A5056215708- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Marine Biology and Ecology Research
- Forest Ecology and Biodiversity Studies
- Molecular Biology Techniques and Applications
- Legume Nitrogen Fixing Symbiosis
- Insect symbiosis and bacterial influences
- Radiomics and Machine Learning in Medical Imaging
- Genomics and Phylogenetic Studies
- Marine and coastal plant biology
- Entomopathogenic Microorganisms in Pest Control
- Marine Bivalve and Aquaculture Studies
- Muscle Physiology and Disorders
- Plant and animal studies
- Parvovirus B19 Infection Studies
- Neurogenetic and Muscular Disorders Research
- RNA and protein synthesis mechanisms
- Plant Parasitism and Resistance
- Gene expression and cancer classification
- Forensic and Genetic Research
- Aquatic Invertebrate Ecology and Behavior
- Cytomegalovirus and herpesvirus research
- Genomics, phytochemicals, and oxidative stress
- Organ and Tissue Transplantation Research
- Plant and Fungal Interactions Research
Keio University
2004-2025
National Institute for Basic Biology
2013-2023
Ryukoku University
2021
Japan Agency for Marine-Earth Science and Technology
2012-2014
Tokyo University of Marine Science and Technology
2010-2012
Chiba University
2010
Rokko Island Kohnan Hospital
1997
Island Hospital
1997
Kobe City Nishi-Kobe Medical Center
1997
Significance Beetles are successful in the terrestrial ecosystem, which is attributable to, at least partly, their highly sclerotized exoskeleton. Here, we report a bacterial symbiont extremely specialized for underpinning beetle’s hardness. The ancient endosymbiont Nardonella associated with weevils has an small genome devoted to single biological function, tyrosine provisioning, needed insect’s cuticle formation and hardening. Notably, only final step reaction of synthesis pathway...
Mycorrhizal symbiosis is one of the most fundamental types mutualistic plant-microbe interaction. Among many classes mycorrhizae, arbuscular mycorrhizae have general symbiotic style and longest history. However, genomes mycorrhizal (AM) fungi are not well characterized due to difficulties in cultivation genetic analysis. In this study, we sequenced genome AM fungus Rhizophagus clarus HR1, compared sequence with model species R. irregularis, checked for missing genes that encode enzymes...
Arbuscular mycorrhizal fungus (AMF) species are some of the most widespread symbionts land plants. Our much improved reference genome assembly a model AMF, Rhizophagus irregularis DAOM-181602 (total contigs = 210), facilitated discovery repetitive elements with unusual characteristics. R. has only ten or 11 copies complete 45S rDNAs, whereas general eukaryotic tens to thousands rDNA copies. rDNAs highly heterogeneous and lack tandem repeat structure. These findings provide evidence for...
Abstract Elysia chlorotica , a sacoglossan sea slug found off the East Coast of United States, is well-known for its ability to sequester chloroplasts from algal prey and survive by photosynthesis up 12 months in absence food supply. Here we present draft genome assembly E. that was generated using hybrid strategy with Illumina short reads PacBio long reads. The comprised 9,989 scaffolds, total length 557 Mb scaffold N50 442 kb. BUSCO assessment indicated 93.3% expected metazoan genes were...
Abstract Arbuscular mycorrhizal (AM) symbiosis is a mutually beneficial interaction between fungi and land plants promotes global phosphate cycling in terrestrial ecosystems. AM are recognised as obligate symbionts that require root colonisation to complete life cycle involving the production of propagules, asexual spores. Recently, it has been shown Rhizophagus irregularis can produce infection-competent secondary spores asymbiotically by adding fatty acid, palmitoleic acid. Furthermore,...
The sea slug Plakobranchus ocellatus (Sacoglossa, Gastropoda) retains photosynthetically active chloroplasts from ingested algae (functional kleptoplasts) in the epithelial cells of its digestive gland for up to 10 months. While feeding behavior has not been observed natural habitats, two hypotheses have proposed: 1) adult P. uses kleptoplasts obtain photosynthates and nutritionally behaves as a photoautotroph without replenishing kleptoplasts; or 2) it mixotroph (photoautotroph herbivorous...
Some sea slugs sequester chloroplasts from algal food in their intestinal cells and photosynthesize for months. This phenomenon, kleptoplasty, poses a question of how the chloroplast retains its activity without nucleus. There have been debates on horizontal transfer genes to animal To settle arguments, this study reported genome kleptoplastic slug, Plakobranchus ocellatus , found no evidence photosynthetic encoded Nevertheless, it was confirmed that light illumination prolongs life mollusk...
Accurate determination of the evolutionary relationships between genes is a foundational challenge in biology. Homology-evolutionary relatedness-is many cases readily determined based on sequence similarity analysis. By contrast, whether or not two directly descended from common ancestor by speciation event (orthologs) duplication (paralogs) more challenging, yet provides critical information history gene. Since 2009, this task has been focus Quest for Orthologs (QFO) Consortium. The sixth...
The signaling lymphocyte activation molecule (SLAM) is a receptor for morbilliviruses. To understand the recent host range expansion of canine distemper virus (CDV) in carnivores, we determined nucleotide sequences SLAMs various carnivores and generated three-dimensional homology SLAM models. Thirty-four amino acid residues were found candidates binding to CDV on interface carnivore SLAMs. domestic dog (Canis lupus familiaris) similar those other members suborder Caniformia, indicating that...
Arbuscular mycorrhizal fungi (AMF) establish symbiotic relationships with most land plants, mainly for the purpose of nutrient exchange. Many studies have revealed regulation processes in AMF, such as absorption from soil, metabolism and exchange host genes involved. However, spatial within structures comprising each developmental stage is not well understood. Here, we demonstrate structure-specific transcriptome model AMF species, Rhizophagus irregularis. We performed an ultra-low input...
We isolated Burkholderia symbiont strain RPE64 from the bean bug Riptortus pedestris. Analysis of complete 6.96-Mb genome, which consists three chromosomes and two plasmids, will facilitate further understanding insect-microbe symbiosis development pest-control technologies.
Members of the genus Rickettsiella are bacterial pathogens insects and other arthropods. Recently, a novel facultative endosymbiont, "Candidatus viridis," was described in pea aphid Acyrthosiphon pisum, whose infection causes striking host phenotype: red green genetic color morphs exist populations, upon with symbiont, aphids become due to increased production polycyclic quinone pigments. Here we determined complete genome sequence symbiont. The 1.6-Mb circular genome, harboring some 1,400...
Salicylic acid (SA) and jasmonic (JA) regulate plant defense against pathogens herbivores. The SA-JA pathways often interact antagonistically with the induction of one, leading to suppression other. However, effect combined SA/JA concentrations on responses is unknown. Here, we show transcriptional specific combinations SA JA develop transcriptomic biomarkers estimate response states. Combinatorial treatments eight JA, large-scale transcriptome analysis, revealed 43 different expression...
Sacoglossan sea slugs retain the chloroplasts from food algae in their cells (kleptoplasty) and obtain photosynthetic products, but capability of kleptoplasty differs among species. One evolutionary hypothesis for is that family Volvatellidae ancestral sacoglossan lineage which arose, this based on ambiguous phylogenetic relationships. To test hypothesis, we analyzed phylogeny concatenated sequences (approximately 4000 bp) nuclear (18S rRNA) mitochondrial (CO1, trnV, 16S genes 18 species,...
Abstract Background Two Calyptogena clam intracellular obligate symbionts, Ca . Vesicomyosocius okutanii (Vok; C. symbiont) and Ruthia magnifica (Rma; symbiont), have small genomes (1.02 1.16 Mb, respectively) with low G+C contents (31.6% 34.0%, are thought to be in an ongoing stage of reductive genome evolution (RGE). They lack recA some genes for DNA repair, including mutY The loss is contribute the stabilization their architectures GC bias, respectively. To understand how these were lost...
The glucosinolate-myrosinase defense system (GMDS), characteristic of Brassicales, is involved in plant defense. Previous single-cell transcriptomic analyses have reported the expression profiles multiple GMDS-related cell types (i.e. myrosinase-rich myrosin idioblasts and potential glucosinolate synthetic cells as well a candidate S-cell for accumulation). However, differences stages cell-type annotation methods hindered comparisons among studies. Here, we used transcriptome extended...
Abstract It has been reported that HHV‐6 (human herpesvirus‐6) DNA identified within the female genital tract. However, clinical significance of this finding unclear. The outcome presence in tract pregnant women on their infants was evaluated present study. One hundred and ten were enrolled. Vaginal swabs collected between 4 8 weeks gestation or absence by nested polymerase chain reaction (nPCR). cultured to isolate virus. divided into two groups: DNA‐positive, negative. variables these...
Abstract Background: Congenital generalized lipodystrophy (CGL), Berardinelli‐Seip syndrome, is a rare autosomal recessive disorder characterized by the absence of adipose tissue at birth, severe insulin resistance early in life, hypertriglyceridemia, hepatomegaly, and development diabetes mellitus during puberty. Recently, two genes, BSCL2 AGPAT2 , were identified as causative genes for CGL. It has been reported that patients with BSCL mutations present more clinical findings than those...
On low intertidal and shallow subtidal shores on the west coast of Okinawa, Japan, we investigated trophic associations sacoglossan opisthobranchs associated with Bryopsidalean green algae. During 11 short research visits (55 days total) from 2002 to 2008, recorded almost 500 specimens species. These sacoglossans include a new record for Japan (Caliphylla A. Costa, 1867), recent (Placida daguilarensis Jensen, 1990), two undescribed species Trinchese, 1876 Elysia Risso, 1818), one unnamed...
Legumes develop root nodules in association with compatible rhizobia to overcome nitrogen deficiency. Rhizobia enter the host legume, mainly through infection threads, and induce nodule primordium formation cortex. Multiple transcription factors have been identified be involved regulation of establishment symbiosis, including ERF Required for Nodulation1 (ERN1). ERN1 is a network CYCLOPS NODULE INCEPTION (NIN). Mutation often results misshapen hair tips, deficient thread formation, immature...
Bradyrhizobium sp. strain SUTN9-2 is a symbiotic and endophytic diazotrophic bacterium found in legume rice plants has the potential to promote growth. The present results revealed that underwent cell enlargement, increased its DNA content, efficiently performed nitrogen fixation response extract. Some factors extract induced expression of cycle genes. According differentially expressed genes (DEGs) from transcriptomic analysis, was affected by deletion bclA gene. up-regulated DEGs encoding...
Abstract Arbuscular mycorrhizal (AM) symbiosis is a mutually beneficial interaction between fungi and land plants promotes global phosphate cycling in terrestrial ecosystems. AM are recognised as obligate symbionts that require root colonisation to complete life cycle involving the production of propagules, asexual spores. Recently it has been shown Rhizophagus irregularis can produce infection-competent secondary spores asymbiotically by adding fatty acid, palmitoleic acid. Further,...