A5108390869- Platelet Disorders and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Ion Transport and Channel Regulation
- PI3K/AKT/mTOR signaling in cancer
- MicroRNA in disease regulation
- Neonatal and fetal brain pathology
- Mechanisms of cancer metastasis
- Glioma Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Protein Kinase Regulation and GTPase Signaling
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Immunodeficiency and Autoimmune Disorders
- Venous Thromboembolism Diagnosis and Management
- Gut microbiota and health
- NF-κB Signaling Pathways
- Reproductive System and Pregnancy
- Cell Adhesion Molecules Research
- Bone Metabolism and Diseases
- Signaling Pathways in Disease
- Apelin-related biomedical research
- interferon and immune responses
Guang’anmen Hospital
2025
Medical College of Wisconsin
2016-2024
Jiangsu Province Hospital
2024
Jiangnan University
2024
Nanjing University of Chinese Medicine
2024
Versiti Blood Center of Wisconsin
2015-2023
Chinese Academy of Sciences
2020-2022
University of Pittsburgh
2014-2021
Shenzhen Institutes of Advanced Technology
2019-2021
University of Chinese Academy of Sciences
2019-2020
ST2, a member of the interleukin (IL) 1 receptor family, and its ligand IL-33 play critical roles in immune regulation inflammatory responses. This study explores endogenous IL-33/ST2 signaling ischemic brain injury elucidates underlying mechanisms action. The expression rapidly increased oligodendrocytes astrocytes after 60 min transient middle cerebral artery occlusion (tMCAO). ST2 deficiency exacerbated infarction 3 d tMCAO as well distal permanent MCAO. also aggravated neurological...
Delayed thrombolytic treatment with recombinant tissue plasminogen activator (tPA) may exacerbate blood-brain barrier breakdown after ischaemic stroke and lead to lethal haemorrhagic transformation. The immune system is a dynamic modulator of response, excessive cell accumulation in the cerebral vasculature associated compromised integrity barrier. We previously reported that regulatory T cells, which function suppress responses, ameliorated damage ischaemia. This study assessed impact cells...
The repair of white matter damage is paramount importance for functional recovery after brain injuries. Here, we report that interleukin-4 (IL-4) promotes oligodendrocyte regeneration and remyelination. IL-4 receptor expression was detected in a variety glial cells ischemic injury, including lineage cells. deficiency knockout mice resulted greater deterioration over 14 d stroke. Consistent with these findings, intranasal delivery nanoparticles stroke improved integrity attenuated long-term...
Neural stem cell (NSC) transplantation has been proposed to promote functional recovery after spinal cord injury. However, a detailed understanding of the mechanisms how NSCs exert their therapeutic plasticity is lacking. We transplanted mouse into injured seven days SCI, and Basso Mouse Scale (BMS) score was performed assess locomotor function. The anti-inflammatory effects NSC analyzed by immunofluorescence staining neutrophil macrophages detection mRNA levels tumor necrosis factor-α...
Regulatory T cells (Tregs) are known to protect against ischemic stroke. However, the low frequency of Tregs restricts their clinical utility. This study investigated whether expanding number in vivo with IL-2/IL-2 antibody complex (IL-2/IL-2Ab) could improve stroke outcomes and further elaborated mechanisms protection male mice. C57BL/6 mice received IL-2/IL-2Ab or isotype IgG (IsoAb) intraperitoneally for 3 d before (pretreatment) starting 2 h after (posttreatment) 60 min middle cerebral...
Abstract Many autoimmune diseases are characterized by the production of autoantibodies. The current view is that CD4 + T follicular helper (Tfh) cells main subset regulating autoreactive B cells. Here we report a CXCR5 PD1 Tfh CD8 whose development and function negatively modulated Stat5. These regulate germinal center cell response control autoantibody production, as deficiency Stat5 in leads to an increase cells, resulting breakdown tolerance concomitant production. share similar gene...
Sodium–hydrogen exchanger isoform 1 (NHE1) plays a role in survival and migration/invasion of several cancers is an emerging new therapeutic target. However, the NHE1 glioblastoma interaction expression function cells with cytotoxic temozolomide (TMZ) therapy remain unknown. In this study, we detected high levels protein only primary human glioma (GC), xenografts glioblastoma, but not neural stem or astrocytes. GC exhibited alkaline resting pHi (7.46±0.04) maintained by robust NHE1-mediated...
The bumetanide (BMT)-sensitive Na+-K+-2Cl- cotransporter isoform 1 (NKCC1) maintains cell volume homeostasis by increasing intracellular K+ and Cl- content via regulatory increase (RVI). Expression levels of NKCC1 positively correlate with the histological grade severity gliomas, most common primary adult brain tumors, up-regulated activity facilitates glioma migration apoptotic resistance to chemotherapeutic drug temozolomide (TMZ). However, cellular mechanisms underlying functional...
Background Despite recent evidence demonstrating a potent protective effect of adoptively transferred regulatory T cells (Tregs) in ischemic stroke, the mechanism for Treg mobilization and activation brain is, remarkably, unknown. This study determines role C‐C chemokine receptor type 5 ( CCR 5) mediating docking Tregs their protection early blood‐brain barrier disruption after stroke. Methods Results Adoptive transfer −/− failed to reduce infarct or neurological deficits, indicating an...
Microglia play important roles in extracellular matrix remodeling, tumor invasion, angiogenesis, and suppression of adaptive immunity glioma. Na + /H exchanger isoform 1 (NHE1) regulates microglial activation migration. However, little is known about the NHE1 intratumoral microglia–glioma interactions. Our study revealed up-regulation protein expression both glioma cells tumor-associated Iba1 microglia xenografts glioblastoma multiforme microarrays. Moreover, we observed positive correlation...
Long-term neurological recovery after severe traumatic brain injury (TBI) is strongly linked to the repair and functional restoration of injured white matter. Emerging evidence suggests that anti-inflammatory cytokine interleukin-4 (IL-4) plays an important role in promoting matter integrity cerebral ischemic injury. Here, we report delayed intranasal delivery nanoparticle-packed IL-4 boosted sensorimotor a murine model controlled cortical impact, as assessed by battery neurobehavioral tests...
Na+ /H+ exchanger (NHE1) activation is required for multiple microglial functions. We investigated effects of selective deletion Nhe1 in Cx3cr1-CreER ;Nhe1f/f mice on neuroinflammation and tissue repair after ischemic stroke. Infarct volume was similar corn oil or tamoxifen (Tam)-treated at 48 hr 14 days post-stroke. However, the Tam-treated showed significantly higher survival rate faster neurological function recovery during day 1-14 Deletion prevented elevation CD11b+ /CD45low-med...
Abstract The precise molecular mechanism underlying the regulation of early B cell lymphopoiesis is unclear. PLCγ signaling pathway critical for antigen receptor-mediated lymphocyte activation, but its function in cytokine unknown. Here we show that PLCγ1/PLCγ2 double deficiency mice blocks development at pre-pro-B stage and renders progenitors unresponsive to IL-7. inhibition IL-7-induced activation mTOR, not Stat5. activates mTOR through DAG/PKC branch, independent conventional...
Chemerin and G protein-coupled receptor 1 (GPR1) are increased in serum placenta mice during pregnancy. Interestingly, we observed chemerin levels decreased GPR1 expression of high-fat-diet-fed compared with chow-fed at gestational day 18. protein gene were significantly diabetes mellitus (GDM) patient placentas. Therefore, hypothesized that chemerin/GPR1 signaling might participate the pathogenic mechanism GDM. We investigated role carbohydrate homeostasis pregnancy using pregnant...
The WNK-SPAK kinase signaling pathway controls renal NaCl reabsorption and systemic blood pressure by regulating ion transporters channels. A WNK3-SPAK complex is highly expressed in brain, but its function this organ remains unclear. Here, we investigated the role of brain edema white matter injury after ischemic stroke. Wild-type, WNK3 knockout, SPAK heterozygous or knockout mice underwent transient middle cerebral artery occlusion. One cohort magnetic resonance imaging. Ex-vivo brains...
Immune thrombocytopenia (ITP) is an autoimmune disease which arises due to self-destruction of circulating platelets. Failure respond or maintain a response first-line treatment can lead refractory/relapsed (R/R) ITP. The mechanism remains complicated and lacks standard clinical treatment. Sirolimus (SRL) mammalian target rapamycin (mTOR) inhibitor that has been demonstrated inhibit lymphocyte activity, indicating potential for SRL in Activation the mTOR pathway diseases suggests might be...
Myasthenia gravis (MG), a neuromuscular junction autoimmune disorder, causes skeletal muscle weakness. MG worsening frequently occurs during the disease course, severely impairing quality of life and elevating myasthenic crisis risk. Existing predictive models remain scarce. This study developed model for to facilitate early risk stratification personalized care. Retrospective analysis included 437 Gravis Foundation America (MGFA) class I - III myasthenia patients from December 2019...
Abstract The three major Ras members, Kras, Hras, and Nras, are highly homologous individual genes can have distinct biological functions. Embryonic lethality of Kras-deficient mice precludes study the functions this family member. In study, we generated examined with hematopoietic-specific deletion Kras bone marrow (BM) chimeric B cell–specific targeted Kras. Hematopoietic-specific impaired early cell development at pre–B stage late maturation, resulting in reduction BM pre–, immature,...
Abstract Systemic lupus erythematosus ( SLE ) is an autoimmune disease, which results in various organ pathologies. However, current treatment towards suboptimal. Erythropoietin EPO has been shown to promote recovery, but clinical application can be limited by its haematopoiesis‐stimulating effects. ‐derived helix‐B peptide ARA 290) non‐erythrogenic reported retain the anti‐inflammatory and tissue‐protective functions of . Therefore, here we investigated effects potential mechanisms 290 on...
The adipokine chemerin has been considered an important regulator of tumor immune surveillance. Chemerin recruits leukocytes through the receptor CMKLR1 to improve clinical outcomes tumors and overall patient survival, but role GPR1 in not widely investigated. Here, we found that expression is elevated breast cancer-especially triple-negative cancer (TNBC) tissues cell lines. Herein, screened a phage display peptide library identify LRH7-G5, antagonist blocks chemerin/GPR1 signaling. This...
Heparin-induced thrombocytopenia is a relatively common drug-induced immune disorder that can have life-threatening consequences for affected patients. Immune complexes consisting of heparin, platelet factor 4 (PF4), and PF4/heparin-reactive Abs are central to the pathogenesis heparin-induced thrombocytopenia. Regulatory T (Treg) cells subpopulation CD4 play key role in regulating responses, but their controlling PF4/heparin-specific Ab production unknown. In studies described this article,...
BACKGROUNDCytokine biomarkers have already been used to predict acute graft-versus-host disease (aGVHD) onset, nonrelapse mortality, and overall survival in human mouse models, but the consistency of consequences between patients mice has not evaluated. Furthermore, no study about any biomarker or panel for aGVHD grading steroid sensitivity simultaneously reported.METHODSHere we established an model explored relation onset variations some cytokines. Based on results latest progress, selected...