A5103102260- Influenza Virus Research Studies
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Animal Disease Management and Epidemiology
- Mitochondrial Function and Pathology
- Respiratory viral infections research
- Viral Infections and Vectors
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Genetic and Kidney Cyst Diseases
- Viral gastroenteritis research and epidemiology
- DNA Repair Mechanisms
- Tissue Engineering and Regenerative Medicine
- Animal Virus Infections Studies
- Protist diversity and phylogeny
- Cancer therapeutics and mechanisms
- Analytical Chemistry and Chromatography
- Photosynthetic Processes and Mechanisms
- Reproductive System and Pregnancy
- Renal and related cancers
- Plant Molecular Biology Research
- Infectious Encephalopathies and Encephalitis
- Virus-based gene therapy research
- Mesenchymal stem cell research
- Radiomics and Machine Learning in Medical Imaging
Monash University
2022-2024
Gyeongsang National University
2021
Chungbuk National University
2015-2019
Medical Research Institute
2015
Background. Although Middle East Respiratory Syndrome coronavirus (MERS-CoV) is characterized by a risk of nosocomial transmission, the detailed mode transmission and period virus shedding from infected patients are poorly understood. The aims this study were to investigate potential role environmental contamination MERS-CoV in healthcare settings define viable MERS patients.
Abstract The development of oocytes and early embryos is dependent on mitochondrial ATP production. This reliance activity, together with the exclusively maternal inheritance mitochondria in development, places as central regulators both fertility transgenerational mechanisms. Mitochondrial mass mtDNA content massively increase during oocyte growth. They are highly dynamic organelles maturation accompanied by trafficking around subcellular compartments. Due to their key roles generation...
Eggs contain about 200,000 mitochondria that generate adenosine triphosphate and metabolites essential for oocyte development. Mitochondria also integrate metabolism transcription via regulate epigenetic modifiers, but there is no direct evidence linking mitochondrial function to the maternal epigenome subsequent embryo Here, we have disrupted deletion of fission factor Drp1. Fission-deficient oocytes exhibit a high frequency failure in peri- postimplantation This associated with altered...
Highly pathogenic avian influenza (HPAI) A(H5N6) and A(H5N8) virus infections resulted in the culling of more than 37 million poultry Republic Korea during 2016/17 winter season. Here we characterize two representative viruses, A/Environment/Korea/W541/2016 [Em/W541(H5N6)] A/Common Teal/Korea/W555/2017 [CT/W555(H5N8)], evaluate their zoonotic potential various animal models. Both Em/W541(H5N6) CT /W555(H5N8) are novel reassortants derived from gene pools wild bird viruses present migratory...
A dispersed cytoplasmic distribution of mitochondria is a hallmark normal cellular organization. Here, we have utilized the expression exogenous Trak2 in mouse oocytes and embryos to disrupt by driving them into large aggregate. Our findings reveal that aggregated minimal impact on asymmetric meiotic cell divisions oocyte. In contrast, during first mitotic division result daughter cells with unequal sizes increased micronuclei. Further, two-cell embryos, microtubule-mediated centering...
Oocyte maturation and embryonic development are sensitive to DNA damage. Compared with somatic cells or oocytes, little is known about the response damage in early preimplantation embryos. In this study, we examined checkpoints repair mechanisms parthenogenetic porcine We found that most of etoposide-treated embryos showed delay cleavage ceased before blastocyst stage. DNA-damaged embryos, earliest positive TUNEL signals were detected on Day 5 vitro culture. Caffeine, which an ATM (ataxia...
Mammalian oocytes lack a centriole that acts as microtubule organization center (MTOC) in most somatic cells. During oocyte maturation, MTOCs undergo remodeling processes, including decondensation, fragmentation, and self-organization. However, the underlying mechanisms of MTOC mouse are not well understood. We showed two pericentriolar proteins, Cep192 Cep152, play crucial roles during oocytes. is present at all stages its depletion induces ablation MTOCs, delay spindle formation, abnormal...
In order to produce a dually effective vaccine against H9 and H5 avian influenza viruses that aligns with the DIVA (differentiating infected from vaccinated animals) strategy, we generated chimeric H9/H5N2 recombinant expressed whole HA1 region of A/CK/Korea/04163/04 (H9N2) HA2 recent highly pathogenic (HPAI) A/MD/Korea/W452/14 (H5N8) viruses. The virus showed in vitro vivo growth properties virulence were similar those low-pathogenic (LPAI) virus. An inactivated based on this induced serum...
In somatic cells spindle microtubules are nucleated from centrosomes that act as major microtubule organizing centers (MTOCs), whereas oocytes form meiotic spindles by assembling multiple acentriolar MTOCs without canonical centrosomes. Aurora A and Plk1 required for these events, but the underlying mechanisms remain largely unknown. Here we show CIP2A regulates MTOC organization recruiting aurora at poles during maturation. colocalized with pericentrin a few distinct cytoplasmic foci....
Recent canine influenza outbreaks have raised concerns about the generation of pathogenic variants that may pose a threat to public health. Here, we examine avian-like H3N2 viruses (CIVs) isolated from 2009 2013 in South Korea dogs. Phylogenetic analysis revealed these are closely related strains previously dogs and China. However, molecular characterization demonstrated non-synonymous mutations between viruses, particularly putative H3 antigenic sites, NA stalk regions, internal genes...
Abstract The SUN (Sad-1/UNC-84) and KASH (Klarsicht/ANC-1/Syne/homology) proteins constitute the linker of nucleoskeleton cytoskeleton (LINC) complex on nuclear envelope. To date, SUN1/KASH5 is known to function as meiotic-specific factors. In this study, gene-silencing methods were used explore roles SUN1 KASH5 in mouse oocytes after prophase. was detected throughout nucleus; however, dispersed through cell. After germinal vesicle breakdown (GVBD), migrated during spindle formation...
Mitochondria are dynamic organelles that undergo regulated microtubule- and actin-mediated trafficking to meet local energy metabolic needs. Mitochondrial may be particularly critical in large cells such as eggs early embryos where spindle formation polar body extrusion occur specific regions of the cytoplasm. To investigate role mitochondrial distribution oocytes we have targeted membrane protein, MIRO1, which couples mitochondria motor protein-TRAK complex. Oocyte-specific deletion MIRO1...
Recently identified highly pathogenic avian influenza (HPAI) H5N8 viruses (clade 2.3.4.4) are relatively low to moderately in mammalian hosts compared with HPAI H5N1 viruses. In this study, we generated reassortant comprised of A/MD/Korea/W452/2014(H5N8) substitution individual genes from A/EM/Korea/W149/2006(H5N1) understand the contribution each viral gene virulence mammals. Substituting PB2 segment or NA virus by that resulted significantly enhanced pathogenicity parental mice. Of note, a...
Zinc plays an essential role in mammalian oocyte maturation, fertilization, and early embryogenesis, depletion of zinc impairs cell cycle control, asymmetric division, cytokinesis oocyte. We report that zinc, via the actin nucleator Spire, acts as regulator cytoskeleton remodeling during mouse maturation fertilization. Depletion impaired cortical cytoplasmic formation. Spire is colocalized with zinc-containing vesicles its finger-containing Fab1, YOTB, Vac 1, EEA1 (FYVE) domain. Improper...
Unlike somatic cells mitosis, germ cell meiosis consists of 2 consecutive rounds division that segregate homologous chromosomes and sister chromatids, respectively. The meiotic oocyte is characterized by an absence centrioles asymmetric division. Centriolin a relatively novel centriolar protein functions in mitotic cycle progression cytokinesis. Here, we explored the function centriolin showed it localized to spindles concentrated at spindle poles midbody during maturation. Unexpectedly,...
Due to increasing concerns about human infection by various H7 influenza viruses, including recent H7N9 we evaluated the genetic relationships and cross-protective efficacies of three different Eurasian avian viruses. Phylogenic molecular analyses revealed that viruses can be separated into two lineages, with relatively high amino acid identities within groups (94.8 98.8%) low between (90.3 92.6%). In vivo immunization representatives each group while group-specific cross-reactivity was...
Abstract Mammalian oocytes lack centrioles but can generate bipolar spindles using several different mechanisms. For example, mouse have acentriolar microtubule organization centers (MTOCs) that contain many components of the centrosome, and which initiate polymerization. On contrary, human MTOCs Ran‐mediated mechanisms may be responsible for spindle assembly. Complete knowledge assembly is lacking in various mammalian oocytes. In this study, we demonstrate both MTOC‐ nucleation are required...
Pluripotent stem cells (PSCs) have the ability of self-renewal that can retain characteristics mother cell, and pluripotency differentiate into several body types. PSCs typically include embryonic (ESCs) derived from inner cell mass preimplantation embryo, epiblast (EpiSCs) postimplantation embryo. Although are able to be used by differentiation endothelial as a potential treatment for vascular diseases, human ESCs induced (iPSCs) followed ethical safety issues. Pigs anatomically...