A5001763680- Mesenchymal stem cell research
- Dental Implant Techniques and Outcomes
- Periodontal Regeneration and Treatments
- Nerve injury and regeneration
- Tendon Structure and Treatment
- Orthodontics and Dentofacial Orthopedics
- Bone fractures and treatments
- Dental Radiography and Imaging
- Muscle Physiology and Disorders
- Metal and Thin Film Mechanics
- Bone Tissue Engineering Materials
- Developmental Biology and Gene Regulation
- Proteoglycans and glycosaminoglycans research
- Cancer-related gene regulation
- Semiconductor materials and devices
- dental development and anomalies
- Ultrasound and Hyperthermia Applications
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- Bone and Dental Protein Studies
- Salivary Gland Disorders and Functions
- Endodontics and Root Canal Treatments
- ZnO doping and properties
- Salivary Gland Tumors Diagnosis and Treatment
- Autonomous Vehicle Technology and Safety
Sichuan University
2012-2025
West China Hospital of Sichuan University
2014-2025
State Key Laboratory of Oral Diseases
2012-2025
Shandong University
2022-2023
Southeast University
2020
Shanghai Institute of Applied Physics
2018
Chinese Academy of Sciences
2018
Changzhou University
2013-2016
Abstract A reciprocal relationships between osteogenesis and adipogenesis has been observed in vitro vivo , mechanical stretch believed to be a regulating factor of osteo‐adipogenic axis differentiation mesenchymal stem cells. In this study, rat adipose cells (ASCs) were isolated cultured adipogenic or normal medium. Their exposure cyclic (2000 με, 1 Hz) the presence medium decreased mRNA protein level PPAR‐γ, increased Runx2 levels as well Pref‐1 level, compared static samples. ASCs without...
Abstract Objective: To determine the inhibitory effect and mechanism of Notch signalling on adipogenesis mouse adipose‐derived stem cells (mASCs). Materials methods: Varied concentrations N ‐[ ‐(3,5‐difluorophenacetyl)‐ l ‐alanyl]‐ S ‐phenylglycine t‐butylester (DAPT) were added to mASCs 3 days before adipogenic induction with insulin‐containing differentiation medium. The process ability lipid droplet accumulation analysed using oil red‐O staining. pathway (Notch‐1, ‐2, ‐3, ‐4, Hes‐1 Hey‐1)...
Mechanical forces play critical roles in the development and remodelling process of bone. As an alternative cell source for bone engineering, adipose-derived stem cells (ASCs) should be fully investigated their responses to mechanical stress mechanisms responsible osteogenic induction response signals.We hypothesized that appropriate application uniaxial cyclic tensile strain ASCs could increase morphogenetic protein-2 (BMP-2) expression improve osteogenesis ASCs. To test our hypothesis,...
A 1-2 mm thick labial plate is generally advocated in immediate implant placement and provisionalization (IIPP). However, most of the human plates fail to meet this requirement.This study aimed investigate effect thickness on hard tissue, soft esthetic outcomes IIPP.In prospective cohort study, 40 patients received IIPP 50 single-crown implants anterior maxilla. Patients were categorized into three groups according their presurgical bone: 0-0.5, 0.5-1, ≥ 1 mm. CBCT, mucosa recession papilla...
Cartilage maintains the structure and function of joints, with disturbances leading to potential osteoarthritis. N6-methyladenosine (m6A), most widespread post-transcriptional modification in eukaryotes, plays a crucial role regulating biological processes. While current research has indicated that m6A affects progression osteoarthritis, its development homeostasis articular cartilage remains unclear. Here we report Mettl3 deficiency chondrocytes leads mandibular condylar morphological...
Mesenchymal stem cells (MSCs) provide us an excellent cellular model to uncover the molecular mechanisms underlying adipogenic differentiation of adult cells. PPARgamma had been considered as important marker undergoing differentiation. Here, we demonstrated that expression and phosphorylation could be found in bone marrow-derived MSCs cultured expansion medium without any additives (dexamethasone, IBMX, insulin or indomethacin). Then, was dephosphorylated during process We then inhibition...
Abstract Objectives This study aimed to investigate the response of Schwann cells cyclic compressive and tensile stresses different durations stimulation. Materials methods RSC96 were subjected stress or stress; for either, in five groups treated 0, 1, 2, 24 48 h respectively. Enzyme‐linked immunosorbent assay was conducted detect secretion nerve growth factor ( NGF ), brain‐derived neurotrophic BDNF neurotrophin‐3 neurotrophin‐4 culture medium. Real‐time PCR quantify mRNA expression...
Abstract Objectives Peripheral nerve injuries are a common occurrence, resulting in considerable patient suffering; it also represents major economic burden on society. To improve treatment options following peripheral injuries, scientists aim to find way promote Schwann cell ( SC ) myelination help nerves carry out their functions effectively. In this study, we investigated ability of s, regulated by co‐culture with adipose‐derived stem cells ASC s) or low‐intensity pulsed ultrasound LIPUS...
ABSTRACT Objective This study aimed to compare the clinical effects of implants placed in sites with a transcrestal sinus floor elevation (TSFE) featuring residual bone height (RBH) ≤ 5 mm, without Schneiderian membrane perforations, those where lateral window approach was utilized repair perforations that occurred during TSFE. Methods A total 104 80 patients (80 sinuses) RBH mm who had undergone TSFE simultaneous implant placement were included this retrospective study. The categorized into...
ABSTRACT Objectives To investigate the clinically acceptable deviation threshold and factors associated with trueness across three computer‐assisted implant surgery (CAIS) approaches: static CAIS (s‐CAIS), dynamic (d‐CAIS), robotic (r‐CAIS). Materials Methods A total of 314 implants in 194 patients were retrospectively included, preoperative postoperative CBCT scans collected. Outcome measures included platform, apex, angular deviations, which assessed by registering to plans using a jawbone...
Abstract Enzymatic digestion, the commonly used method of adipose‐derived stromal cells isolation, is time consuming and expensive, especially when applied to large volumes tissue. In present study, characteristics obtained by adipose tissue explant culture were studied. We found that fragments could adhere onto growth surface flasks in a very short after plating fibroblast‐like migrated from explants reached confluence. Morphologic analysis markers expression suggested mesenchymal origin...
As the most important organs of occlusion, teeth are subjected to a variety mechanical stresses. These stresses transmitted into dental pulp tissue and affect stem cells. In this study, human cells were isolated from impacted third molars their multilineage differentiation abilities tested. Human then exposed cyclic tensile stretch. The results showed that stretch inhibited expression osteogenic marker genes proteins such as BMP-2, OCN ALP. Simultaneously, odontogenic DSPP, DSP BSP also by...
Abstract Objective The aim of this study was to investigate effects mechanical compressive force on differentiation adipose‐derived stem cells ( ASC s) in vitro . Materials and methods Mice s were treated with (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction 3 days, then oil red O staining used examine droplet‐filled cells. Adipogenic genes, PPAR ‐γ1 APN , examined by real‐time PCR immunofluorescence IF ) performed test expression de‐ ‐γ ph‐ at the protein level. Results Our data...
To investigate the effect of DAPT (γ‐secretase inhibitor) on growth human tongue carcinoma cells and to determine molecular mechanism enable potential application treatment carcinoma. Human Tca8113 were cultured with DAPT. Cell was determined using Indigotic Reduction method. The cell cycle apoptosis analyzed by flow cytometry. Real‐time PCR Immuno‐Fluorescence (IF) employed intracellular expression levels. inhibited inducing G0–G1 arrest apoptosis. mRNA levels Hairy/Enhancer Split‐1...
Abstract Objective The aim of this study was to investigate effects low‐intensity pulsed ultrasound ( LIPUS ) on differentiation adipose‐derived stem cells ASC s), in vitro . Materials and methods Murine s were treated with for either three or five days, immediately after adipogenic induction, delayed 2 days. Expression genes PPAR ‐γ1 , APN examined by real‐time PCR Immunofluorescence IF staining performed test PPAR‐γ at the protein level. Results Our data revealed that specific patterns...
The purpose of this study was to evaluate the effect different implant surface properties on morphology, proliferation, neurotrophin secretion, and gene expression Schwann cells. Four types surfaces, including ground (smooth surface), sandblasted acid-etched (SLA), hydroxyapatite-coated (HA), titanium plasma spray (TPS) surfaces were fabricated photographed by a scanning electron microscopy (SEM). cells derived from neonatal rats cultured assessed via SEM observation methylthiazol...
The present study aimed to elucidate whether extracellular signal-regulated kinases 1/2 (ERK1/2) and p38 mitogen-activated protein pathways participate in the transduction of mechanical stretch exerted on adipose stem cells (ASCs) into intracellular osteogenic signals, if so both have time-dependent feature. Rat ASCs were cultured medium for 72 h assigned three sets, namely ERK1/2 inhibitor treated set, control set. After treatment, all subjected cyclic stretch(2000 με, 1 Hz) a four-point...