A5063355828- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Tactile and Sensory Interactions
- Pluripotent Stem Cells Research
- Advancements in Transdermal Drug Delivery
- Textile materials and evaluations
- Supramolecular Chemistry and Complexes
- Animal Genetics and Reproduction
- Supramolecular Self-Assembly in Materials
- Birth, Development, and Health
- Cancer-related molecular mechanisms research
- Ocular Surface and Contact Lens
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Biochemical Acid Research Studies
- Diet, Metabolism, and Disease
- Vitamin C and Antioxidants Research
- Plant Pathogenic Bacteria Studies
- RNA regulation and disease
- Coenzyme Q10 studies and effects
Kyoto University
2025
Tokai University
2023-2024
Yamagata University
2021-2024
Japanese Foundation For Cancer Research
2020-2022
California Institute for Regenerative Medicine
2020-2022
University of Pennsylvania
2020-2022
Tokyo Women's Medical University
2022
Tokyo Women's Medical University Yachiyo Medical Center
2022
Philadelphia University
2022
Kindai University
2015-2018
Abstract Plants lack specialized and mobile immune cells. Consequently, any cell type that encounters pathogens must mount responses communicate with surrounding cells for successful defence. However, the diversity, spatial organization function of cellular states in pathogen-infected plants are poorly understood 1 . Here we infect Arabidopsis thaliana leaves bacterial trigger or supress integrate time-resolved single-cell transcriptomic, epigenomic transcriptomic data to identify states. We...
Abstract Establishment of spermatogonia throughout the fetal and postnatal period is essential for production spermatozoa male fertility. Here, we establish a protocol in vitro reconstitution human prospermatogonial specification whereby primordial germ cell (PGC)-like cells differentiated from induced pluripotent stem are further into M-prospermatogonia-like T1 prospermatogonia-like (T1LCs) using long-term cultured xenogeneic reconstituted testes. Single RNA-sequencing used to delineate...
A hepatoprotective peptide, pyroglutamyl leucine (pyroGlu-Leu), was identified in wheat gluten hydrolysate through an vivo activity-guided fractionation approach based on D-galactosamine-induced acute hepatitis rats and of peptides with large-scale preparative ampholine-free isoelectric focusing. The active acidic fraction predominantly consisted free pyroglutamic acid. Pyroglutamyl were derivatized phenyl isothiocyanate after removal a residue by pyroglutamate aminopeptidase. derivatives...
Development of the adrenal cortex, a vital endocrine organ, originates in adrenogonadal primordium, common progenitor for both adrenocortical and gonadal lineages rodents. In contrast, we find that humans cynomolgus monkeys, lineage temporally spatially distinct fashion from lineage, arising earlier more anteriorly within coelomic epithelium. The primordium arises adrenogenic epithelium via an epithelial-to-mesenchymal transition, which then progresses into steroidogenic fetal zone direct...
Xist RNA, which is responsible for X inactivation, a key epigenetic player in the embryogenesis of female mammals. Of several repeats conserved A-repeat has been shown to be essential its silencing function differentiating embryonic stem cells. Here, we introduced new allele into mouse that produces mutated RNA lacking (XistCAGΔ5' ). XistCAGΔ5' expressed embryo coated chromosome but failed silence it. Although imprinted inactivation was substantially compromised upon paternal transmission,...
The nucleolus is the site of ribosome assembly and formed through liquid-liquid phase separation. Multiple ribosomal DNA (rDNA) arrays are bundled in nucleolus, but underlying mechanism significance unknown. In present study, we performed high-content screening followed by image profiling with wndchrm machine learning algorithm. We revealed that cells lacking a specific 60S protein set exhibited common nucleolar disintegration. depletion RPL5 (also known as uL18), separation facilitator, was...
X chromosome inactivation (X-inactivation) in female mammals is triggered by differential upregulation of the Xist gene on one two chromosomes and subsequent coating cis with its non-coding transcripts. Although targeted mutation has clearly shown that essential for X-inactivation cis, molecular mechanism which RNA induces silencing largely unknown. Here, we demonstrate an mutant generated previously mouse targeting, Xist(IVS), unique it partially retains capacity to silence chromosome....
Moisturization causes physiological changes that improve the barrier function of human skin and mechanical changes, including friction characteristics. This study evaluated petrolatum- or silicone oil-treated to determine effect moisturizing on dynamics. The force was measured using a contact probe with sinusoidal motion. used rub upper arm 20 subjects. water content stratum corneum, softness, were corneometer, cutometer, tewameter, respectively. Both oils reduce frictional skin....
Coenzyme Q10 (CoQ10) has been widely commercially available in Japan as a dietary and health supplement since 2001 is used for the prevention of lifestyle-related diseases induced by free radicals aging. We evaluated CoQ10 supplements to ensure that these can be effectively safely. Commercially products were selected assessed quality control tests specified Japanese Pharmacopoeia XV. When disintegration time was measured, few tested did not completely disintegrate even after incubation water...
Evaluating friction in human skin is important to assess its condition and the effects of skincare cosmetics. In this study, we evaluated dynamics moisturized show moisturization on mechanical properties.Friction force was using a sinusoidal motion evaluation system. The upper arm 20 subjects rubbed contact probe. water content stratum corneum softness were measured Corneometer Cutometer, respectively.When treated with or 10 wt% glycerol aqueous solution, coefficients increased by 0.23 ±...
Abstract The mutation of the X-linked protocadherin (PCDH) 19 gene in heterozygous females causes epilepsy. However, because erosion X-chromosome inactivation (XCI) female human pluripotent stem cells, precise disease modeling often leads to failure. In this study, using a mathematical approach and induced cells retaining XCI derived from patients with PCDH19 missense mutations, we found that heterotypic conditions, which are composed wild-type PCDH19, led significant cell-to-cell proximity...
Abstract In the nucleus, genomic DNA is wrapped around histone octamers to form nucleosomes. principle, nucleosomes are substantial barriers transcriptional activities. Nuclear non-coding RNAs (ncRNAs) proposed function in chromatin conformation modulation and regulation. However, it remains unclear how ncRNAs affect nucleosome structure. Eleanors clusters of that accumulate estrogen receptor-α ( ESR1 ) gene locus long-term deprivation (LTED) breast cancer cells, markedly enhance...
The dosage difference of X-linked genes between the sexes in mammals is compensated for by genetically inactivating one or other X chromosomes XX females. A noncoding RNA transcribed from Xist gene at onset X-inactivation coats chromosome cis and induces chromosome-wide heterochromatinization. Here, we report a new allele (XistCAG) driven CAG promoter, which known to be constitutively active many types cells. paternal transmission XistCAG resulted preferential inactivation targeted (Xp) not...
„Superfilm“: Zweidimensionale Blattstrukturen aus alkylsilylierten Desoxyguanosin-Derivaten wurden wasserstoffverbrückten eindimensionalen Bändern erhalten. Durch Einführen polarer Oxyethylen-Einheiten auf der Oberfläche konnten elastische stabile supramolekulare Filme mit definiertem hierarchischem Aufbau konstruiert werden.
High-resolution imaging analysis using various types of cells is an essential tool for dissecting cell functions. Generally, obtaining such images requires the to be cultured on glass substrates; however, it often results in unstable status cells. In this study, we report that coating substrate nanosheet composed hydrophobic polystyrene, with Matrigel, significantly improves viability human pluripotent stem (hPSCs). Moreover, does not affect transcriptome hPSC and enables researchers perform...
Herein, we evaluated friction dynamics of human skin treated with polyacrylic acid aqueous solutions or gel creams using a sinusoidal motion evaluation system to demonstrate the effect treatment polymer on skin. A solution cream was applied inner forearms 10 subjects evaluate temporal changes in force under motion. Water content, viscoelasticity, and transepidermal water loss were also simultaneously measured determine effects conditions. When solution, coefficient immediately after...
ABSTRACT Development of the adrenal cortex, a vital endocrine organ, originates in adrenogonadal primordium, common progenitor for both adrenocortical and gonadal lineages rodents. In contrast, we find that humans cynomolgus monkeys, lineage temporally spatially distinct fashion from lineage, arising earlier more anteriorly within coelomic epithelium. The primordium arises adrenogenic epithelium via an epithelial-to- mesenchymal-like transition, which then progresses into steroidogenic fetal...
ABSTRACT Establishment of spermatogonia throughout the fetal and postnatal period is essential for production spermatozoa male fertility. Here, we established a protocol in vitro reconstitution human prospermatogonial specification whereby primordial germ cell (PGC)-like cells (hPGCLCs) differentiated from induced pluripotent stem were further into M-prospermatogonia-like (MLCs) T1 prospermatogonia-like (T1LCs) using long-term cultured xenogeneic reconstituted testes. Single RNA-sequencing...
Abstract The mechanisms leading to adrenal cortex development and steroid synthesis in humans remain poorly understood due the paucity of model systems. Herein, we faithfully recapitulate human fetal specification processes through stepwise induction induced pluripotent stem cells posterior intermediate mesoderm-like progenitor-like states ultimately generate zone cortex-like (FZLCs), as evidenced by histomorphological, ultrastructural, transcriptome features adrenocorticotropic hormone...