation program, we searched for mRNA splicing variants genes encoding transcription factors driving lineage commitment and We studied two RMS cases identified altered resulting “skipping” second three exons MYOD1 . RNA-seq data from 42 tumors additional cell lines revealed exon 2 skipping both MYF5 but not...
Abstract Background: Cancer causes significant mortality and morbidity in children. Current therapies are effective but can cause long-term health problems for patients. Development of new relies on faithful preclinical models. Patient-derived xenografts (PDXs) an important tool pre-clinical testing childhood cancer research. It remains incompletely understood how well genomically PDXs recapitulate primary patient tumors (PTs), particularly rare cancers. Method: To characterize the fidelity...