A5076642717- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Cardiovascular Effects of Exercise
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Congenital heart defects research
- Cardiomyopathy and Myosin Studies
- Peroxisome Proliferator-Activated Receptors
- Inflammasome and immune disorders
- Neurobiology and Insect Physiology Research
- Cardiovascular Function and Risk Factors
- Gout, Hyperuricemia, Uric Acid
- Cellular Mechanics and Interactions
- Telomeres, Telomerase, and Senescence
- MicroRNA in disease regulation
- Coronary Artery Anomalies
- Mesenchymal stem cell research
- Developmental Biology and Gene Regulation
- Anesthesia and Neurotoxicity Research
- Spinal Cord Injury Research
- Hydrogen's biological and therapeutic effects
- Medicinal Plant Pharmacodynamics Research
- Hippo pathway signaling and YAP/TAZ
- Congenital Heart Disease Studies
Hunan Normal University
2014-2025
Changsha Normal University
2022-2023
First People's Hospital of Yunnan Province
2020
Non-alcoholic fatty liver disease (NAFLD) is a common that causes serious damage. Exercise recognized as non-pharmacological tool to improve the pathology of NAFLD. However, antioxidative effects and mechanisms by which exercise ameliorates NAFLD remain unclear. The present study conducted training on zebrafish during 12-week high-fat feeding period antioxidant effect liver. We found swimming decreased lipid accumulation improved pathological changes in diet-fed zebrafish. Moreover,...
Sarcopenia is a common skeletal muscle degenerative disease characterized by decreased mass and mitochondrial dysfunction that involves microRNAs (miR) as regulatory factors in various pathways. Exercise reduces age-related oxidative damage chronic inflammation increases autophagy, among others. Moreover, whether aerobic exercise can regulate homeostasis modulating the miR-128/insulin-like growth factor-1 (IGF-1) signaling pathway improve sarcopenia requires further investigation....
Congenital heart defects (CHDs) are common human birth defects. Genetic mutations potentially cause the exhibition of various pathological phenotypes associated with CHDs, occurring alone or as part certain syndromes. Zebrafish, a model organism strong molecular conservation similar to humans, is commonly used in studies on cardiovascular diseases owing its advantageous features, such similarity electrophysiology, transparent embryos and larvae for observation, suitability forward reverse...
Obesity is a highly prevalent disease that can induce metabolic syndrome and associated with greater risk of muscular atrophy. Mitochondria play central roles in regulating the physiological metabolism skeletal muscle; however, whether decreased mitochondrial function impaired muscle unclear. In this study, we evaluated effects high-fat diet on zebrafish model sarcopenic obesity (SOB). SOB zebrafish, significant decrease exercise capacity fiber cross-sectional area was detected, accompanied...
Introduction Recent reports indicate that mitochondrial quality decreases during non-alcoholic fatty liver disease (NAFLD) progression, and targeting the mitochondria may be a possible treatment for NAFLD. Exercise can effectively slow NAFLD progression or treat However, effect of exercise on in has not yet been established. Methods In present study, we fed zebrafish high-fat diet to model NAFLD, subjected swimming exercise. Results After 12 weeks, significantly reduced diet-induced injury,...
Type 2 diabetes mellitus (T2DM) is a common metabolic disease that frequently accompanied by multiple complications, including diabetic myopathy, muscle disorder mainly manifested as decreased function and reduced mass. Diabetic myopathy relatively complication among patients with attributed to mitochondrial dysfunction. Therefore, we investigated the mechanisms underlying development, focusing on role of microRNAs (miRs). Zebrafish were fed high-sugar diet for 8 weeks immersed in glucose...
Background: Growing evidence suggests that the prevalence of sarcopenic obesity (SOB) is on rise across globe. However, key molecular mechanisms behind this disease have not been clarified. Methods: In experiment, we fed zebrafish a high-fat diet (HFD) for 16 weeks to induce obesity. Results: After dietary trial, HFD exhibited an obese phenotype with skeletal muscle atrophy and decreased swimming capacity. We demonstrated mitochondrial content function were abnormal in SOB muscle. These...
Sarcopenia is a common disorder that leads to progressive decrease in skeletal muscle function elderly people. Exercise effectively prevents or delays the onset and progression of sarcopenia. However, molecular mechanisms underlying how exercise intervention improves atrophy remain unclear. In this study, we found 21‐month‐old zebrafish had decreased swimming ability, reduced fibre cross‐sectional area, unbalanced protein synthesis, degradation, increased oxidative stress, mitochondrial...
Excessive alcohol consumption can cause alcoholic myopathy, but the molecular mechanism is still unclear. In this study, zebrafish were exposed to 0.5% for eight weeks investigate effect of on skeletal muscle and its mechanism. The results showed that body length, weight, cross-sectional area fibers, Ucrit, MO2max significantly decreased after exposure. expression markers atrophy autophagy was increased, P62 reduced. content ROS, mRNA sod1 sod2, protein Nox2 increased. addition, we found...
Magnetoreception is essential for magnetic orientation in animal migration. The molecular basis magnetoreception has recently been elucidated fruitfly as complexes between the receptor magnetoreceptor (MagR) and its ligand cryptochrome (Cry). MagR Cry are present kingdom. However, it unknown whether they perform a conserved role diverse animals. Here we report identification expression of zebrafish homologs towards understanding their roles lower vertebrates. A single magr gene 7 cry genes...
Herein, we aimed to establish an aerobic exercise-induced physiological myocardial hypertrophy zebrafish (Danio rerio) model and explore the underlying molecular mechanism. After 4 weeks of exercise, AMR Ucrit increased hearts were enlarged, with thickened myocardium, number myofilament attachment points in Z-line, compaction mitochondrial cristae. We also found that mTOR signaling pathway, angiogenesis, fusion, fission event, autophagy associated adaptive changes heart during training. In...
Background: Previously, we first identified the human tripartite motifcontaining protein 45 (TRIM45) acts as a novel transcriptional repressor in mitogenactivated kinase (MAPK) signaling pathway. After that, inhibitory role of TRIM45 development tumor was gradually unveiled. However, function tumorigenesis lung cancer has not been characterized. Methods and Results: In this study, found that up-regulated earlystage non-small-cell (NSCLC) tissues. Overexpression cells induces G1 arrest...
Long-term imbalance between fatigue and recovery may eventually lead to muscle weakness or even atrophy. We previously reported that excessive exercise induces pathological cardiac hypertrophy. However, the effect of on skeletal muscles remains unclear. In present study, we successfully established an excessive-exercise-induced atrophy zebrafish model, with decreased fiber size, critical swimming speed, maximal oxygen consumption. High-throughput RNA-seq analysis identified differentially...
Bushen Yiyuan recipe (BYR) is an effective Chinese prescription with antifatigue and antioxidation effects.The effects of BYR on testosterone synthesis in rat Leydig cells exercise-induced low serum levels (EILST) are assessed.Thirty-two Sprague-Dawley rats were chronically trained for 6 weeks to establish EILST model. divided into model (physiological saline), EFE (700 mg/kg ethanol extract Epimedii folium, the dried leaves Epimedium brevicornu Maxim [Berberidaceae]), groups (350 700 mg/kg)...
Myocardial infarction is due to lasting and serious myocardial ischemic that leads necrosis cardiac remodeling, can consequently results in heart failure. Traditional treatment for includes drug therapy, thrombolytic percutaneous coronary intervention (PCI), artery bypass graft (CABG) surgery. Although these treatments some extent relieve the symptom of ischemia, they fail repair necrotic tissues. regeneration undoubtedly best solution clinical problems treatment. The review briefly...
Exercise-induced cardiac remodeling has aroused public concern for some time, as sudden death is known to occur in athletes; however, little about the underlying mechanism of exercise-induced injury. In present study, we established an excessive pathologic hypertrophy model zebrafish with increased myocardial fibrosis, myofibril disassembly, mitochondrial degradation, upregulated expression pathological marker genes heart, contractile impairment, and cardiopulmonary function impairment....
Wnt/β-catenin signaling plays a key role in pathological cardiac remodeling adults. The identification of tissue-specific interaction factor may provide clinical targeting strategy. Drosophila Pygo encodes the core Wnt/β-catenin. Two homologs (Pygo1 and Pygo2) have been identified mammals. Different from ubiquitous expression profile Pygo2, Pygo1 is enriched tissue. However, mammalian disease yet to be elucidated. In this study, we found that was upregulated human tissues with hypertrophy....
摘要: 本课题组前期研究中, 利用斑马鱼cmlc2 (Cardiac myosin light chain 2)基因启动子构建了一个用于斑马鱼心脏组织特异表达外源基因的转基因表达载体pTol2-cmlc2-IRES-EGFP。文章利用该载体构建了一个稳定表达EGFP的转基因斑马鱼品系, 并初步分析了EGFP的表达对该转基因斑马鱼品系的心脏发育和功能的影响。结果表明, 在建立的转基因斑马鱼品系早期胚胎发育过程中, 绿色荧光信号在心脏中特异表达, 该表达模式与原位杂交分析的cmlc2的表达模式结果相同; 该转基因斑马鱼品系的心脏形态及发育生长正常; 进一步通过M-Mode分析心脏生理学功能的结果表明:该转基因品系心动周期、心率、收缩与舒张表面积及表面积缩短率等重要生理指标与正常野生型的斑马鱼对照组相比没有显著差别。以上结果表明该转基因品系中绿色荧光蛋白的表达对斑马鱼心脏的发育和功能没有影响。研究结果为进一步利用该载体建立外源目的基因转基因表达模型, 研究心脏表达基因的功能奠定了重要基础。