A5041837833- Neuroinflammation and Neurodegeneration Mechanisms
- Traumatic Brain Injury and Neurovascular Disturbances
- Intracerebral and Subarachnoid Hemorrhage Research
- Neonatal and fetal brain pathology
- Neurogenesis and neuroplasticity mechanisms
- Acute Ischemic Stroke Management
- Immune cells in cancer
- Atherosclerosis and Cardiovascular Diseases
- Neurological Disorders and Treatments
- Neuroscience and Neuropharmacology Research
- Neurological Disease Mechanisms and Treatments
- Team Dynamics and Performance
- Psychological and Educational Research Studies
- Sperm and Testicular Function
- Phagocytosis and Immune Regulation
- Death Anxiety and Social Exclusion
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CAR-T cell therapy research
- Immune Response and Inflammation
- Retinal Development and Disorders
- MicroRNA in disease regulation
- IL-33, ST2, and ILC Pathways
- Animal Genetics and Reproduction
- Traumatic Brain Injury Research
- Vagus Nerve Stimulation Research
University of Pittsburgh
2018-2023
Geriatric Research Education and Clinical Center
2022
Westchester Medical Center
2021
New York Medical College
2021
University of Pittsburgh Medical Center
2019
Iowa State University
1998
University of Bern
1998
Intracerebral hemorrhage (ICH) is a devastating form of stroke affecting millions people worldwide. Parenchymal hematoma triggers series reactions leading to primary and secondary brain injuries permanent neurological deficits. Microglia macrophages carry out clearance, thereby facilitating functional recovery after ICH. Here, we elucidate pivotal role for the interleukin (IL)-4)/signal transducer activator transcription 6 (STAT6) axis in promoting long-term both blood- collagenase-injection...
The repair of white matter damage is paramount importance for functional recovery after brain injuries. Here, we report that interleukin-4 (IL-4) promotes oligodendrocyte regeneration and remyelination. IL-4 receptor expression was detected in a variety glial cells ischemic injury, including lineage cells. deficiency knockout mice resulted greater deterioration over 14 d stroke. Consistent with these findings, intranasal delivery nanoparticles stroke improved integrity attenuated long-term...
Regulatory T cells (Tregs) are known to protect against ischemic stroke. However, the low frequency of Tregs restricts their clinical utility. This study investigated whether expanding number in vivo with IL-2/IL-2 antibody complex (IL-2/IL-2Ab) could improve stroke outcomes and further elaborated mechanisms protection male mice. C57BL/6 mice received IL-2/IL-2Ab or isotype IgG (IsoAb) intraperitoneally for 3 d before (pretreatment) starting 2 h after (posttreatment) 60 min middle cerebral...
Immunomodulation holds therapeutic promise against brain injuries, but leveraging this approach requires a precise understanding of mechanisms. We report that CD8+CD122+CD49dlo T regulatory-like cells (CD8+ TRLs) are among the earliest lymphocytes to infiltrate mouse brains after ischemic stroke and temper inflammation; they also confer neuroprotection. TRL depletion worsened outcomes, an effect reversed by CD8+ reconstitution. The CXCR3/CXCL10 axis served as brain-homing mechanism for TRLs....
Background and Purpose: Emerging evidence highlights the importance of IL33 (interleukin 33) its receptor (ST2 [interleukin 1 receptor-like 1]) in normal brains neurological disorders. This study explores function IL33/ST2 signaling axis a transcription factor STAT6 (signal transducer activator 6) white matter integrity long-term recovery after stroke. Methods: Transient middle cerebral artery occlusion was induced wild type, ST2 knockout, microglia/macrophage-depleted (by PLX5622 diet)...
In hippocampal neurons, neurotransmitter release can be regulated by protein kinase A (PKA) through a direct action on the secretory machinery. To identify site of PKA modulation, we have taken advantage ability neurotoxin Botulinum to cleave synaptic SNAP-25. Cleavage this decreases Ca 2+ responsiveness machinery partially uncoupling -sensing from fusion per se . This is expressed as shift toward higher levels relationship and perturbation delay under conditions where secretion induced...
Traumatic brain injury (TBI) is commonly followed by long-term cognitive deficits that severely impact the quality of life in survivors. Recent studies suggest microglial/macrophage (Mi/MΦ) polarization could have multidimensional impacts on post-TBI neurological outcomes. Here, we report repetitive intranasal delivery interleukin-4 (IL-4) nanoparticles for 4 weeks after controlled cortical improved hippocampus-dependent spatial and non-spatial functions adult C57BL6 mice, as assessed a...
Traumatic brain injury (TBI) is commonly followed by intractable psychiatric disorders and long-term changes in affect, such as anxiety. The present study sought to investigate the effect of repetitive intranasal delivery interleukin-4 (IL-4) nanoparticles on affective symptoms after TBI mice. Adult male C57BL/6 J mice (10–12 weeks age) were subjected controlled cortical impact (CCI) assessed a battery neurobehavioral tests up 35 days CCI. Neuron numbers counted multiple limbic structures,...
Lipocalin-2 mediates neuro-inflammation and iron homeostasis in vascular injuries of the central nervous system (CNS) is upregulated extra-CNS systemic inflammation. We postulate that cerebrospinal fluid (CSF) blood lipocalin-2 levels are associated with markers inflammation functional outcome subarachnoid hemorrhage (SAH). prospectively enrolled 67 SAH subjects, serially measured CSF plasma lipocalin-2, matrix metallopeptidase 9 (MMP-9), interleukin-6 (IL-6) tumor necrosis factor-alpha...
In the mammalian cortex, α2 subunit-containing glycine receptors (GlyRs) mediate tonic inhibition, but precise functional role of this type GlyRs is difficult to establish because lack subtype-selective antagonist. study, we found that cyclothiazide (CTZ), an epileptogenic agent, potently inhibited GlyR-mediated current (<i>I</i><sub>Gly</sub>) in cultured rat hippocampal neurons. The inhibition was concentration-dependent, suggesting a competitive mechanism. Note containing not α1 or α3...
Introduction: White matter (WM) injury is an important cause of long-term deficits after stroke. This study explores a novel action IL-4 on promoting WM integrity stroke and elucidates the underlying mechanisms. Hypothesis: promotes repair enhances neurological recovery ischemic by targeting both OPC differentiation microglial polarization. Methods: Cerebral ischemia was induced transient (60 min) middle cerebral artery occlusion (tMCAO) in wild type, knockout oligodendrocyte precursor cell...
May 6, 2019April 9, 2019Free AccessCSF Inflammatory and Vasoactive Mediators Associated with Poor Functional Outcome in Subarachnoid Hemorrhage (SAH) Patients. (S15.004)Aisha Saand, Fang Yu, Jong Lee, Liangge Hsu, MingMing Ning, Eng Lo, Sherry ChouAuthors Info & AffiliationsApril 2019 issue92 (15_supplement)https://doi.org/10.1212/WNL.92.15_supplement.S15.004 Letters to the Editor
May 9, 2019April 2019Free AccessRole of Systemic Inflammatory Response Syndrome (SIRS) in Subarachnoid Hemorrhage (SAH) Outcome (P5.9-040)Yash Pandya, Yazan Bittar, Aisha R Saand, Yu Fang, Murat Kaynar, and Sherry ChouAuthors Info & AffiliationsApril 2019 issue92 (15_supplement)https://doi.org/10.1212/WNL.92.15_supplement.P5.9-040 Letters to the Editor