A5042914571- Antibiotic Resistance in Bacteria
- Pneumocystis jirovecii pneumonia detection and treatment
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Mycobacterium research and diagnosis
- Pediatric health and respiratory diseases
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Chronic Obstructive Pulmonary Disease (COPD) Research
- HIV/AIDS Research and Interventions
- Amino Acid Enzymes and Metabolism
- Streptococcal Infections and Treatments
- Bacteriophages and microbial interactions
- Nitric Oxide and Endothelin Effects
- Neutropenia and Cancer Infections
- Enterobacteriaceae and Cronobacter Research
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Biosensors and Analytical Detection
- Clostridium difficile and Clostridium perfringens research
- COVID-19 Clinical Research Studies
Maastricht University
2014-2024
Maastricht University Medical Centre
2020-2023
University Medical Center
2015
GGD Zuid Limburg
2014
We investigated the effect of international travel on gut resistome 122 healthy travelers from Netherlands by using a targeted metagenomic approach. Our results confirm high acquisition rates extended-spectrum β-lactamase encoding gene blaCTX-M, documenting rise in prevalence 9.0% before to 33.6% after (p<0.001). The quinolone resistance genes qnrB and qnrS increased 6.6% 8.2% 36.9% 55.7% travel, respectively (both p<0.001). Travel Southeast Asia Indian subcontinent was associated with...
Recently, the first plasmid-mediated colistin-resistance gene, mcr-1, was reported. Colistin is increasingly used as an antibiotic of last resort for treatment infections caused by carbapenem-resistant bacteria, which have been rapidly disseminating worldwide in recent years.The reported carriage rate mcr-1 humans remains sporadic thus far, except those Chinese populations. We aimed to determine its presence faecal metagenomes healthy Dutch travellers between 2010 and 2012.Faecal metagenomic...
Abstract There has been a growing body of evidence that the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant (B.1.617.2) shows enhanced transmissibility and increased viral loads compared to other variants. A recent study even suggested samples from people infected with can harbor up 1000 times higher variants are more closely related original Wuhan strain, although sample size this (n = 125) was very limited. Here, we have load in 16,185 were obtained periods...
Abstract We investigated the effect of international travel on gut resistome 122 healthy travelers from Netherlands by using a targeted metagenomic approach. Our results confirm high acquisition rates extended-spectrum β-lactamase encoding gene blaCTX-M, documenting rise in prevalence 9.0% before to 33.6% after (p<0.001). The quinolone resistance genes qnrB and qnrS increased 6.6% 8.2% 36.9% 55.7% travel, respectively (both p<0.001). Travel Southeast Asia Indian subcontinent was associated...
Enhanced arginase-induced arginine consumption is believed to play a key role in the pathogenesis of sickle cell disease-induced end organ failure. Enhancement availability with L-arginine supplementation exhibited less consistent results; however, L-citrulline, precursor L-arginine, may be promising alternative. In this study, we determined effects L-citrulline compared on arginine-nitric oxide (NO) metabolism, and microcirculation murine model acutely-enhanced arginase activity. The were...
Abstract A two-step strategy combining assisted benchmark testing (entry controls) and External Quality Assessments (EQAs) with blinded simulated clinical specimens to enhance maintain the quality of nucleic acid amplification was developed. This successfully applied 71 diagnostic laboratories in The Netherlands when upscaling national capacity during SARS-CoV-2 pandemic. availability combination advice for improvement substantially enhanced laboratory procedures detection. three subsequent...
Abstract There has been a growing body of evidence that the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant (B.1.617.2) shows enhanced transmissibility and increased viral loads compared to other variants. A recent study even suggested samples from people infected with can harbor up 1000 times higher variants are more closely related original Wuhan strain, although sample size this was very limited. Here, we have load in 16,185 were obtained during which non-VOC,...
Aim: We investigated the longitudinal development of several antibiotic resistance genes (ARGs) infant gut resistome during first months after birth. Materials & methods: Fecal samples from 120 infants collected at ages 5, 13 and 31 weeks were analyzed subjected to qPCR for detection ARGs. Results: The prevalence ARGs significantly increased ermB, tetM tetQ, while it decreased aac(6′)-aph(2′). Birth mode breastfeeding affected tetQ prevalence. Correlations bacterial taxa suggest that...
In 2019-2020, two subsequent outbreaks caused by phenotypically identical ESBL-producing Enterobacter cloacae and multi-drug-resistant (MDR) Pseudomonas putida were detected in respectively 15 9 patients of the haematology-oncology department. Both bacterial species resistant to piperacillin-tazobactam, used empirically (neutropenic) sepsis our hospital, ciprofloxacin, prophylactically selective digestive decontamination for haematology patients. The E. outbreak was identified clinical...
Abstract Vancomycin-resistant enterococci (VRE) can rapidly spread through hospitals. Therefore, our hospital employs a screening program whereby rectal swabs are screened for the presence of vanA and vanB , only PCR-positive broths cultured on VRE selection agar. Early November 2016, clinical vanA- / -negative isolate was detected in vanA/vanB -screening-negative patient, giving possibility that an undetected might be spreading within hospital. Whole-genome-sequencing showed resistance vanD...
Genotyping of Klebsiella pneumoniae is indispensable for management nosocomial infections, monitoring emerging strains--including extended-spectrum beta-lactamase (ESBL) producers-, and general epidemiology. Such objectives require a high-resolution genotyping method with fixed scheme that allows (1) long-term retrospective prospective assessment, (2) objective result readout (3) library storage database development exchangeable results. We have developed multiple-locus variable number...
The human gut microbiome is an important reservoir of antimicrobial resistance genes (ARGs), collectively termed the 'resistome'. To date, few studies have examined dynamics resistome in healthy individuals. Previously, authors observed high rates ARG acquisition and significant abundance shifts during international travel. In order to provide insight into commonly occurring dynamics, this study investigated longitudinal fluctuations prevalent ARGs (cfxA, tetM ermB) resistomes non-travelling...
Maintenance treatment with macrolide antibiotics has shown to be effective in reducing exacerbations COPD patients. A major concern prolonged is the development of bacterial resistance. In this study we determined effect azithromycin on and acquisition resistance macrolides nasopharyngeal flora
We evaluated routine testing with SARS-CoV-2 Delta variant-specific RT-PCR in regional hospital laboratories addition to centralised national genomic surveillance the Netherlands during June and July 2021. The increase of variant detected by correlated well data from was available ca 2 weeks earlier. This rapid identification relative abundance variants concern may have important benefits for implementation local public health measures.
Abstract During the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, several variants of concern (VOC) have emerged and became dominant strain. These are considered VOC because traits like increased transmissibility, severity, immune-evasion. Understanding their viral load dynamics, with use longitudinal follow-up data, can help to understand transmission could inform policy makers on for example infection prevention guidelines. However, studies scarce. In this study,...
Abstract ObjectivesMaintenance treatment with macrolide antibiotics has shown to be effective in reducing exacerbations COPD patients. A major concern prolonged is the development of bacterial resistance. In this study we determined effect azithromycin on and acquisition resistance macrolides nasopharyngeal flora patients.MethodsThis was part COLUMBUS trial, a randomised, double-blind, placebo-controlled trial measure maintenance 92 patients exacerbation rates during 12-month period. order...
Background: The patient environment is increasingly considered a major source of transmission nosocomial bacteria to patients. In May 2019, cluster 3 patients with multiresistant Enterobacter cloacae was discovered in the hematology-oncology department Maastricht University Medical Center (built 1991). strains had an identical antibiogram: ESBL-positive, ciprofloxacin R, cotrimoxazole meropenem S, and colistin S. One neutropenic positive blood culture for this strain, resistant empiric...
<b>Objectives:</b> A major concern with prolonged treatment antibiotics is the development of bacterial resistance. In this study we determined effect azithromycin on and acquisition resistance to macrolides in nasopharyngeal flora COPD patients. <b>Methods:</b> This was part COLUMBUS trial, a randomised, double-blind, placebo-controlled trial measure maintenance 92 patients exacerbation rates during 12-month period. order determine macrolides, used targeted metagenomic approach presence...
Abstract Objectives : Maintenance treatment with macrolide antibiotics has shown to be effective in reducing exacerbations COPD patients. A major concern prolonged is the development of bacterial resistance. In this study we determined effect azithromycin on and acquisition resistance macrolides nasopharyngeal flora Methods This was part COLUMBUS trial, a randomised, double-blind, placebo-controlled trial measure maintenance 92 patients exacerbation rates during 12-month period. order...