A5029652897- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Sulfur Compounds in Biology
- Lipid metabolism and disorders
- Fibroblast Growth Factor Research
- Air Quality and Health Impacts
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- PI3K/AKT/mTOR signaling in cancer
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Alcohol Consumption and Health Effects
- Thermoregulation and physiological responses
- RNA modifications and cancer
- Cardiovascular Effects of Exercise
- Pancreatic function and diabetes
- Piperaceae Chemical and Biological Studies
- Diabetes and associated disorders
- Obstructive Sleep Apnea Research
- Cardiovascular Disease and Adiposity
- Regulation of Appetite and Obesity
- Extracellular vesicles in disease
- Calpain Protease Function and Regulation
- Dietary Effects on Health
Institut d'Investigació Biomèdica de Girona
2018-2024
Centro de Investigación Biomédica en Red
2018-2024
Instituto de Salud Carlos III
2018-2023
Institut d'Investigació Biomédica de Bellvitge
2021-2022
Clínica Girona
2020-2021
BackgroundWhile the impact of metformin in hepatocytes leads to fatty acid (FA) oxidation and decreased lipogenesis, hepatic microRNAs (miRNAs) have been associated with fat overload impaired metabolism, contributing pathogenesis non-alcoholic liver disease (NAFLD).MethodsWe investigated expression hundreds miRNAs primary challenged by compounds modulating steatosis, palmitic compound C (as inducers), an inhibitor). Then, additional hepatocyte rodent models were evaluated, together transient...
Abstract Plakophilin-2 (PKP2) is a key component of desmosomes, which, when defective, known to promote the fibro-fatty infiltration heart muscle. Less attention has been given its role in adipose tissue. We report here that levels PKP2 steadily increase during fat cell differentiation, and are compromised if adipocytes exposed pro-inflammatory milieu. Accordingly, expression subcutaneous tissue diminishes patients with obesity, normalizes upon mild-to-intense weight loss. further show...
In the present study, we aimed to investigate impact of permanent cystathionine-β-Synthase (CBS) gene knockdown in human telomerase reverse transcriptase (hTERT) immortalized adipose-derived mesenchymal stem cells (ASC52telo) and their capacity differentiate into adipocytes. CBS KD ASC52telo led increased cellular inflammation (IL6, CXCL8, TNF) oxidative stress markers (increased intracellular reactive oxygen species decreased reduced glutathione levels) parallel H2S production rejuvenation...
Aims: To investigate the impact of exogenous hydrogen sulfide (H2S) and its endogenous biosynthesis on human adipocytes adipose tissue in context obesity insulin resistance. Results: Experiments explants isolated preadipocytes demonstrated that H2S or activation resulted increased adipogenesis, action, sirtuin deacetylase, PPARγ transcriptional activity, whereas chemical inhibition gene knockdown each enzyme generating (CTH, CBS, MPST) led to altered adipocyte differentiation, cellular...
Neuregulin 4 (NRG4) has been described to improve metabolic disturbances linked obesity status in rodent models. The findings humans are controversial. We aimed investigate circulating NRG4 association with insulin action and the possible mechanisms involved. Insulin sensitivity (euglycemic hyperinsulinemic clamp) serum concentration (ELISA) were analysed subjects a wide range of adiposity (n = 89). In vitro experiments human HepG2 cell line also performed. Serum was negatively correlated (r...
Introduction Most of the disease-associated single nucleotide polymorphisms (SNPs) lie in non- coding regions human genome. Many these variants have been predicted to impact expression and function long non-coding RNAs (lncRNA), but contribution molecules development complex diseases remains be clarified. Methods Here, we performed a genetic association study between SNP located lncRNA known as LncTGM2 risk developing type 2 diabetes (T2D), analyzed its implication disease pathogenesis at...
The association among increased inflammation, disrupted iron homeostasis, and adipose tissue dysfunction in obesity has been widely recognized. However, the specific impact of inflammation on homeostasis during human adipogenesis adipocytes remains poorly understood. In this study, we investigated effects bacterial lipopolysaccharide (LPS) adipocyte differentiation, fully differentiated adipocytes, tissue. We found that LPS-induced hindered led to a gene expression profile indicative...
The H19X-encoded miR-424(322)/503 cluster regulates multiple cellular functions. Here, it is reported for the first time that also a critical linchpin of fat mass expansion. Deletion this miRNA in mice results obesity, while increasing pool early adipocyte progenitors and hypertrophied adipocytes. Complementary loss gain function experiments RNA sequencing demonstrate conserved genetic program involved differentiation commitment white Mechanistically, demonstrated targets γ-Synuclein (SNCG),...
The ribosomal protein S6 kinase 1 (S6K1) is a relevant effector downstream of the mammalian target rapamycin complex (mTORC1), best known for its role in control lipid homeostasis. Consistent with this, mice lacking S6k1 gene have defect their ability to induce commitment fat precursor cells adipogenic lineage, which contributes significant reduction mass. Here, we assess therapeutic blockage S6K1 diet-induced obese challenged LY2584702 tosylate, specific oral inhibitor initially developed...
During adipogenesis, preadipocytes' cytoskeleton reorganizes in parallel with lipid accumulation. Failure to do so may impact the ability of adipose tissue (AT) shift between storage and mobilization. Here, we identify cytoskeletal transgelin 2 (TAGLN2) as a protein expressed AT associated obesity inflammation, being normalized upon weight loss. TAGLN2 was primarily found stromovascular cell fraction, but TGF-β, estradiol also prompted increased expression human adipocytes. Tagln2 knockdown...
Lipopolysaccharide binding protein (Lbp) has been recently identified as a relevant component of innate immunity response associated to adiposity. Here, we aimed investigate the impact adipose tissue Lbp on weight gain and white (WAT) in male female mice fed an obesogenic diet. Specific gene knockdown was achieved through lentiviral particles containing shRNA-Lbp injected surgery intervention. In males, WAT mRNA levels increased parallel fat accretion, specific led reduced body gain,...
Substantial levels of lysozyme in adipose tissue association to obesity have been recently demonstrated mice and humans. In addition, experiments suggest that might impact on adipogenesis. To further investigate the relationship between adipogenesis, present study, we aimed study (Lyz2) during 3T3-L1 adipocyte differentiation its possible role Time course experiment indicated Lyz2 gene expression decreased at day 4, which was caused by isobutylmethylxanthine administration, recovered end...