A5064937029- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Cancer Research and Treatments
- Clostridium difficile and Clostridium perfringens research
- Infant Nutrition and Health
- Mycobacterium research and diagnosis
- Plant Pathogenic Bacteria Studies
- Infant Health and Development
- Neuroscience and Neuropharmacology Research
- Drug Transport and Resistance Mechanisms
- Bacterial Identification and Susceptibility Testing
- Amino Acid Enzymes and Metabolism
- Aerosol Filtration and Electrostatic Precipitation
- Metabolism and Genetic Disorders
- Dietary Effects on Health
- Environmental and Sediment Control
- Nanoplatforms for cancer theranostics
- Cancer, Stress, Anesthesia, and Immune Response
- Gastrointestinal motility and disorders
RIKEN Center for Brain Science
2022-2024
Tokyo University of Agriculture and Technology
2018-2022
RIKEN
2018
RIKEN Nishina Center
2018
Six strains of Gram-stain-negative, obligately anaerobic, non-spore-forming, non-motile rods were isolated from human faeces. Based on phylogenetic characteristics, the six isolates included in family Ruminococcaceae, and divided into three groups. The showed 16S rRNA gene sequence similarity values lower than 96.2 % to closely related species, Oscillibacter ruminantium GH1T, valericigenes Sjm18-20T Dysosmobacter welbiomis J115T. Coherently with results, silico DNA-DNA hybridization average...
Abstract Amino acids participate directly and indirectly in many important biochemical functions the brain. We focused on one amino acid metabolic enzyme, L-amino oxidase (LAO), investigated importance of LAO brain function using LAO1 knockout (KO) mice. Compared to wild-type mice, KO mice exhibited impaired fear learning memory a passive avoidance test. This impairment coincided with significantly reduced hippocampal acetylcholine levels compared while treatment donepezil, reversible...
Chronic diarrhea in laboratory-bred marmosets poses a serious health problem during experiments. Despite growing demand for experimental marmosets, the mechanisms underlying development of and measures its treatment prevention remain unclear. To explore factors affecting chronic gut microbiota composition (GMC) 58 including 19 animals with diarrhea, was analyzed using terminal restriction fragment length polymorphism. We found that GMCs these cluster into two groups differ significantly rate...
Two strictly anaerobic strains (MB11T and MB56) were isolated from common marmoset (Callithrixjacchus) faeces. Cells of the two Gram-stain-negative, pleomorphic short (strain MB11T) or long rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates related to genus Phascolarctobacterium. They had similarities lower than 93 % previously described species, Phascolarctobacterium faecium ACM 3679T succinatutens YIT 12067T, 98.7 between themselves. DNA-DNA...
The development of gut microbiota during infancy is an important event that affects the health status host; however, mechanism governing it not fully understood, l-Amino acid oxidase 1 (LAO1) a flavoprotein catalyzes oxidative deamination particular l-amino acids and converts them into keto acids, ammonia, H2O2. Our previous study showed LAO1 present in mouse milk exerts protection against bacteria by its production data led us to consider whether LAO1, H2O2, or both could impact infant via...
Wasting marmoset syndrome (WMS), a serious disease in captive common (Callithrix jacchus) colonies, is associated with high mortality rate. The specific cause of WMS still unclear and there are few effective treatments. Previously, we had reported tranexamic acid therapy supportive measures as useful treatment for WMS. In the present study, describe modified method: combination 0.1 mL 5% subcutaneously five times per week, 2.0 amino formulation intravenously three 5.0 Ringer's lactate...
We demonstrate that Blautia coccoides JCM1395T has the potential to be used for tumor-targeted live bacterial therapeutics. Prior studying its in vivo biodistribution, a sample preparation method reliable quantitative analysis of bacteria biological tissues was required. Gram-positive have thick outer layer peptidoglycans, which hindered extraction 16S rRNA genes colony PCR. developed following solve issue; we is as follows. The homogenates isolated tissue were seeded on agar medium, and...
Abstract Gut microbiota is associated with antitumor efficacy of immune checkpoint blockade therapies, yet the mechanism not largely understood. Here, we show that a novel bacterial strain Ruminococcaceae isolated from feces patients who respond to PD-1 (programmed cell death 1) efficiently primes/activates tumor-specific CD8+ T cells through effective stimulation/maturation CD103+ dendritic (DCs) during migration gut tumor microenvironment (TME). The administration this bacteria...
Laboratory animals are used for scientific research in various fields. In recent years, there has been a concern that the gut microbiota may differ among laboratory animals, which yield different results laboratories where in-vivo experiments performed. Our knowledge of laboratory-reared common marmosets (Callithrix jacchus) is limited; thus, this study, we analyzed daily changes fecal microbiome composition, individual variations, and effects birth facility healthy female marmosets,...