A5102932292- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related gene regulation
- Micro and Nano Robotics
- Photosynthetic Processes and Mechanisms
- Retinopathy of Prematurity Studies
- Signaling Pathways in Disease
- Osteoarthritis Treatment and Mechanisms
- Electrochemical sensors and biosensors
- Porphyrin and Phthalocyanine Chemistry
- Inertial Sensor and Navigation
- Ocular Surface and Contact Lens
- Molecular Communication and Nanonetworks
- Aquaculture Nutrition and Growth
- Electrocatalysts for Energy Conversion
- Aquaculture disease management and microbiota
- Cancer, Hypoxia, and Metabolism
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Allergic Rhinitis and Sensitization
- Advanced biosensing and bioanalysis techniques
- Chemokine receptors and signaling
- Origins and Evolution of Life
- Barrier Structure and Function Studies
Hunan Normal University
2021-2025
Harbin Institute of Technology
2021-2025
Sun Yat-sen University
2014-2024
Hebei Normal University
2024
China Medical University
2021
Hebei Medical University
2021
The Second Affiliated Hospital of Xiamen Medical College
2021
Shanghai Jiao Tong University
2015
Tianjin Medical University Eye Hospital
2014
Tianjin Medical University
2014
DNA-based methods for human identification principally rely upon genotyping of short tandem repeat (STR) loci. Electrophoretic-based techniques variable-length classification STRs are universally utilized, but limited in that they have relatively low throughput and do not yield nucleotide sequence information. High-throughput sequencing technology may provide a more powerful instrument identification, is currently validated forensic casework. Here, we present systematic method to perform...
Abstract Bottom-up synthesis of prototissues helps us to understand the internal cellular communications in natural tissues and their functions, as well improve or repair damaged tissues. The existed are rarely used function living We demonstrate a methodology produce spatially programmable based on magneto-Archimedes effect high-throughput manner. More than 2000 produced once within 2 h. Two-component three-component spatial coded fabricated by varying addition giant unilamellar vesicles...
The use of artificial cells to mimic living tissues is beneficial for understanding the mechanism interaction among cells. Artificial hold immense potential in field tissue engineering. Self-powered capable reversible deformation are developed by encapsulating mitochondria, actins, and methylcellulose. Upon addition pyruvate molecules, mitochondria produce adenosine triphosphate (ATP), which acts as an energy source trigger actin polymerization. occurs with a spindle shape resulting from...
Abstract Background Age-related macular degeneration (AMD), characterized by the of retinal pigment epithelium (RPE) and photoreceptors, is leading cause irreversible vision impairment among elderly. RPE senescence an important contributor to AMD has become a potential target for therapy. HTRA1 one most significant susceptibility genes in AMD, however, correlation between hasn’t been investigated pathogenesis AMD. Methods Western blotting immunohistochemistry were used detect expression WT...
The bottom-up construction of artificial cells is beneficial for understanding cell working mechanisms. glycolysis metabolism mimicry inside challenging. Herein, the glycolytic pathway (Entner-Doudoroff in archaea) reconstituted cells. comprising glucose dehydrogenase (GDH), gluconate dehydratase (GAD), and 2-keto-3-deoxygluconate aldolase (KDGA) converts molecules to pyruvate molecules. Inside cells, are further converted into alanine with help (AlaDH) build a metabolic synthesizing amino...
Abstract The construction of living systems from the bottom‐up helps to explore complex processes life and understand their working mechanism. Prototissues, constructed using artificial cells as building blocks, mimic at a high‐order tissue level, whilst usually individual cell level. 3D biomimetic prototissues demonstrate exceptional performances collective functions, which reveal mechanisms tissues hold promising potential for biomedical applications. This review systematically summarized...
Abstract Constructing a synthetic community system helps scientist understand the complex interactions among species in and its environment. Herein, two-species is constructed with A (artificial cells encapsulating pH-responsive molecules sucrose) B ( Saccharomyces cerevisiae ), which causes environment to exhibit pH oscillation behaviour due generation dissipation of CO 2 . In addition, three-species A′ containing sucrose G6P), B, C NAD + G6PDH). The solution regulates periodical release...
Purpose: The aim of this study was to elucidate the role Sema4D in pathogenesis senescence-associated choroidal neovascularization (CNV) and explore its underlying mechanisms. Methods: In study, we utilized a model laser-induced CNV both young (3 months old) old (18 mice, including those with or without knockout. expression localization were assessed using PCR, Western blot, immunostaining. Subsequently, morphological imaging examinations used evaluate size vascular leakage. Finally, M2...
Building an artificial photosynthetic cell from scratch helps to understand the working mechanisms of chloroplasts. It is a challenge achieve carbon fixation triggered by organelles in cell. ATP synthase and photosystem II (PSII) are purified reconstituted onto phospholipid membrane fabricate organelles. With integration phycocyanin, production yield increases 2.51-fold due enhanced light harvesting capability. The pathway established converting α-oxoglutarate acetyl-CoA oxaloacetate with...
Abstract Building an artificial photosynthetic cell from scratch helps to understand the working mechanisms of chloroplasts. It is a challenge achieve carbon fixation triggered by organelles in cell. ATP synthase and photosystem II (PSII) are purified reconstituted onto phospholipid membrane fabricate organelles. With integration phycocyanin, production yield increases 2.51‐fold due enhanced light harvesting capability. The pathway established converting α‐oxoglutarate acetyl‐CoA...
Purpose: KC7F2 is a novel molecule compound that can inhibit the translation of hypoxia-inducible factor 1α (HIF1α). It has been reported to exhibit potential antiangiogenic effect. We hypothesized could oxygen-induced retinal neovascularization (RNV). The purpose this study was investigate assumption. Methods: Oxygen-induced retinopathy (OIR) models in C57BL/6J mice and Sprague-Dawley rats were used for vivo study. After intraperitoneal injections KC7F2, RNV detected by immunofluorescence...
Abstract The bottom‐up constructed artificial cells help to understand the cell working mechanism and provide evolution clues for organisms. energy supply metabolism mimicry are key issues in field of cells. Herein, an containing cyanobacteria capable light harvesting carbon dioxide fixation is demonstrated produce glucose molecules by converting into chemical energy. Two downstream “metabolic” pathways starting from investigated. One involves enzyme cascade reaction H 2 O (assisted oxidase)...
Wnt/β-catenin signaling plays a key role in pathological cardiac remodeling adults. The identification of tissue-specific interaction factor may provide clinical targeting strategy. Drosophila Pygo encodes the core Wnt/β-catenin. Two homologs (Pygo1 and Pygo2) have been identified mammals. Different from ubiquitous expression profile Pygo2, Pygo1 is enriched tissue. However, mammalian disease yet to be elucidated. In this study, we found that was upregulated human tissues with hypertrophy....
Abstract Osteoarthritis (OA), a most common and highly prevalent joint disease, is closely associated with dysregulated expression modification of RXRα. However, the role RXRα in pathophysiology OA remains unknown. The present study aimed to investigate whether modulator, such as K-80003 can treat OA. Experimental was induced by intra-articular injection monosodium iodoacetate (MIA) knee rats. Articular cartilage degeneration assessed using Safranin-O fast green staining. Synovial...
Abnormal activation of the classical Wnt pathway has been reported in non-small-cell lung cancer (NSCLC) previously. Pygo family genes, core regulators Wnt/β-catenin signaling, were also to be involved tumorigenesis. However, role homolog Pygo1 human remains unclear. In current study, we demonstrated an association increased expression with consistent high nuclear β-catenin signals across pathological tissue samples early-stage NSCLC. Overexpression cells resulted enhanced G1/S cell phase...
The present study aimed to identify whether FK506 suppresses hypoxia‑induced inflammation and protects tight junction function via the calcineurin‑nuclear factor of activated T‑cells 1 (CaN‑NFATc1) signaling pathway in mouse retinal microvascular endothelial cells (mRMECs). mRMECs were treated with at different concentrations following induction hypoxia. Trans‑epithelial electrical resistance (TEER) cell permeability examined measure integrity junctions. inflammatory cytokines measured using...
To evaluate the effect of anti-allergic therapy on quality life (QoL) in children with allergic conjunctivitis (AC) and their parents.Prospective single-arm intervention study including 55 AC child-parent pairs. The endpoint was that successfully controlled after therapies. primary outcome change QoL from baseline to as measured by total Pediatric Quality Life Inventory (PesdQL) score.The PedsQL scores parents were improved treatment (P < .001). greater vernal keratoconjunctivitis/atopic...