A5040418568- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Transgenic Plants and Applications
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Antimicrobial Resistance in Staphylococcus
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Animal Virus Infections Studies
- Microbial Metabolism and Applications
- Biochemical and Structural Characterization
- Mycobacterium research and diagnosis
- Fungal Infections and Studies
- Immunodeficiency and Autoimmune Disorders
- Animal Genetics and Reproduction
- DNA and Nucleic Acid Chemistry
- CAR-T cell therapy research
- Bioactive Compounds and Antitumor Agents
- Toxoplasma gondii Research Studies
- Biotin and Related Studies
- Skin Protection and Aging
- Trypanosoma species research and implications
Tokushima University
2014-2023
Institute of Engineering Science
2014
Nara Institute of Science and Technology
2006-2009
Johns Hopkins University
2006
Abstract Most cells active in the immune system express receptors for antibodies which mediate a variety of defensive mechanisms. These interact with Fc portion antibody and are therefore collectively called receptors. Here, using high-speed atomic force microscopy, we observe interactions human, humanized, mouse/human-chimeric immunoglobulin G1 (IgG1) their cognate receptor, FcγRIIIa. Our results demonstrate that not only but also Fab positively contributes to interaction receptor....
Do polypeptide chains ever behave like a random coil? In this report we demonstrate that glycine, the residue with fewest backbone restrictions, exhibits strong preference for an extended conformation in solution when polymerized short segments of polyglycine. A model peptide system comprised two unique tripeptide units, between which 1 to 18 glycine residues are inserted, is characterized by NMR and small-angle X-ray scattering (SAXS). The residual dipolar coupling (RDC) values units...
Abstract A considerable number of functional proteins are unstructured under physiological condition. These “intrinsically disordered” exhibit induced folding when they bind their targets. The comprises two elementary processes: and binding. Two mechanisms possible for the folding: either before binding or folding. We found that these can be distinguished by target‐concentration dependence kinetics. also created types mutants staphylococcal nuclease showing different inhibitor‐concentration...
One approach to creating more beneficial therapeutic antibodies is develop bispecific (bsAbs), particularly IgG-like formats with tetravalency, which may provide several advantages such as multivalent binding each target antigen. Although the effects of configuration and antibody-fragment type on function bsAbs have been studied, there only a few detailed studies influence variable fragment domain order. Here, we prepared four types hEx3-scDb-Fc, bsAbs, built from single-chain hEx3-Db...
Although interactions of small molecular drugs with serum proteins have been widely studied from pharmacokinetic and pharmacodynamic perspectives, there few reports on the effects components therapeutic antibody functions. This study effect abundant antibody-dependent cellular cytotoxicity (ADCC) mediated by rituximab Fcγ receptor III (FcγRIII). Human albumin (HSA) Fab fragment pooled polyclonal IgG were found to compromise ADCC as non-competitive inhibitors. Our nuclear magnetic resonance...
Ultraviolet-A (UV-A) can damage microbes by generating reactive oxygen species (ROS), singlet oxygen, superoxides, hydrogen peroxide and hydroxyl radicals. These readily react with lipids, proteins, DNA other constituents of cells, leading to oxidative deterioration the eventual death microbe. However, ability these also harms viability mammalian cells such as fibroblasts keratinocytes, they cause both acute chronic damage, photo-aging, photo-carcinogenesis. This study describes a UV-A...
Galactosialidosis (GS) is a lysosomal cathepsin A (CTSA) deficiency. It associates with simultaneous decrease of neuraminidase 1 (NEU1) activity and sialylglycan storage. Central nervous system (CNS) symptoms reduce the quality life juvenile/adult-type GS patients, but there no effective therapy. Here, we established novel model mouse carrying homozygotic Ctsa IVS6+1g→a mutation causing partial exon 6 skipping concomitant deficiency Ctsa/Neu1. The mice developed juvenile/adult GS-like...
Recombinant antibodies have been investigated and used in applications such as targeting modules of drug-delivery systems (DDS) against cancers. This study aimed to prepare recombinant HER2, containing sortase A (SrtA) recognition sequence, that are applicable DDS after linkage with the drug-carrier oligoglycine-acceptor peptide by SrtA transpeptidation.The trastuzumab fragment (scFvs Fab) SrtA-recognition motif (LPXTG) at their C-terminal were constructed expressed Escherichia coli Chinese...
Baculovirus-infected silkworms are promising bioreactors for producing recombinant glycoproteins, including antibodies. Previously, we developed a method isotope labeling of glycoproteins nuclear magnetic resonance (NMR) studies using silkworm larvae reared on an artificial diet containing 15N-labeled yeast crude protein extract. Here, further develop this by introducing technique the expression isotope-labeled pupae, which has several potential advantages relative to larvae-based techniques...
Recombinant immunoglobulin G (IgG) aggregates are formed during their production. However, the process underlying intracellular/extracellular aggregation in cell culture conditions is not well understood, and no effective method exists to assess IgG aggregates. Here, we establish an approach detect intracellular using AF.2A1, a small artificial protein that binds non-native conformers Fluorescent-labeled AF.2A1 prepared via conjugation transfected into antibody-producing Chinese hamster...
Background Therapeutic antibodies have become an important focus of the biopharmaceutical industry. The Chinese hamster ovary (CHO) cell line is a major host for therapeutic antibody production. To construct productive CHO lines, two transfection methods are commonly used, i.e., random integration and gene targeting. Random common method in which randomly integrated transgenes cause variation productivity because they located various chromosomal regions that affect transgene expression...