A5022216800- Microfluidic and Bio-sensing Technologies
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- 3D Printing in Biomedical Research
- Supramolecular Self-Assembly in Materials
- Origins and Evolution of Life
- Molecular Communication and Nanonetworks
- Terahertz technology and applications
- Microfluidic and Capillary Electrophoresis Applications
- Advanced biosensing and bioanalysis techniques
- Nanopore and Nanochannel Transport Studies
- Pickering emulsions and particle stabilization
- Gold and Silver Nanoparticles Synthesis and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Modular Robots and Swarm Intelligence
- Bone Tissue Engineering Materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Micro and Nano Robotics
- Luminescence and Fluorescent Materials
- Proteins in Food Systems
- Mechanical and Optical Resonators
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Nanocluster Synthesis and Applications
- Photonic and Optical Devices
Zhejiang University
2021-2025
Women's Hospital, School of Medicine, Zhejiang University
2021-2025
Medical Technologies (Czechia)
2025
University of Konstanz
2024
University of Bristol
2005-2021
École Polytechnique Fédérale de Lausanne
2012-2018
At Bristol
2017
Board of the Swiss Federal Institutes of Technology
2013
ETH Zurich
2012-2013
Nanjing University
2006-2009
Abstract The spontaneous assembly of chemically encoded, molecularly crowded, water-rich micro-droplets into periodic defect-free two-dimensional arrays is achieved in aqueous media by a combination an acoustic standing wave pressure field and situ complex coacervation. Acoustically mediated coalescence primary droplets generates single-droplet per node micro-arrays that exhibit variable surface-attachment properties, spontaneously uptake dyes, enzymes particles, display spatial...
Abstract Coacervate microdroplets produced by liquid–liquid phase separation have been used as synthetic protocells that mimic the dynamical organization of membrane‐free organelles in living systems. Achieving spatiotemporal control over droplet condensation and disassembly remains challenging. Herein, we describe formation photoswitchable behavior light‐responsive coacervate droplets prepared from mixtures double‐stranded DNA an azobenzene cation. The disassemble reassemble under UV blue...
An aqueous‐based microcompartmentalized model involving the integration of partially hydrophobic Fe(III)‐rich montmorillonite (FeM) clay particles as structural and catalytic building blocks for colloidosome membrane assembly, self‐directed remodeling, signal‐induced protocell communication is described. The colloidosomes exhibit size‐ charge‐selective permeability, show dual functions spatially confined enzyme‐mediated dephosphorylation peroxidase‐like activity. latter used...
Arrays of giant unilamellar vesicles (GUVs) with controllable geometries and occupancies are prepared by acoustic trapping used to implement chemical signaling in protocell colonies protocell/living cell consortia.
Abstract The design and assembly of artificial protocell consortia displaying dynamical behaviours systems-based properties are emerging challenges in bottom-up synthetic biology. Cellular processes such as morphogenesis differentiation rely part on reaction-diffusion gradients, the ability to mimic rudimentary aspects these non-equilibrium communities cells could provide a step life-like systems capable complex spatiotemporal transformations. Here we expose acoustically formed arrays...
Abstract The development of programmable microscale materials with cell-like functions, dynamics and collective behaviour is an important milestone in systems chemistry, soft matter bioengineering synthetic protobiology. Here, polymer/nucleotide coacervate micro-droplets are reconfigured into membrane-bounded polyoxometalate vesicles (PCVs) the presence a bio-inspired Ru-based catalyst to produce synzyme protocells (Ru 4 PCVs) catalase-like activity. We exploit for implementation...
Abstract Molecularly crowded coacervate micro‐droplets are useful protocell constructs but the absence of a physical membrane limits their application as cytomimetic models. Auxiliary surface‐active agents have been harnessed to stabilize droplets by irreversible shell formation endogenous processes reversible membranization received minimal attention. Herein, we describe dynamic alginate/silk coacervate‐based model in which membrane‐less reversibly reconfigured and inflated into...
Encapsulation of structurally and functionally intact chloroplasts within coacervate micro-droplets is used to prepare photosynthetically active membrane-free protocells.
Immobilization of compartmentalized microscale objects in 3D hydrogels provides a step towards the modular assembly soft functional materials with tunable architectures and distributed functionalities. Herein, we report use combination micro-compartmentalization, immobilization, modularization to fabricate assemble hydrogel-based microreactor assemblies comprising millions functionalized polysaccharide-polynucleotide coacervate droplets. The heterogeneous can be structurally fused by...
An ultrasound-based platform is established to prepare homogenous arrays of giant unilamellar vesicles (GUVs) or red blood cell (RBCs), hybrid assemblies GUV/RBCs. Due different responses the modulation acoustic standing wave pressure field between GUVs and RBCs, various types protocell/natural are prepared with ability undergo reversible dynamic reconfigurations from vertical horizontal alignments, 1D 2D arrangements. A two-step enzymatic cascade reaction transmitter glucose...
Acoustically trapped periodic arrays of horseradish peroxidase (HRP)-loaded poly(diallydimethylammonium chloride) / adenosine 5'-triphosphate coacervate microdroplet-based protocells exhibit a spatiotemporal biochemical response when exposed to codiffusing mixture substrate molecules (o-phenylenediamine (o-PD) and hydrogen peroxide (H2O2)) under nonequilibrium conditions. Unidirectional propagation the chemical concentration gradients gives rise time- position-dependent fluorescence signal...
Abstract Coacervate microdroplets produced by liquid–liquid phase separation have been used as synthetic protocells that mimic the dynamical organization of membrane‐free organelles in living systems. Achieving spatiotemporal control over droplet condensation and disassembly remains challenging. Herein, we describe formation photoswitchable behavior light‐responsive coacervate droplets prepared from mixtures double‐stranded DNA an azobenzene cation. The disassemble reassemble under UV blue...
Complex coacervate microdroplets, which are formed via spontaneous liquid-liquid phase separation by mixing two oppositely charged polyelectrolytes in water, have emerged as a new paradigm the fields of origin life, membraneless subcellular compartmentalization, bioreactors, and drug delivery. However, how to further improve its stability enhance selectivity one particular system remains challenge. By generating membrane-like layer at surface microdroplets electrostatic interactions between...
In order to produce materials with tailored structures and functions via supramolecular self-assembly of molecular precursors in a predictable fashion, it is necessary develop 'supramolecular methods' based on structurally simple synthons'. Thus, the formation one-dimensional aggregates from π-conjugated molecules requires combination non-covalent interactions that efficiently suppresses lateral aggregation, promotes also compatible productive π–π overlap constituent molecules. present work,...
Acoustic standing waves offer an excellent opportunity to trap and spatially manipulate colloidal objects. This noncontact technique is used for the in situ formation patterning aqueous solution of 1D or 2D arrays pH-responsive coacervate microdroplets comprising poly(diallyldimethylammonium) chloride dipeptide N-fluorenyl-9-methoxy-carbonyl-D-alanine-D-alanine. Decreasing pH preformed droplet results nanofilament self-assembly subsequent a micropatterned supramolecular hydrogel that can be...
We use an ultrasonic standing wave to simultaneously trap and deform thousands of soft lipid vesicles immersed in a liquid solution. In our device, acoustic radiation stresses comparable magnitude those generated optical stretching devices are achieved over spatial extent more than ten wavelengths. solve the scattering problem long-wavelength limit obtain stress. The result is then combined with thin-shell elasticity theory form expressions that relate deformed geometry applied field...
A biocompatible, water-soluble, lower molecular weight chitosan has been used for, via a one-pot high-temperature refluxing strategy, the simultaneous synthesis and assembly of gold nanoparticles. Transmission electron microscopy (TEM) ultraviolet−visible−near-infrared (UV−vis−NIR) extinction spectra allowed us to identify existence nanoparticle linear aggregates. Importantly, through such preparation procedure, chain length aggregates could be tuned by facile adjustment reaction reagent...
Design and construction of artificial photoresponsive protocells based on the encapsulation activation metallized peptide/porphyrin self-assembled nanofilaments within silica-nanoparticle-stabilized colloidosomes.
In recent years, surface acoustic wave (SAW) devices have demonstrated great potentials and increasing applications in the manipulation of nano- micro-particles including biological cells with advantages label-free, high sensitivity accuracy. this letter, we introduce a novel tilted-angle SAW to optimise pressure inside microchannel for cancer-cell manipulation. The generation radiation force are improved by seamlessly patterning electrodes space surrounding microchannel. Comparisons between...
Abstract Constructing a synthetic community system helps scientist understand the complex interactions among species in and its environment. Herein, two-species is constructed with A (artificial cells encapsulating pH-responsive molecules sucrose) B ( Saccharomyces cerevisiae ), which causes environment to exhibit pH oscillation behaviour due generation dissipation of CO 2 . In addition, three-species A′ containing sucrose G6P), B, C NAD + G6PDH). The solution regulates periodical release...