A5081549402- SARS-CoV-2 and COVID-19 Research
- Endoplasmic Reticulum Stress and Disease
- COVID-19 Clinical Research Studies
- Adipose Tissue and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Long-Term Effects of COVID-19
- Ion Channels and Receptors
- Complement system in diseases
- Liver Disease Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Phagocytosis and Immune Regulation
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Protein Degradation and Inhibitors
- Neurobiology and Insect Physiology Research
- Inflammasome and immune disorders
- Immune responses and vaccinations
- Plant-based Medicinal Research
- Plant Genetic and Mutation Studies
- Marine Ecology and Invasive Species
- Invertebrate Immune Response Mechanisms
- Liver Disease and Transplantation
- Coronary Interventions and Diagnostics
Cardiff University
2021-2025
Centre for Immunity, Infection and Evolution
2025
University Hospital Ulm
2022
Hanoi Pedagogical University 2
2019
Kongju National University
2015
National Institutes of Health Clinical Center
2015
National Institutes of Health
2015
Hue University
2012-2014
Recent developments in auxin-inducible degron (AID) technology have increased its popularity for chemogenetic control of proteolysis. However, generation human AID cell lines is challenging, especially embryonic stem cells (hESCs). Here, we develop HiHo-AID2, a streamlined procedure rapid, one-step cancer and hESC with high homozygous degron-tagging efficiency based on an optimized AID2 system homology-directed repair enhancers. We demonstrate application rapid inducible functional...
Thyroid hormone responsive protein (THRSP) is a lipogenic nuclear that highly expressed in murine adipose tissue, but its role humans remains unknown.We characterized the insulin regulation of THRSP vivo human tissue biopsies and vitro Simpson-Golabi-Behmel syndrome (SGBS) adipocytes. To this end, we measured whole-body sensitivity using euglycemic clamp technique 36 subjects [age 40 ± 9 years, body mass index (BMI) 27.3 5.0 kg/m2]. Adipose were obtained at baseline after 180 360 min...
GOLM1, a Golgi membrane protein, is upregulated in cancers and liver diseases. Analysis of public RNAseq data from healthy human suggested that GOLM1 predominantly expressed cholangiocytes. Therefore, this study was initiated to understand the molecular functions cholangiocytes through protein interactomics. The findings reveal number putative GOLM1-interacting partners involved cellular regimes such as mitochondrial functions, ribonucleoprotein biogenesis, cell cycle, basement organization....
Complement is a key contributor to neuroinflammation, driving pathology in neurodegenerative diseases (NDDs); however, little known about the source of complement brain. Effective targeting NDDs requires understanding its - particular, which brain cells express genes, how expression regulated, and changes disease. To address this knowledge gap, we identified integrated single-nucleus RNA sequencing (snRNAseq) datasets from 398,097 nuclei across 48 hippocampal samples non-demented brains...
Cytopathology, crucial in disease diagnosis, commonly uses microscopic slides to scrutinize cellular abnormalities. However, processing high volumes of samples often results numerous negative diagnoses, consuming significant time and resources healthcare. To address this challenge, a surface acoustic wave-enhanced multi-view acoustofluidic rotation cytometry (MARC) technique is developed for pre-cytopathological screening. MARC enhances morphology analysis through comprehensive multi-angle...
Golgi membrane protein 1 (GOLM1) is a Golgi-resident type 2 transmembrane known to be overexpressed in several cancers, including hepatocellular carcinoma (HCC), as well viral infections. However, the role of GOLM1 lipid metabolism remains enigmatic. In this study, we employed siRNA-mediated depletion Huh-7 HCC cells study metabolism. Mass spectrometric lipidomic analysis knockdown showed an aberrant accumulation sphingolipids, such ceramides, hexosylceramides, dihexosylceramides,...
Abstract Stromal interaction molecule 1 (STIM1) and the ORAI1 calcium channel mediate store-operated entry (SOCE) regulate a multitude of cellular functions. The identity function these proteins in thyroid cancer remain elusive. We show that STIM1 expression is elevated cell lines, compared to primary cells. Knock-down or attenuated SOCE, reduced invasion, promigratory sphingosine 1-phosphate vascular endothelial growth factor-2 receptors ML-1 Cell proliferation was knock-down cells due...
The biological effects of low-dose ionizing radiation (LDIR) exposure in humans are not comprehensively understood, generating a high degree controversy published literature. earliest stages human development known to be among the most sensitive stress exposures, especially genotoxic stresses. However, risks stemming from LDIR, particularly within clinical diagnostic relevant dose range, have been directly evaluated embryonic stem cells (hESCs). Here, we describe dynamics whole genome...
The ribosomal protein S6 kinase 1 (S6K1) is a relevant effector downstream of the mammalian target rapamycin complex (mTORC1), best known for its role in control lipid homeostasis. Consistent with this, mice lacking S6k1 gene have defect their ability to induce commitment fat precursor cells adipogenic lineage, which contributes significant reduction mass. Here, we assess therapeutic blockage S6K1 diet-induced obese challenged LY2584702 tosylate, specific oral inhibitor initially developed...
Ionizing radiation (IR) is a known mutagen that widely employed for medical diagnostic and therapeutic purposes. To study the extent of genetic variations in DNA caused by IR, we used IR-sensitive human embryonic stem cells (hESCs). Four hESC cell lines, H1, H7, H9, H14, were subjected to IR at 0.2 or 1 Gy dose then maintained culture four days before being harvested isolation. Irradiation with resulted significant death, ranging from 60% 90% reduction population. Since often implicated as...
The thyroid hormone receptor beta 1 (TRβ1) is downregulated in several human cancer cell types, which has been associated with development of an aggressive tumor phenotype and the upregulation Runt-related transcription factor 2 (Runx2). In this study, we show that expression TRβ1 protein tissues lines compared normal primary line, whilst Runx2 upregulated under same conditions. contrast, upregulated, whereas downregulated, STIM1, Orai1 TRPC1 knockdown cells, to mock transfected cells. To...
본 논문에서는 TCP와 병목 링크(bottleneck link)를 공유하는 MPTCP(Multipath TCP)의 성능을 실험을 통해서 측정하고 결과를 제시하였다. MPTCP의 공정성(fairness)과 성능은 지연, 패킷 손실 등과 같은 전체 네트워크의 조건에 크게 영향을 받는다는 것을 알 수 있었다. 이상적인 일반적인 네트워크 조건에서 MPTCP는 TCP보다 상대적으로 성능이 높아서 불공정(unfair) 하지만, 지연이 증가하고 손실이 증가함에 따라 처리율(throughput)이 감소하며 TCP보다도 처리율이 감소하는 In this paper, we present experimental results evaluating the performance of Multipath TCP over shared bottleneck path in series benchmark tests. summary, find that TCP's fairness as well its...
The complement system mediates diverse regulatory immunological functions. C5aR2, an enigmatic receptor for anaphylatoxin C5a, has been shown to modulate PRR-dependent pro-inflammatory cytokine secretion in human macrophages. However, the specific downstream targets and underlying molecular mechanisms are less clear. In this study, CRISPR-Cas9 was used generate macrophage models lacking which were probe role of C5aR2 context PRR stimulation. cGAS STING-induced IFN-β significantly increased...
Today, there are different interventional approaches for patients undergoing ST elevation myocardial infarction (STEMI) with multiple vessel diseases. Objectives: to compare the mid-term results of two strategies revascularization used management STEMI Material and methods: we analyzed retrogradely 64 profiles diagnosed diseases on coronary angiography underwent angioplasty in Cardiovascular department from 5/2013 – 1/2014. The had been divided into 2 groups: group 1 (percutaneous...
Background: Cardiac resynchronization therapy is one of new methods used to treating patients with heart failure who have ventricular dyssynchrony. Objectives: 1. To study clinical, laboratory characteristis 2. the efficacy cardiac in such group patients. Materials and method: From February 2009 2011, we implanted biventricular pacemakers at Hue University Hospital for 15 had NYHA class III IV been treated optimal medical therapy, LVEF ≤35%, QRS duration ≥ 120ms. Result: 14/15 cases...